Strain Name:
C57BL/6J-MtgxR5576Btlr/Mmmh
Stock Number:
043131-MU
Citation ID:
RRID:MMRRC_043131-MU
Other Names:
R5576 (G1), C57BL/6J-MtgxR5576Btlr
Major Collection:

Strain Information

Baiap2
Name: brain-specific angiogenesis inhibitor 1-associated protein 2
Synonyms: IRSp53
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 108100
HGNC: HGNC:947
Homologene: 9697
Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Snx2
Name: sorting nexin 2
Synonyms: 0610030A03Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67804
VEGA: 18
Homologene: 2332
Zfp553
Name: zinc finger protein 553
Synonyms: 2600009K23Rik, C330013F15Rik, ENSMUSG00000054461
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233887
Homologene: 27098
Ankrd17
Name: ankyrin repeat domain 17
Synonyms: Gtar, 4933425K22Rik, A130069E23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81702
Homologene: 82403
Golga4
Name: golgin A4
Synonyms: golgin-245, Olp-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54214
HGNC: HGNC:4427
Homologene: 68224
Nln
Name: neurolysin (metallopeptidase M3 family)
Synonyms: 4930472G13Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75805
VEGA: 13
Homologene: 69315
Xrcc6
Name: X-ray repair complementing defective repair in Chinese hamster cells 6
Synonyms: Ku p70, G22p1, Ku70
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14375
HGNC: HGNC:4055
Homologene: 37483
Sbno2
Name: strawberry notch 2
Synonyms: Stno
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216161
VEGA: 10
Homologene: 8981
Kmt2a
Name: lysine (K)-specific methyltransferase 2A
Synonyms: trithorax Drosophila, ALL-1, Mll1, Cxxc7, Mll, HTRX1, All1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214162
HGNC: HGNC:7132
Homologene: 4338
Agfg1
Name: ArfGAP with FG repeats 1
Synonyms: D730048C23Rik, C130049H11Rik, Rip, Hrb
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 15463
HGNC: HGNC:5175
Homologene: 37929
Zfp26
Name: zinc finger protein 26
Synonyms: Zfp-26, Zfp81-rs1, KRAB15, Zfp70, mkr-3, 5033428C05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22688
Homologene: 52318
Dnaja2
Name: DnaJ heat shock protein family (Hsp40) member A2
Synonyms: 1500017M13Rik, DNJ3, PRO3015, HIRIP4, 2010206B19Rik, mDj3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56445
Homologene: 21193
Melk
Name: maternal embryonic leucine zipper kinase
Synonyms: MPK38
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17279
Homologene: 32111
Apob
Name: apolipoprotein B
Synonyms: apob-48, apob-100
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238055
HGNC: HGNC:603
Homologene: 328
Pole
Name: polymerase (DNA directed), epsilon
Synonyms: pol-epsilon
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18973
HGNC: HGNC:9177
Homologene: 4539
Slc17a8
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 8
Synonyms: Vglut3, Vgt3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216227
Homologene: 13584
4930522L14Rik
Name: RIKEN cDNA 4930522L14 gene
Synonyms: Gm42152
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100041734
Btnl9
Name: butyrophilin-like 9
Synonyms: D330012D11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237754
Homologene: 68540
Rpp30
Name: ribonuclease P/MRP 30 subunit
Synonyms: TSG15, Rnasep2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 54364
VEGA: 19
Homologene: 38180
Snrnp27
Name: small nuclear ribonucleoprotein 27 (U4/U6.U5)
Synonyms: 2610209M04Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66618
Homologene: 135794
Ibtk
Name: inhibitor of Bruton agammaglobulinemia tyrosine kinase
Synonyms: 5430411K16Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 108837
Homologene: 34661
Fads1
Name: fatty acid desaturase 1
Synonyms: 0710001O03Rik, A930006B21Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 76267
VEGA: 19
HGNC: HGNC:3574
Homologene: 22753
Pde4b
Name: phosphodiesterase 4B, cAMP specific
Synonyms: dunce, Dpde4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18578
HGNC: HGNC:8781
Homologene: 1953
Trpc1
Name: transient receptor potential cation channel, subfamily C, member 1
Synonyms: Mtrp1, Trp1, Trrp1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22063
Homologene: 2478
Efcab6
Name: EF-hand calcium binding domain 6
Synonyms: 4932408N08Rik, 4931407K02Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 77627
Homologene: 11259
Agbl1
Name: ATP/GTP binding protein-like 1
Synonyms: Ccp4, EG244071, Nna1-l1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 244071
Homologene: 17552
Add2
Name: adducin 2
Synonyms: 2900072M03Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11519
HGNC: HGNC:244
Homologene: 1221
Fbp2
Name: fructose bisphosphatase 2
Synonyms: FBPase muscle, Fbp-1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 14120
VEGA: 13
HGNC: HGNC:3607
Homologene: 55784
Sycp1
Name: synaptonemal complex protein 1
Synonyms: SCP1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20957
Homologene: 2389
Zan
Name: zonadhesin
Synonyms: Zan
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22635
Homologene: 124417
Ppfia4
Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4
