Strain Name:
C57BL/6J-MtgxR5452Btlr/Mmmh
Stock Number:
042848-MU
Citation ID:
RRID:MMRRC_042848-MU
Other Names:
R5452 (G1), C57BL/6J-MtgxR5452Btlr
Major Collection:

Strain Information

Sema3c
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Synonyms: Semae, 1110036B02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20348
Homologene: 36201
Fzd9
Name: frizzled class receptor 9
Synonyms: mfz9, frizzled 9, Fz9
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14371
HGNC: HGNC:4047
Homologene: 2619
Sirt3
Name: sirtuin 3
Synonyms: Sir2l3, 2310003L23Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 64384
Homologene: 81827
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Dhx35
Name: DEAH-box helicase 35
Synonyms: Ddx35, 1200009D07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71715
Homologene: 6406
Psme4
Name: proteasome (prosome, macropain) activator subunit 4
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103554
Homologene: 113742
Map4
Name: microtubule-associated protein 4
Synonyms: MAP 4, Mtap4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17758
HGNC: HGNC:6862
Homologene: 1780
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12695
Homologene: 72199
Tut7
Name: terminal uridylyl transferase 7
Synonyms: 6030448M23Rik, Tent3b, Zcchc6
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 214290
VEGA: 13
Homologene: 51941
Map2k4
Name: mitogen-activated protein kinase kinase 4
Synonyms: MKK4, JNKK1, Serk1, Sek1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 26398
HGNC: HGNC:6844
Homologene: 48159
Xrn2
Name: 5'-3' exoribonuclease 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 24128
Homologene: 6927
Resf1
Name: retroelement silencing factor 1
Synonyms: GET, 2810474O19Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67246
Homologene: 19251
Tjap1
Name: tight junction associated protein 1
Synonyms: 0610041D19Rik, Pilt, Tjp4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 74094
VEGA: 17
Homologene: 12534
Mtrex
Name: Mtr4 exosome RNA helicase
Synonyms: 2610528A15Rik, Skiv2l2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72198
VEGA: 13
Homologene: 6257
Tacc1
Name: transforming, acidic coiled-coil containing protein 1
Synonyms: 4833447E04Rik, B230378H13Rik, Tacc1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 320165
Homologene: 4575
Ccdc80
Name: coiled-coil domain containing 80
Synonyms: Urb, Ssg1, 2610001E17Rik, DRO1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 67896
Homologene: 12206
Ash1l
Name: ASH1 like histone lysine methyltransferase
Synonyms: chromatin remodeling factor, 8030453L17Rik, E430018P19Rik, KMT2H
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 192195
Homologene: 10225
Ttll4
Name: tubulin tyrosine ligase-like family, member 4
Synonyms: 4632407P03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67534
Homologene: 8764
Unc45a
Name: unc-45 myosin chaperone A
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101869
Homologene: 32423
C6
Name: complement component 6
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12274
HGNC: HGNC:1339
Homologene: 47
Hr
Name: lysine demethylase and nuclear receptor corepressor
Synonyms: N, ba, rh, rh-bmh, bldy
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 15460
HGNC: HGNC:5172
Homologene: 3774
Gnb4
Name: guanine nucleotide binding protein (G protein), beta 4
Synonyms: 6720453A21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14696
Homologene: 69140
Calm2
Name: calmodulin 2
Synonyms: 1500001E21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12314
HGNC: HGNC:1445
Homologene: 116117
Dnajc13
Name: DnaJ heat shock protein family (Hsp40) member C13
Synonyms: Rme8, LOC382100, D030002L11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235567
Homologene: 13574
Chkb
Name: choline kinase beta
Synonyms: CK/EK-beta, Chetk, Chkl
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 12651
HGNC: HGNC:1938
Homologene: 88718
Trmt2a
Name: TRM2 tRNA methyltransferase 2A
Synonyms: Htf9c
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 15547
