Strain Name:
C57BL/6J-MtgxR5279Btlr/Mmmh
Stock Number:
042839-MU
Citation ID:
RRID:MMRRC_042839-MU
Other Names:
R5279 (G1), C57BL/6J-MtgxR5279Btlr
Major Collection:

Strain Information

Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Gad2
Name: glutamic acid decarboxylase 2
Synonyms: GAD65, Gad-2, 6330404F12Rik, GAD(65)
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14417
HGNC: HGNC:4093
Homologene: 20223
Rpa1
Name: replication protein A1
Synonyms: RF-A, 5031405K23Rik, 70kDa, RP-A, Rpa
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68275
Homologene: 2208
Gon4l
Name: gon-4 like
Synonyms: 2610100B20Rik, 1500041I23Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76022
Homologene: 13002
Pdia3
Name: protein disulfide isomerase associated 3
Synonyms: Grp58, ERp60, ERp57, PDI-Q2, PDI, Erp, Plca, ERp61
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14827
HGNC: HGNC:4606
Homologene: 68454
Cdc6
Name: cell division cycle 6
Synonyms: CDC18L, CDC18(S.pombe), cell division cycle 18 homolog (S.pombe)-like
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 23834
HGNC: HGNC:1744
Homologene: 68172
Rbm6
Name: RNA binding motif protein 6
Synonyms: g16, NY-LU-12, Def-3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 19654
HGNC: HGNC:9903
Homologene: 31336
Pard3
Name: par-3 family cell polarity regulator
Synonyms: ASIP, PAR-3, D8Ertd580e, Pard3a, Par3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 93742
Homologene: 10489
Triobp
Name: TRIO and F-actin binding protein
Synonyms: Tara, Mus EST 478828, EST478828
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110253
Homologene: 5104
Birc6
Name: baculoviral IAP repeat-containing 6
Synonyms: D630005A10Rik, apollon, A430032G04Rik, A430040A19Rik, Bruce
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12211
Homologene: 7248
Zc3h3
Name: zinc finger CCCH type containing 3
Synonyms: Smicl
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223642
VEGA: 15
Homologene: 9029
Xpo4
Name: exportin 4
Synonyms: B430309A01Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 57258
Homologene: 10733
Atf7ip
Name: activating transcription factor 7 interacting protein
Synonyms: Mcaf1, ATFa-associated Modulator, AM, 2610204M12Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 54343
Homologene: 10051
Snx30
Name: sorting nexin family member 30
Synonyms: 4732481H14Rik, C030041J06Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 209131
Homologene: 2811
Afdn
Name: afadin, adherens junction formation factor
Synonyms: AF6, S-afadin, 5033403D15Rik, Afadin, I-afadin, Mllt4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17356
HGNC: HGNC:7137
Homologene: 21202
Fip1l1
Name: factor interacting with PAPOLA and CPSF1
Synonyms: Rje, 1300019H17Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 66899
Homologene: 136254
Atp9b
Name: ATPase, class II, type 9B
Synonyms: IIb
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 50771
VEGA: 18
Homologene: 21915
Ankrd28
Name: ankyrin repeat domain 28
Synonyms: E430019N21Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105522
VEGA: 14
Homologene: 35374
Pikfyve
Name: phosphoinositide kinase, FYVE type zinc finger containing
Synonyms: Pip5k3, PipkIII, 5230400C17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18711
Homologene: 32115
Mcm3ap
Name: minichromosome maintenance complex component 3 associated protein
Synonyms: GANP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 54387
VEGA: 10
HGNC: HGNC:6946
Homologene: 2902
Tceanc2
Name: transcription elongation factor A (SII) N-terminal and central domain containing 2
Synonyms: Tdeanc2, 2210012G02Rik, 6330404A07Rik, 2210010B22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66526
Homologene: 11984
Ntn1
Name: netrin 1
