Strain Name:
C57BL/6J-MtgxR8499Btlr/Mmmh
Stock Number:
067941-MU
Citation ID:
RRID:MMRRC_067941-MU
Other Names:
R8499 (G1)
Major Collection:

Strain Information

Hgf
Name: hepatocyte growth factor
Synonyms: scatter factor, NK2, NK1, C230052L06Rik, HGF/SF, SF/HGF
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15234
HGNC: HGNC:4893
Homologene: 503
Ltbp3
Name: latent transforming growth factor beta binding protein 3
Synonyms: Ltbp2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16998
VEGA: 19
HGNC: HGNC:6716
Homologene: 7405
Lcmt1
Name: leucine carboxyl methyltransferase 1
Synonyms: Lcmt, LCMT-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 30949
Homologene: 41123
Prkci
Name: protein kinase C, iota
Synonyms: aPKClambda, 2310021H13Rik, PKClambda, Pkci, Prkcl, Pkcl
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18759
HGNC: HGNC:9404
Homologene: 37667
Eif4g3
Name: eukaryotic translation initiation factor 4 gamma, 3
Synonyms: G1-419-52, 4930523M17Rik, repro8, 1500002J22Rik, eIF4GII
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230861
HGNC: HGNC:3298
Homologene: 2789
Nacc1
Name: nucleus accumbens associated 1, BEN and BTB (POZ) domain containing
Synonyms: 2010001H03Rik, Nac1, 4930511N13Rik, Btbd14b
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66830
VEGA: 8
Homologene: 12042
Ythdf3
Name: YTH N6-methyladenosine RNA binding protein 3
Synonyms: 9130022A11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229096
Homologene: 34991
Tubgcp5
Name: tubulin, gamma complex component 5
Synonyms: GCP5, B130010C12Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233276
Homologene: 14172
Nedd4l
Name: neural precursor cell expressed, developmentally down-regulated gene 4-like
Synonyms: Nedd4b, Nedd4-2, 1300012C07Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 83814
VEGA: 18
HGNC: HGNC:7728
Homologene: 86986
Cast
Name: calpastatin
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 12380
VEGA: 13
HGNC: HGNC:1515
Homologene: 7658
Sh3glb2
Name: SH3-domain GRB2-like endophilin B2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227700
Homologene: 10595
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: C330002D13Rik, 2310042E16Rik, Coh1, 1810042B05Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Grhl3
Name: grainyhead like transcription factor 3
Synonyms: ct, Get1, Som
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230824
Homologene: 18864
Bhmt
Name: betaine-homocysteine methyltransferase
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 12116
HGNC: HGNC:1047
Homologene: 1295
Clcn1
Name: chloride channel, voltage-sensitive 1
Synonyms: nmf355, Clc1, SMCC1, NMF355, Clc-1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12723
HGNC: HGNC:2019
Homologene: 63
Ttn
Name: titin
Synonyms: mdm, D830007G01Rik, shru, 1100001C23Rik, D330041I19Rik, 2310057K23Rik, 2310036G12Rik, 2310074I15Rik, L56, connectin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Tnfsf14
Name: tumor necrosis factor (ligand) superfamily, member 14
Synonyms: LIGHT, HVEM-L
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 50930
VEGA: 17
Homologene: 2822
Oc90
Name: otoconin 90
Synonyms: Pla2ll, Ocn-95, PLA2L
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 18256
HGNC: HGNC:8100
Homologene: 19021
Vmn2r61
Name: vomeronasal 2, receptor 61
Synonyms: EG637873, Gprc2a-rs2, Casr-rs2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 637873
Homologene: 129683
Vmn1r59
Name: vomeronasal 1 receptor 59
Synonyms: V1rd10
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 404284
Homologene: 41799
Ap4b1
Name: adaptor-related protein complex AP-4, beta 1
Synonyms: 1810038H16Rik, AP-4 beta-4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67489