Homologene: 7374
Adam20
Name: a disintegrin and metallopeptidase domain 20
Synonyms: 4930529F22Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 384806
HGNC: HGNC:199
Homologene: 128364
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Ttn
Name: titin
Synonyms: connectin, L56, 1100001C23Rik, mdm, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: D330014H01Rik, 2900022L05Rik, Dnhd3, Dnahc2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Clcn2
Name: chloride channel, voltage-sensitive 2
Synonyms: Clc2, ClC-2, nmf240
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 12724
HGNC: HGNC:2020
Homologene: 3213
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Tnn
Name: tenascin N
Synonyms: Tnw, tenascin-W
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329278
Homologene: 18634
Plcb2
Name: phospholipase C, beta 2
Synonyms: B230205M18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18796
HGNC: HGNC:9055
Homologene: 20957
Prg4
Name: proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein)
Synonyms: MSF, DOL54, lubricin, SZP
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 96875
HGNC: HGNC:9364
Homologene: 137352
Ttc21a
Name: tetratricopeptide repeat domain 21A
Synonyms: 4921538N17Rik, Thm2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74052
Homologene: 14728
Plce1
Name: phospholipase C, epsilon 1
Synonyms: 4933403A21Rik, PLCepsilon
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 74055
Homologene: 9478
Rint1
Name: RAD50 interactor 1
Synonyms: 2810450M21Rik, 1500019C06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72772
Homologene: 11070
Sppl2c
Name: signal peptide peptidase 2C
Synonyms: 4933407P14Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237958
Homologene: 18491
Moxd2
Name: monooxygenase, DBH-like 2
Synonyms: Dbhl1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 194357
Homologene: 77226
Pappa2
Name: pappalysin 2
Synonyms: placenta-specific 3, pregnancy-associated plasma preproprotein-A2, pregnancy-associated plasma protein-E, PAPP-A2, PLAC3, Pappe
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23850
Homologene: 10661
Celsr3
Name: cadherin, EGF LAG seven-pass G-type receptor 3
Synonyms: Fmi1, flamingo, Adgrc3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 107934
HGNC: HGNC:3230
Homologene: 1077
Tnfrsf8
Name: tumor necrosis factor receptor superfamily, member 8
Synonyms: CD30
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 21941
Homologene: 949
Padi2
Name: peptidyl arginine deiminase, type II
Synonyms: PAD type II, Pdi, Pdi2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18600
Homologene: 7214
Crybb3
Name: crystallin, beta B3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12962
HGNC: HGNC:2400
Homologene: 3008
Gemin6
Name: gem nuclear organelle associated protein 6
Synonyms: 2610019B15Rik, 2810470M17Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 67242
Homologene: 11711
Mrgprx1
Name: MAS-related GPR, member X1
Synonyms: MrgC11, Mrgprc11
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 404242
Homologene: 86184
Pcdhb1
Name: protocadherin beta 1
Synonyms: PcdhbA
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93872
HGNC: HGNC:8680
Homologene: 8348
Rsad1
Name: radical S-adenosyl methionine domain containing 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237926
Homologene: 41252
Spam1
Name: sperm adhesion molecule 1
Synonyms: Ph-20
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20690
Homologene: 7547
Or5p68
Name: olfactory receptor family 5 subfamily P member 68
Synonyms: GA_x6K02T2PBJ9-10676998-10676054, MOR204-35, Olfr493
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258307
Homologene: 17188
Tekt3
Name: tektin 3
Synonyms: 4933407G07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71062
Homologene: 12896
Trabd
Name: TraB domain containing
Synonyms: 5730502D15Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 67976
Homologene: 36427
St3gal4
Name: ST3 beta-galactoside alpha-2,3-sialyltransferase 4
Synonyms: ST3Gal IV, Siat4c
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20443