Synonyms: Netrin-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18208
HGNC: HGNC:8029
Homologene: 21008
Abca17
Name: ATP-binding cassette, sub-family A member 17
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 381072
Homologene: 86807
Calcoco1
Name: calcium binding and coiled coil domain 1
Synonyms: Gcap11, 1810009B06Rik, CoCoA
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 67488
Homologene: 10845
Plod2
Name: procollagen lysine, 2-oxoglutarate 5-dioxygenase 2
Synonyms: LH2, D530025C14Rik, Plod-2, lysyl hydroxylase 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 26432
HGNC: HGNC:9082
Homologene: 22681
Cdc20
Name: cell division cycle 20
Synonyms: p55CDC, 2310042N09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 107995
HGNC: HGNC:1723
Homologene: 37459
AW554918
Name: expressed sequence AW554918
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225289
Homologene: 52652
Kat6a
Name: K(lysine) acetyltransferase 6A
Synonyms: Myst3, 9930021N24Rik, Zfp220, MOZ
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244349
Homologene: 4924
Baz2b
Name: bromodomain adjacent to zinc finger domain, 2B
Synonyms: 5830435C13Rik, D2Ertd794e
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 407823
HGNC: HGNC:963
Homologene: 8394
Tyw3
Name: tRNA-yW synthesizing protein 3 homolog (S. cerevisiae)
Synonyms: 5230400J09Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 209584
Homologene: 44531
Sugp2
Name: SURP and G patch domain containing 2
Synonyms: Sfrs14, Srsf14
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234373
Homologene: 8923
Robo4
Name: roundabout guidance receptor 4
Synonyms: 1200012D01Rik, Magic roundabout
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74144
Homologene: 10397
Susd4
Name: sushi domain containing 4
Synonyms: E430021N18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 96935
Homologene: 23062
Primpol
Name: primase and polymerase (DNA-directed)
Synonyms: Ccdc111
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 408022
Homologene: 14065
Ttn
Name: titin
Synonyms: shru, L56, mdm, connectin, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, D830007G01Rik, 1100001C23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Cdh13
Name: cadherin 13
Synonyms: T-cadherin, 4932416G01Rik, Tcad
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12554
HGNC: HGNC:1753
Homologene: 20335
Itga9
Name: integrin alpha 9
Synonyms: 6720458D17Rik, 2610002H11Rik, D9Ertd428e
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 104099
HGNC: HGNC:6145
Homologene: 1664
Pcsk5
Name: proprotein convertase subtilisin/kexin type 5
Synonyms: b2b585Clo, PC6, SPC6, PC5/6A, PC5A, b2b1549Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18552
VEGA: 19
HGNC: HGNC:8747
Homologene: 21244
Sdk2
Name: sidekick cell adhesion molecule 2
Synonyms: 4632412F08Rik, 5330435L01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237979
Homologene: 10406
Trappc11
Name: trafficking protein particle complex 11
Synonyms: D030016E14Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 320714
Homologene: 11076
Mrc1
Name: mannose receptor, C type 1
Synonyms: MR, CD206
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17533
HGNC: HGNC:7228
Homologene: 37622
Carmil3
Name: capping protein regulator and myosin 1 linker 3
Synonyms: Lrrc16b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 268747
VEGA: 14
Homologene: 74564
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239420
Homologene: 65982
C8a
Name: complement component 8, alpha polypeptide
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230558
HGNC: HGNC:1352
Homologene: 472
Ttll9
Name: tubulin tyrosine ligase-like family, member 9
Synonyms: 4930509O20Rik, 1700016F23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74711
