Strain Name:
C57BL/6J-MtgxR8174Btlr/Mmmh
Stock Number:
067599-MU
Citation ID:
RRID:MMRRC_067599-MU
Other Names:
R8174 (G1)
Major Collection:

Strain Information

Slc6a5
Name: solute carrier family 6 (neurotransmitter transporter, glycine), member 5
Synonyms: Glyt2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 104245
Homologene: 37901
Syt4
Name: synaptotagmin IV
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 20983
VEGA: 18
Homologene: 7559
Strn4
Name: striatin, calmodulin binding protein 4
Synonyms: ZIN, zinedin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 97387
Homologene: 8378
Akap13
Name: A kinase anchor protein 13
Synonyms: 5730522G15Rik, 1700026G02Rik, PROTO-LB, Ht31, PROTO-LBC, 5830460E08Rik, AKAP-Lbc
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 75547
HGNC: HGNC:371
Homologene: 4903
Ppp1r12a
Name: protein phosphatase 1, regulatory subunit 12A
Synonyms: Mypt1, 5730577I22Rik, D10Ertd625e, 1200015F06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17931
VEGA: 10
HGNC: HGNC:7618
Homologene: 1855
Srrm2
Name: serine/arginine repetitive matrix 2
Synonyms: SRm300, 5033413A03Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 75956
Rspry1
Name: ring finger and SPRY domain containing 1
Synonyms: 4930470D19Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 67610
Homologene: 12164
Polg
Name: polymerase (DNA directed), gamma
Synonyms: polymerase gamma, Polga, mitochondrial DNA polymerase gamma, mitochondrial DNA polymerase-gamma, Pol gamma
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18975
HGNC: HGNC:9179
Homologene: 2016
Slc43a2
Name: solute carrier family 43, member 2
Synonyms: 7630402D21Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 215113
Homologene: 17555
Erap1
Name: endoplasmic reticulum aminopeptidase 1
Synonyms: Arts1, ERAAP, PILSAP
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 80898
VEGA: 13
Homologene: 56754
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: Coh1, 2310042E16Rik, 1810042B05Rik, C330002D13Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Calm1
Name: calmodulin 1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 12313
HGNC: HGNC:1442
Homologene: 116117
Zfp266
Name: zinc finger protein 266
Synonyms: 5730601F06Rik, 5330440G10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 77519
Homologene: 105676
Cachd1
Name: cache domain containing 1
Synonyms: Vwcd1, 1190007F10Rik, B430218L07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320508
Homologene: 10854
Asxl3
Name: ASXL transcriptional regulator 3
Synonyms: D430002O22Rik, LOC381127, D930044O18Rik, C230079D11Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 211961
Homologene: 19371
Mpc2
Name: mitochondrial pyruvate carrier 2
Synonyms: 0610006C01Rik, 2610205H19Rik, ESTM43, 2010002I07Rik, Brp44
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 70456
Homologene: 31675
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: Dnahc5, Mdnah5, b2b1134Clo, b2b1154Clo, b2b1537Clo, b2b1565Clo, b2b3491Clo
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Pcnx4
Name: pecanex homolog 4
Synonyms: Pcnxl4, 1810048J11Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 67708
VEGA: 12
Homologene: 23366
Cdsn
Name: corneodesmosin
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 386463
HGNC: HGNC:1802
Rev3l
Name: REV3 like, DNA directed polymerase zeta catalytic subunit
Synonyms: Sez4, Rev
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19714
HGNC: HGNC:9968
Homologene: 48147
Ddx60
Name: DExD/H box helicase 60
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234311
Homologene: 23031
Phlpp2
Name: PH domain and leucine rich repeat protein phosphatase 2
Synonyms: Phlppl, C130044A18Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244650
Homologene: 71015
Tpsg1
Name: tryptase gamma 1
Synonyms: TMT, Prss31
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26945
Homologene: 74994
Zcwpw1
Name: zinc finger, CW type with PWWP domain 1
Synonyms: LOC381678
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 381678
Homologene: 9944
Matn2
Name: matrilin 2
Synonyms: Crtm2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17181
VEGA: 15
HGNC: HGNC:6908
Homologene: 20538
Tfb1m
Name: transcription factor B1, mitochondrial
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224481
VEGA: 17
Homologene: 9343
Sp140l1
Name: Sp140 nuclear body protein like 1
Synonyms: LOC381287, A530032D15Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381287
Homologene: 131208
Rnf43
Name: ring finger protein 43
