Strain Name:
C57BL/6J-MtgxR6654Btlr/Mmmh
Stock Number:
044775-MU
Citation ID:
RRID:MMRRC_044775-MU
Other Names:
R6654 (G1)
Major Collection:

Strain Information

Prkacb
Name: protein kinase, cAMP dependent, catalytic, beta
Synonyms: Pkacb, cAMP-dependent protein kinase C beta
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18749
HGNC: HGNC:9381
Homologene: 121718
Gsta4
Name: glutathione S-transferase, alpha 4
Synonyms: mGsta4, GST 5.7
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14860
VEGA: 9
Homologene: 135605
Ddc
Name: dopa decarboxylase
Synonyms: Aadc, aromatic L-amino acid decarboxylase
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13195
HGNC: HGNC:2719
Homologene: 618
Armc6
Name: armadillo repeat containing 6
Synonyms: 2410153K17Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 76813
Homologene: 14121
Kif16b
Name: kinesin family member 16B
Synonyms: N-3 kinesin, 8430434E15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16558
Homologene: 135708
Exd1
Name: exonuclease 3'-5' domain containing 1
Synonyms: 4932702D22Rik, Exdl1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241624
Homologene: 17627
Msh3
Name: mutS homolog 3
Synonyms: D13Em1, Rep3, Rep-3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17686
HGNC: HGNC:7326
Homologene: 1829
Sorl1
Name: sortilin-related receptor, LDLR class A repeats-containing
Synonyms: Sorla, LR11, mSorLA, 2900010L19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20660
Homologene: 2336
Mfng
Name: MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms: manic fringe
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17305
HGNC: HGNC:7038
Homologene: 1803
Nbas
Name: neuroblastoma amplified sequence
Synonyms: 4933425L03Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 71169
VEGA: 12
Homologene: 41073
Bhlhe40
Name: basic helix-loop-helix family, member e40
Synonyms: eip1 (E47 interaction protein 1), DEC1, cytokine response gene 8, Stra13, CR8, Bhlhb2, Clast5, C130042M06Rik, Sharp2, Stra14
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 20893
HGNC: HGNC:1046
Homologene: 2722
Nlrc4
Name: NLR family, CARD domain containing 4
Synonyms: Ipaf, Card12, 9530011P19Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 268973
VEGA: 17
Homologene: 10924
Rpgrip1l
Name: Rpgrip1-like
Synonyms: 1700047E16Rik, Nphp8, fantom, Ftm
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244585
Homologene: 18296
Med12l
Name: mediator complex subunit 12-like
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 329650
Homologene: 43143
Pkd1l3
Name: polycystic kidney disease 1 like 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244646
Homologene: 14627
Gm4847
Name: predicted gene 4847
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226604
Homologene: 101040
Myo7b
Name: myosin VIIB
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 17922
HGNC: HGNC:7607
Homologene: 81947
Unc79
Name: unc-79 homolog
Synonyms: 9030205A07Rik, Mlca3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217843
Homologene: 41397
Vmn2r111
Name: vomeronasal 2, receptor 111
Synonyms: EG210876
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210876
Homologene: 86604
Nubpl
Name: nucleotide binding protein-like
Synonyms: 2410170E07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 76826
Homologene: 11854
Irs2
Name: insulin receptor substrate 2
Synonyms: Irs-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 384783
HGNC: HGNC:6126
Homologene: 2778
Rsph4a
Name: radial spoke head 4 homolog A (Chlamydomonas)
Synonyms: Rshl3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 212892
Homologene: 71779
Vmn2r112
Name: vomeronasal 2, receptor 112
Synonyms: EG628185
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 628185
Homologene: 86604
Zfp974
Name: zinc finger protein 974
Synonyms: 1700049G17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 73430
Tmem39b
Name: transmembrane protein 39b
Synonyms: 6330509E05Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230770
Homologene: 23069
Or5aq6
Name: olfactory receptor family 5 subfamily AQ member 6
Synonyms: GA_x6K02T2Q125-48586461-48585523, MOR172-5, Olfr1109
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258762
Homologene: 86672
Akr1b1
Name: aldo-keto reductase family 1 member B
Synonyms: Aldr1, Aldor1, Akr1b3, ALR2, Ahr-1, AR, Ahr1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 11677
HGNC: HGNC:381
Homologene: 133743
Potefam1
Name: POTE ankyrin domain family member 1
Synonyms: Potea, Pote1, A26c3, 4930430A15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67575
Homologene: 69410
Zfp850
Name: zinc finger protein 850
Synonyms: C130069I09Rik, Gm4636
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100043772
P2ry12
Name: purinergic receptor P2Y, G-protein coupled 12
Synonyms: P2Y12, 4921504D23Rik, 2900079B22Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 70839
Homologene: 11260
Gm5431
Name: predicted gene 5431
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 432555
Gm15922
Name: predicted gene 15922
Type: Gene
Species: Mouse
Chromosome: 7
Krtap12-1
Name: keratin associated protein 12-1
Synonyms: D10Jhu14e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16694
VEGA: 10
Homologene: 135687
Gm13757
Name: predicted gene 13757
Type: Gene
Species: Mouse
Chromosome: 2
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 166,630,387 bp
  • A to G, chromosome 2 at 87,093,050 bp
  • T to G, chromosome 2 at 88,446,672 bp
  • T to A, chromosome 2 at 111,171,884 bp
  • T to A, chromosome 2 at 119,524,717 bp
  • T to C, chromosome 2 at 142,701,277 bp
  • A to G, chromosome 3 at 59,218,020 bp
  • G to T, chromosome 3 at 59,262,292 bp
  • A to G, chromosome 3 at 146,750,543 bp
  • A to T, chromosome 4 at 129,686,826 bp
  • C to T, chromosome 6 at 34,310,004 bp
  • TG to TGG, chromosome 6 at 108,664,857 bp
  • A to T, chromosome 7 at 3,735,929 bp
  • A to G, chromosome 7 at 27,926,403 bp
  • A to T, chromosome 7 at 27,985,215 bp
  • A to G, chromosome 8 at 11,006,486 bp
  • T to C, chromosome 8 at 70,231,375 bp
  • T to C, chromosome 8 at 91,220,205 bp
  • T to G, chromosome 8 at 109,624,283 bp
  • T to C, chromosome 9 at 41,980,645 bp
  • T to C, chromosome 9 at 78,209,099 bp
  • A to G, chromosome 10 at 33,912,992 bp
  • T to C, chromosome 10 at 77,720,703 bp
  • A to T, chromosome 11 at 11,880,452 bp
  • T to C, chromosome 11 at 48,894,600 bp
  • C to T, chromosome 12 at 13,483,874 bp
  • T to A, chromosome 12 at 52,310,733 bp
  • A to C, chromosome 12 at 103,079,048 bp
  • A to T, chromosome 12 at 103,079,049 bp
  • T to C, chromosome 13 at 92,345,042 bp
  • G to A, chromosome 14 at 47,690,000 bp
  • T to A, chromosome 15 at 78,759,339 bp
  • T to C, chromosome 17 at 22,559,051 bp
  • G to T, chromosome 17 at 22,603,469 bp
  • G to A, chromosome 17 at 74,445,528 bp
  • T to C, chromosome 18 at 31,990,269 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6654 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044775-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.