Homologene: 41769
Ceacam23
Name: CEA cell adhesion moleculen23
Synonyms: Gm5155
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381852
HGNC: HGNC:1819
Homologene: 115938
Zxdc
Name: ZXD family zinc finger C
Synonyms: B930086F11Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 80292
Homologene: 82340
Stard7
Name: StAR related lipid transfer domain containing 7
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99138
Homologene: 32463
Pklr
Name: pyruvate kinase liver and red blood cell
Synonyms: R-PK, Pk1, Pk-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18770
HGNC: HGNC:9020
Homologene: 37286
Vmn2r104
Name: vomeronasal 2, receptor 104
Synonyms: V2r7
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22313
Homologene: 129751
Csmd2
Name: CUB and Sushi multiple domains 2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 329942
Homologene: 89034
Lrrc9
Name: leucine rich repeat containing 9
Synonyms: 4930432K16Rik, 4921529O18Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 78257
Homologene: 12692
Smarca2
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Synonyms: 2610209L14Rik, Snf2l2, brm
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 67155
Homologene: 2308
Nkiras1
Name: NFKB inhibitor interacting Ras-like protein 1
Synonyms: 2400004O09Rik, KBRAS1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 69721
Homologene: 10670
Fpgs
Name: folylpolyglutamyl synthetase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14287
HGNC: HGNC:3824
Homologene: 6997
Nr2c2ap
Name: nuclear receptor 2C2-associated protein
Synonyms: 2310073E15Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 75692
Homologene: 18532
Ptpru
Name: protein tyrosine phosphatase receptor type U
Synonyms: RPTPlambda, Ptprl
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19273
HGNC: HGNC:9683
Homologene: 4168
Mst1
Name: macrophage stimulating 1 (hepatocyte growth factor-like)
Synonyms: D3F15S2h, D9H3F15S2, Hgfl, DNF15S2h
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 15235
Homologene: 7360
Vmn2r65
Name: vomeronasal 2, receptor 65
Synonyms: ENSMUSG00000070600
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100009609
Rgl2
Name: ral guanine nucleotide dissociation stimulator-like 2
Synonyms: Rlf, KE1.5, Rgt2, Rab2l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 19732
HGNC: HGNC:9769
Homologene: 3494
Fam136a
Name: family with sequence similarity 136, member A
Synonyms: 2010309E21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66488
Homologene: 135942
Krtap4-25
Name: keratin associated protein 4-25
Synonyms: 2310045F10Rik, Gm11563
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 100040248
Homologene: 136093
Rnf8
Name: ring finger protein 8
Synonyms: AIP37, 3830404E21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 58230
Homologene: 2944
Rps4l
Name: ribosomal protein S4-like
Synonyms: 1110033J19Rik, Rps4y2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 66184
Tm4sf4
Name: transmembrane 4 superfamily member 4
Synonyms: il-TMP
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229302
Homologene: 3393
Spx
Name: spexin hormone
Synonyms: B230216G23Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 319552
Homologene: 79576
Psmc3
Name: proteasome (prosome, macropain) 26S subunit, ATPase 3
Synonyms: Tat binding protein 1, TBP-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19182
HGNC: HGNC:9549
Homologene: 2097
Vmn1r65
Name: vomeronasal 1 receptor 65
Synonyms: V3R6, V1rd6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 81013
Homologene: 110799
Dnah7c
Name: dynein, axonemal, heavy chain 7C
Synonyms: Dnahc7c
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 100101919
Homologene: 41287
Fzd4