HGNC: HGNC:572
Homologene: 38203
Zfp1001
Name: zinc finger protein 1001
Synonyms: Gm10130
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 102633750
VEGA: 2
Dynap
Name: dynactin associated protein
Synonyms: 2310002L13Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 75577
VEGA: 18
Homologene: 82463
Atp11b
Name: ATPase, class VI, type 11B
Synonyms: 1110019I14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76295
Homologene: 32919
Slc22a5
Name: solute carrier family 22 (organic cation transporter), member 5
Synonyms: Octn2, Lstpl
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20520
Homologene: 68295
Adam25
Name: ADAM metallopeptidase domain 25
Synonyms: testase 2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 23793
HGNC: HGNC:199
Homologene: 128364
Fbll1
Name: fibrillarin-like 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237730
Homologene: 28185
Pappa2
Name: pappalysin 2
Synonyms: placenta-specific 3, pregnancy-associated plasma protein-E, pregnancy-associated plasma preproprotein-A2, Pappe, PLAC3, PAPP-A2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23850
Homologene: 10661
Grk4
Name: G protein-coupled receptor kinase 4
Synonyms: Gprk2l, A830025H08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14772
HGNC: HGNC:4543
Homologene: 23158
Atf6b
Name: activating transcription factor 6 beta
Synonyms: ATF6beta, Crebl1, Creb-rp
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12915
HGNC: HGNC:2349
Homologene: 31238
Apol7c
Name: apolipoprotein L 7c
Synonyms: 2210421G13Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 108956
VEGA: 15
HGNC: HGNC:619
Homologene: 40940
Card14
Name: caspase recruitment domain family, member 14
Synonyms: CARMA2, Bimp2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 170720
Homologene: 11469
Or52i2
Name: olfactory receptor family 52 subfamily J member 2
Synonyms: MOR41-1, Olfr556, GA_x6K02T2PBJ9-5386601-5387575
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258749
Homologene: 17380
Tmem63c
Name: transmembrane protein 63c
Synonyms: 9330187M14Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217733
Homologene: 33129
Qpct
Name: glutaminyl-peptide cyclotransferase (glutaminyl cyclase)
Synonyms: 5730422A13Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 70536
VEGA: 17
HGNC: HGNC:9753
Homologene: 8238
Pygm
Name: muscle glycogen phosphorylase
Synonyms: PG
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 19309
HGNC: HGNC:9726
Homologene: 2145
Pou2f2
Name: POU domain, class 2, transcription factor 2
Synonyms: Oct2d, Otf2, Oct2b, Oct2a, Oct-2, Otf-2, Oct2c
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18987
HGNC: HGNC:9213
Homologene: 55674
Cnot6l
Name: CCR4-NOT transcription complex, subunit 6-like
Synonyms: 4932442K20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231464
Homologene: 100830
Fbxw22
Name: F-box and WD-40 domain protein 22
Synonyms: Gm5164
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 382156
Homologene: 110776
Usp16
Name: ubiquitin specific peptidase 16
Synonyms: 6330514E22Rik, UBP-M, 1200004E02Rik, 2810483I07Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 74112
Homologene: 38183
Synpo
Name: synaptopodin
Synonyms: 9030217H17Rik, 9330140I15Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 104027
Homologene: 5274
Or51a25
Name: olfactory receptor family 51 subfamily A member 25
Synonyms: GA_x6K02T2PBJ9-5441154-5440198, MOR11-1, Olfr559
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259116
Homologene: 74124
Snai3
Name: snail family zinc finger 3
Synonyms: Zfp293, Smuc, Snail-related gene from muscle cells
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 30927