Synonyms: 4732452J19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 207742
Homologene: 37742
Ppp2r3a
Name: protein phosphatase 2, regulatory subunit B'', alpha
Synonyms: 3222402P14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235542
HGNC: HGNC:9307
Homologene: 20595
Veph1
Name: ventricular zone expressed PH domain-containing 1
Synonyms: 2810471M23Rik, Veph
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 72789
Homologene: 11624
Gm2042
Name: predicted gene 2042
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 100039087
Homologene: 103830
Zmym5
Name: zinc finger, MYM-type 5
Synonyms: 9830124H08Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 219105
Homologene: 16997
Or5b12b
Name: olfactory receptor family 5 subfamily B member 12B
Synonyms: GA_x6K02T2RE5P-3213352-3214296, MOR202-7, Olfr1445
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258694
Homologene: 133606
Rap2a
Name: RAS related protein 2a
Synonyms: 5830461H18Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 76108
HGNC: HGNC:9861
Homologene: 10875
Or7d10
Name: olfactory receptor family 7 subfamily D member 10
Synonyms: GA_x6K02T2PVTD-13660026-13660964, Olfr77, 18A, MOR143-1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258336
HGNC: HGNC:8380
Homologene: 133688
Dlg2
Name: discs large MAGUK scaffold protein 2
Synonyms: Gm21505, A330103J02Rik, PSD93, Chapsyn-110, B330007M19Rik, B230218P12Rik, Dlgh2, LOC382816
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23859
HGNC: HGNC:2901
Homologene: 1046
Dnajc28
Name: DnaJ heat shock protein family (Hsp40) member C28
Synonyms: ORF28
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 246738
HGNC: HGNC:1297
Homologene: 9869
Rabl3
Name: RAB, member RAS oncogene family-like 3
Synonyms: 4930553C05Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 67657
Homologene: 12171
Tram1l1
Name: translocation associated membrane protein 1-like 1
Synonyms: A830091N21Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229801
Homologene: 17142
Cldn11
Name: claudin 11
Synonyms: Otm, oligodendrocyte-specific protein, Osp
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18417
HGNC: HGNC:8514
Homologene: 4093
Pkmyt1
Name: protein kinase, membrane associated tyrosine/threonine 1
Synonyms: Myt1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 268930
VEGA: 17
Homologene: 31227
Fam179b
Name:
Type: Gene
Species: Mouse
Chromosome: 12
Gm8797
Name: predicted pseudogene 8797
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 667759
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 85,099,540 bp
  • T to A, chromosome 1 at 165,480,889 bp
  • G to A, chromosome 2 at 175,169,675 bp
  • T to C, chromosome 3 at 5,751,034 bp
  • T to C, chromosome 3 at 31,154,061 bp
  • A to G, chromosome 3 at 66,263,895 bp
  • A to G, chromosome 3 at 124,322,262 bp
  • T to A, chromosome 4 at 100,966,269 bp
  • G to A, chromosome 5 at 137,819,577 bp
  • C to T, chromosome 7 at 16,828,808 bp
  • A to G, chromosome 7 at 49,948,309 bp
  • A to G, chromosome 7 at 75,728,869 bp
  • A to T, chromosome 7 at 79,456,718 bp
  • A to G, chromosome 7 at 91,940,040 bp
  • G to A, chromosome 8 at 62,017,250 bp
  • T to A, chromosome 8 at 94,649,822 bp
  • G to A, chromosome 8 at 109,868,689 bp
  • A to C, chromosome 9 at 19,920,724 bp
  • G to A, chromosome 9 at 20,506,814 bp
  • T to C, chromosome 9 at 101,213,302 bp
  • T to C, chromosome 10 at 39,859,115 bp
  • G to T, chromosome 10 at 108,271,737 bp
  • T to G, chromosome 11 at 75,543,367 bp
  • G to T, chromosome 11 at 87,731,231 bp
  • G to T, chromosome 12 at 64,982,691 bp
  • T to A, chromosome 12 at 72,556,913 bp
  • T to A, chromosome 12 at 87,960,158 bp
  • G to T, chromosome 12 at 100,205,708 bp
  • A to T, chromosome 13 at 74,646,564 bp
  • T to A, chromosome 14 at 56,794,461 bp
  • T to A, chromosome 14 at 120,478,857 bp
  • A to G, chromosome 15 at 28,311,133 bp
  • C to A, chromosome 15 at 34,422,409 bp
  • C to G, chromosome 15 at 35,709,310 bp
  • T to C, chromosome 16 at 37,556,863 bp
  • G to A, chromosome 16 at 91,616,907 bp
  • T to C, chromosome 17 at 3,519,580 bp
  • A to T, chromosome 17 at 23,733,839 bp
  • C to T, chromosome 17 at 23,815,323 bp
  • T to C, chromosome 17 at 25,372,590 bp
  • A to T, chromosome 17 at 35,555,632 bp
  • T to C, chromosome 18 at 22,517,743 bp
  • T to A, chromosome 18 at 31,444,177 bp
  • A to G, chromosome 19 at 12,883,904 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8174 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067599-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.