VEGA: 9
Homologene: 4572
Sv2c
Name: synaptic vesicle glycoprotein 2c
Synonyms: 4930527L09Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75209
Homologene: 57152
Adamts5
Name: ADAM metallopeptidase with thrombospondin type 1 motif 5
Synonyms: ADAM-TS5, 9530092O11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 23794
VEGA: 16
HGNC: HGNC:221
Homologene: 5109
Ephb4
Name: Eph receptor B4
Synonyms: MDK2, Myk1, Htk, Tyro11, b2b2412Clo
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13846
HGNC: HGNC:3395
Homologene: 20939
Gm10242
Name: predicted gene 10242
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 100040589
Speer4f2
Name: spermatogenesis associated glutamate (E)-rich protein 4f2
Synonyms: Gm3535, Gm3495
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100041749
Gm9949
Name: predicted gene 9949
Synonyms: EG225609
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225609
VEGA: 18
Srxn1
Name: sulfiredoxin 1 homolog (S. cerevisiae)
Synonyms: 1700127B04Rik, Srx, Npn3, Srx1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 76650
Homologene: 32722
Gm15781
Name: predicted gene 15781
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 108168695
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 74,679,321 bp
  • T to C, chromosome 1 at 150,455,768 bp
  • T to C, chromosome 1 at 158,838,602 bp
  • T to C, chromosome 1 at 160,110,261 bp
  • A to G, chromosome 2 at 40,922,316 bp
  • T to A, chromosome 2 at 76,754,824 bp
  • A to G, chromosome 2 at 118,718,246 bp
  • A to T, chromosome 2 at 147,024,713 bp
  • G to A, chromosome 2 at 152,105,879 bp
  • A to G, chromosome 2 at 158,857,129 bp
  • A to T, chromosome 3 at 32,589,845 bp
  • T to A, chromosome 3 at 88,984,876 bp
  • T to A, chromosome 4 at 98,424,455 bp
  • T to C, chromosome 4 at 140,932,071 bp
  • T to A, chromosome 4 at 145,292,644 bp
  • C to T, chromosome 5 at 17,376,500 bp
  • T to G, chromosome 5 at 17,717,070 bp
  • G to T, chromosome 5 at 23,794,365 bp
  • A to T, chromosome 5 at 113,078,579 bp
  • C to T, chromosome 5 at 135,250,860 bp
  • T to C, chromosome 5 at 137,361,142 bp
  • G to A, chromosome 6 at 24,800,732 bp
  • C to T, chromosome 6 at 40,882,114 bp
  • T to C, chromosome 6 at 48,939,866 bp
  • T to A, chromosome 6 at 149,041,513 bp
  • C to A, chromosome 6 at 149,329,113 bp
  • T to C, chromosome 7 at 48,021,808 bp
  • G to A, chromosome 7 at 80,329,039 bp
  • A to C, chromosome 7 at 108,346,105 bp
  • G to A, chromosome 7 at 140,865,015 bp
  • C to A, chromosome 8 at 25,164,415 bp
  • A to T, chromosome 8 at 40,795,764 bp
  • C to T, chromosome 9 at 35,053,456 bp
  • A to G, chromosome 9 at 104,192,114 bp
  • T to C, chromosome 9 at 108,844,034 bp
  • C to T, chromosome 9 at 110,037,783 bp
  • T to C, chromosome 9 at 119,950,971 bp
  • T to A, chromosome 11 at 30,791,168 bp
  • G to T, chromosome 11 at 63,094,793 bp
  • A to G, chromosome 11 at 65,719,587 bp
  • T to C, chromosome 11 at 69,524,383 bp
  • T to A, chromosome 11 at 94,543,689 bp
  • A to T, chromosome 11 at 104,187,300 bp
  • A to G, chromosome 13 at 59,800,657 bp
  • A to T, chromosome 13 at 95,978,083 bp
  • T to C, chromosome 13 at 112,913,181 bp
  • G to T, chromosome 14 at 70,556,627 bp
  • T to C, chromosome 15 at 4,814,829 bp
  • C to T, chromosome 15 at 89,084,941 bp
  • T to C, chromosome 15 at 89,429,585 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • A to G, chromosome 16 at 15,768,637 bp
  • A to G, chromosome 16 at 18,250,950 bp
  • A to G, chromosome 16 at 20,707,867 bp
  • G to A, chromosome 16 at 45,118,165 bp
  • A to G, chromosome 16 at 85,869,912 bp
  • T to C, chromosome 17 at 46,260,175 bp
  • T to G, chromosome 17 at 80,227,749 bp
  • T to C, chromosome 17 at 87,435,722 bp
  • A to T, chromosome 18 at 37,265,758 bp
  • A to G, chromosome 18 at 62,180,516 bp
  • A to C, chromosome 19 at 38,620,482 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5452 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042848-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.