Name: frizzled class receptor 4
Synonyms: Fz4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14366
HGNC: HGNC:4042
Homologene: 7325
Pcdhga5
Name: protocadherin gamma subfamily A, 5
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93713
HGNC: HGNC:8703
Homologene: 69263
Kdm6bos
Name: KDM1 lysine (K)-specific demethylase 6B, opposite strand
Synonyms: 1700064E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 46,519,269 bp
  • A to T, chromosome 1 at 65,196,699 bp
  • C to T, chromosome 1 at 182,887,478 bp
  • T to C, chromosome 2 at 14,310,058 bp
  • A to G, chromosome 2 at 22,673,957 bp
  • A to C, chromosome 2 at 32,692,767 bp
  • G to T, chromosome 2 at 59,932,152 bp
  • T to C, chromosome 2 at 76,900,976 bp
  • T to A, chromosome 2 at 91,054,322 bp
  • T to C, chromosome 2 at 121,414,003 bp
  • T to C, chromosome 2 at 127,295,496 bp
  • T to A, chromosome 2 at 152,962,544 bp
  • T to G, chromosome 3 at 57,433,738 bp
  • A to G, chromosome 3 at 88,887,637 bp
  • A to G, chromosome 3 at 89,143,259 bp
  • A to G, chromosome 3 at 154,594,471 bp
  • A to G, chromosome 4 at 59,885,070 bp
  • T to G, chromosome 4 at 104,845,988 bp
  • T to A, chromosome 4 at 107,177,629 bp
  • A to G, chromosome 4 at 118,433,514 bp
  • T to A, chromosome 4 at 128,456,914 bp
  • T to A, chromosome 4 at 131,820,023 bp
  • A to C, chromosome 5 at 74,585,098 bp
  • T to A, chromosome 6 at 86,366,704 bp
  • T to C, chromosome 6 at 90,370,437 bp
  • T to A, chromosome 6 at 136,603,379 bp
  • A to G, chromosome 6 at 142,414,040 bp
  • C to T, chromosome 6 at 148,355,131 bp
  • A to G, chromosome 7 at 6,008,755 bp
  • T to A, chromosome 7 at 17,873,289 bp
  • A to C, chromosome 7 at 84,940,641 bp
  • G to A, chromosome 7 at 89,407,673 bp
  • CGCAGCAGCAGCAGCAGCA to CGCAGCAGCAGCA, chromosome 8 at 22,939,648 bp
  • A to G, chromosome 8 at 33,241,101 bp
  • T to A, chromosome 8 at 46,578,910 bp
  • C to T, chromosome 8 at 47,505,304 bp
  • G to A, chromosome 8 at 70,132,003 bp
  • T to C, chromosome 8 at 70,257,107 bp
  • C to T, chromosome 8 at 119,288,721 bp
  • T to C, chromosome 8 at 127,460,386 bp
  • CGG to CG, chromosome 9 at 37,411,490 bp
  • T to C, chromosome 9 at 92,581,323 bp
  • T to C, chromosome 9 at 107,778,014 bp
  • A to C, chromosome 9 at 108,082,215 bp
  • T to C, chromosome 9 at 118,628,205 bp
  • T to A, chromosome 10 at 74,594,183 bp
  • T to C, chromosome 10 at 76,507,539 bp
  • A to G, chromosome 11 at 68,385,712 bp
  • C to T, chromosome 11 at 69,401,642 bp
  • T to C, chromosome 11 at 75,313,344 bp
  • A to T, chromosome 11 at 98,912,262 bp
  • A to T, chromosome 11 at 99,658,713 bp
  • A to G, chromosome 11 at 113,867,031 bp
  • T to A, chromosome 12 at 72,495,594 bp
  • T to A, chromosome 13 at 13,648,802 bp
  • C to T, chromosome 14 at 18,278,332 bp
  • T to A, chromosome 14 at 31,735,006 bp
  • A to G, chromosome 14 at 55,501,571 bp
  • C to A, chromosome 14 at 57,613,409 bp
  • G to T, chromosome 15 at 48,791,944 bp
  • T to A, chromosome 15 at 75,839,590 bp
  • G to T, chromosome 15 at 78,994,391 bp
  • G to A, chromosome 15 at 102,710,985 bp
  • G to A, chromosome 17 at 13,888,952 bp
  • T to A, chromosome 17 at 20,041,884 bp
  • C to A, chromosome 17 at 24,289,414 bp
  • T to A, chromosome 17 at 29,626,706 bp
  • T to C, chromosome 17 at 33,935,948 bp
  • A to G, chromosome 17 at 74,650,047 bp
  • T to G, chromosome 18 at 25,175,431 bp
  • T to C, chromosome 18 at 37,694,721 bp
  • T to A, chromosome 18 at 80,912,858 bp
  • T to C, chromosome 19 at 17,595,658 bp
  • C to T, chromosome 19 at 26,676,726 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5279 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042839-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.