Synonyms: LOC100042382, Gm3812, Liprin-alpha4, 1110008G13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 68507
HGNC: HGNC:9248
Homologene: 66200
BC034090
Name: cDNA sequence BC034090
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 207792
Homologene: 19490
Vmn2r105
Name: vomeronasal 2, receptor 105
Synonyms: EG627743
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 627743
Homologene: 129680
Zkscan6
Name: zinc finger with KRAB and SCAN domains 6
Synonyms: 1700128E15Rik, Zfp535, KOX11, D11Ertd714e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 52712
Homologene: 12119
Nrap
Name: nebulin-related anchoring protein
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18175
HGNC: HGNC:7988
Homologene: 4499
Tada3
Name: transcriptional adaptor 3
Synonyms: 1110004B19Rik, Tada3l, ADA3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 101206
Homologene: 4633
Spata32
Name: spermatogenesis associated 32
Synonyms: 4933400C05Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 328019
Homologene: 17554
Or8b44
Name: olfactory receptor family 8 subfamily B member 44
Synonyms: GA_x6K02T2PVTD-32204729-32205661, MOR165-5, Olfr907
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258801
VEGA: 9
Homologene: 27268
Sbp
Name: spermine binding protein
Synonyms: p25
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20234
Homologene: 117464
Or7g34
Name: olfactory receptor family 7 subfamily G member 34
Synonyms: Olfr854, GA_x6K02T2PVTD-13313295-13312357, MOR147-3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258515
Homologene: 128144
Acaa1a
Name: acetyl-Coenzyme A acyltransferase 1A
Synonyms: peroxisomal 3-ketoacyl-CoA thiolase, thiolase A, D9Ertd25e, PTL, Acaa1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 113868
HGNC: HGNC:82
Homologene: 37497
Ccdc122
Name: coiled-coil domain containing 122
Synonyms: 4933415L06Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 108811
Homologene: 51651
Cybb
Name: cytochrome b-245, beta polypeptide
Synonyms: gp91phox, Nox2, gp91phox, Cgd
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 13058
HGNC: HGNC:2578
Homologene: 68054
Esp15
Name: exocrine gland secreted peptide 15
Synonyms: Gm9041
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 668200
VEGA: 17
Slfn9
Name: schlafen 9
Synonyms: 9830137M10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237886
Homologene: 45432
Bhlha9
Name: basic helix-loop-helix family, member a9
Synonyms: A830053O21Rik, Fingerin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 320522
Homologene: 82363
Trgc2
Name: T cell receptor gamma, constant 2
Synonyms: Trgc2, Gm16603, Tcrg-C2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 107574
Pex11g
Name: peroxisomal biogenesis factor 11 gamma
Synonyms: Pex11c, 1810049N02Rik, Pex11gamma, 1810022F11Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 69129
Homologene: 12298
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 82,870,724 bp
  • T to G, chromosome 1 at 134,323,050 bp
  • T to G, chromosome 1 at 155,241,468 bp
  • T to C, chromosome 3 at 102,818,902 bp
  • A to G, chromosome 4 at 44,312,255 bp
  • T to A, chromosome 4 at 102,430,162 bp
  • A to C, chromosome 5 at 90,243,224 bp
  • G to A, chromosome 5 at 109,737,704 bp
  • A to T, chromosome 5 at 110,312,065 bp
  • A to T, chromosome 5 at 137,428,482 bp
  • T to C, chromosome 6 at 58,888,725 bp
  • G to T, chromosome 6 at 86,107,475 bp
  • T to C, chromosome 6 at 86,682,873 bp
  • T to C, chromosome 6 at 113,374,794 bp
  • C to T, chromosome 7 at 76,335,237 bp
  • C to T, chromosome 7 at 127,236,703 bp
  • A to G, chromosome 8 at 3,465,875 bp
  • A to T, chromosome 8 at 85,539,404 bp
  • T to A, chromosome 9 at 19,567,073 bp
  • A to G, chromosome 9 at 20,437,507 bp
  • A to C, chromosome 9 at 38,498,908 bp
  • A to G, chromosome 9 at 44,842,634 bp
  • C to T, chromosome 9 at 85,732,718 bp
  • A to G, chromosome 9 at 95,721,324 bp
  • A to G, chromosome 9 at 118,553,534 bp
  • T to A, chromosome 9 at 119,344,569 bp
  • C to T, chromosome 10 at 80,067,337 bp
  • A to G, chromosome 10 at 89,597,502 bp
  • T to C, chromosome 11 at 49,178,885 bp
  • T to C, chromosome 11 at 65,834,096 bp
  • T to C, chromosome 11 at 76,672,769 bp
  • A to T, chromosome 11 at 82,981,432 bp
  • T to A, chromosome 11 at 103,209,827 bp
  • T to A, chromosome 11 at 119,996,911 bp
  • A to T, chromosome 12 at 7,998,662 bp
  • T to C, chromosome 13 at 19,305,131 bp
  • A to T, chromosome 13 at 62,837,191 bp
  • A to G, chromosome 13 at 104,058,830 bp
  • G to A, chromosome 14 at 77,091,877 bp
  • T to A, chromosome 15 at 82,022,492 bp
  • C to A, chromosome 15 at 83,950,000 bp
  • C to T, chromosome 17 at 20,224,574 bp
  • T to A, chromosome 17 at 23,945,578 bp
  • C to A, chromosome 17 at 39,642,673 bp
  • A to G, chromosome 18 at 53,210,750 bp
  • A to G, chromosome 19 at 10,185,874 bp
  • T to A, chromosome 19 at 36,101,851 bp
  • C to T, chromosome 19 at 56,321,982 bp
  • C to G, chromosome X at 9,450,750 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5576 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043131-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.