Homologene: 8500
Exoc8
Name: exocyst complex component 8
Synonyms: SEC84, Exo84p, EXO84
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102058
Homologene: 44545
Klk1b22
Name: kallikrein 1-related peptidase b22
Synonyms: mGk-22, Egfbp-1, Egfbp1, Klk22
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13646
Homologene: 68141
Btbd7
Name: BTB domain containing 7
Synonyms: FUP1, E130118E17Rik, 5730507E09Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238386
VEGA: 12
Homologene: 34300
Lrrc36
Name: leucine rich repeat containing 36
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 270091
Homologene: 23090
Ifi211
Name: interferon activated gene 211
Synonyms: Mnda, Ifi205b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381308
Homologene: 115929
Fxyd1
Name: FXYD domain-containing ion transport regulator 1
Synonyms: 0610012C17Rik, 1110006M24Rik, phospholemman, PML, PLM
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56188
HGNC: HGNC:4025
Homologene: 3691
H2bc8
Name: H2B clustered histone 8
Synonyms: Hist1h2bg
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 319181
Homologene: 136774
H2-Q5
Name: histocompatibility 2, Q region locus 5
Synonyms: Qa5, H-2Q5, Qa-5, Qat-5
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15016
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 158,936,522 bp
  • T to C, chromosome 1 at 173,905,520 bp
  • A to T, chromosome 2 at 30,359,204 bp
  • A to G, chromosome 2 at 76,918,991 bp
  • T to C, chromosome 2 at 150,362,987 bp
  • A to G, chromosome 3 at 16,205,015 bp
  • A to G, chromosome 3 at 31,025,217 bp
  • G to T, chromosome 3 at 35,810,705 bp
  • G to A, chromosome 3 at 103,820,702 bp
  • C to A, chromosome 4 at 135,549,238 bp
  • A to C, chromosome 4 at 138,165,928 bp
  • A to T, chromosome 5 at 16,566,856 bp
  • A to G, chromosome 5 at 34,745,346 bp
  • A to T, chromosome 5 at 96,077,317 bp
  • A to G, chromosome 6 at 42,307,199 bp
  • C to T, chromosome 7 at 5,454,751 bp
  • C to A, chromosome 7 at 25,100,198 bp
  • C to A, chromosome 7 at 31,053,104 bp
  • T to A, chromosome 7 at 42,300,276 bp
  • T to C, chromosome 7 at 44,112,720 bp
  • T to A, chromosome 7 at 55,804,615 bp
  • T to A, chromosome 7 at 102,670,805 bp
  • A to G, chromosome 7 at 102,723,725 bp
  • C to A, chromosome 7 at 123,430,148 bp
  • A to G, chromosome 8 at 40,755,152 bp
  • A to T, chromosome 8 at 84,676,716 bp
  • A to T, chromosome 8 at 105,449,536 bp
  • G to A, chromosome 8 at 122,456,405 bp
  • A to G, chromosome 8 at 124,897,110 bp
  • A to T, chromosome 9 at 109,385,000 bp
  • C to T, chromosome 11 at 35,798,080 bp
  • A to G, chromosome 11 at 53,867,643 bp
  • T to C, chromosome 11 at 119,331,244 bp
  • C to T, chromosome 12 at 87,072,964 bp
  • A to T, chromosome 12 at 102,788,372 bp
  • T to A, chromosome 13 at 23,571,706 bp
  • A to T, chromosome 13 at 74,798,716 bp
  • T to A, chromosome 13 at 93,620,092 bp
  • T to C, chromosome 15 at 35,841,320 bp
  • C to T, chromosome 15 at 65,881,556 bp
  • G to T, chromosome 15 at 77,526,080 bp
  • C to T, chromosome 16 at 87,474,648 bp
  • T to C, chromosome 17 at 34,650,822 bp
  • A to G, chromosome 17 at 35,394,844 bp
  • C to T, chromosome 17 at 35,394,969 bp
  • A to G, chromosome 17 at 57,190,534 bp
  • A to G, chromosome 17 at 79,077,567 bp
  • T to C, chromosome 18 at 60,602,972 bp
  • T to A, chromosome 18 at 65,209,657 bp
  • T to A, chromosome 18 at 70,240,973 bp
  • T to A, chromosome 19 at 5,748,684 bp
  • A to G, chromosome 19 at 6,390,362 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8499 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067941-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.