Strain Name:
C57BL/6J-MtgxR8548Btlr/Mmmh
Stock Number:
068513-MU
Citation ID:
RRID:MMRRC_068513-MU
Other Names:
R8548 (G1)
Major Collection:

Strain Information

Usp7
Name: ubiquitin specific peptidase 7
Synonyms: 2210010O09Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 252870
Homologene: 2592
Plxnd1
Name: plexin D1
Synonyms: 6230425C21Rik, b2b1863Clo, b2b553Clo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67784
HGNC: HGNC:9107
Homologene: 22866
Nmt1
Name: N-myristoyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18107
HGNC: HGNC:7857
Homologene: 69027
Usp32
Name: ubiquitin specific peptidase 32
Synonyms: 2900074J03Rik, 6430526O11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237898
Homologene: 13066
Odf2
Name: outer dense fiber of sperm tails 2
Synonyms: MMTEST29, cenexin
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18286
HGNC: HGNC:8114
Homologene: 1907
Srfbp1
Name: serum response factor binding protein 1
Synonyms: 2810036K01Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67222
VEGA: 18
Homologene: 12101
Ints9
Name: integrator complex subunit 9
Synonyms: D14Ertd231e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 210925
VEGA: 14
Homologene: 10096
Dmbx1
Name: diencephalon/mesencephalon homeobox 1
Synonyms: Dmbx1, Cdmx, Mbx, Atx, Otx3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 140477
Homologene: 15639
Dmac2
Name: distal membrane arm assembly complex 2
Synonyms: 2310004L02Rik, Atp5sl, C030044E10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 66349
Homologene: 9973
Cftr
Name: cystic fibrosis transmembrane conductance regulator
Synonyms: Abcc7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12638
HGNC: HGNC:1884
Homologene: 55465
Map2
Name: microtubule-associated protein 2
Synonyms: MAP-2, G1-397-34, repro4, Mtap2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17756
HGNC: HGNC:6839
Homologene: 1779
Eloa
Name: elongin A
Synonyms: Tceb3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 27224
Homologene: 37746
Nr6a1
Name: nuclear receptor subfamily 6, group A, member 1
Synonyms: 1700113M01Rik, NCNF, Gcnf
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14536
HGNC: HGNC:7985
Homologene: 36308
Cdc20
Name: cell division cycle 20
Synonyms: p55CDC, 2310042N09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 107995
HGNC: HGNC:1723
Homologene: 37459
Ilkap
Name: integrin-linked kinase-associated serine/threonine phosphatase 2C
Synonyms: 0710007A14Rik, PP2C-DELTA, 1600009O09Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67444
Homologene: 31525
Sp8
Name: trans-acting transcription factor 8
Synonyms: mBtd, D930049B17Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320145
VEGA: 12
Homologene: 18548
Skor2
Name: SKI family transcriptional corepressor 2
Synonyms: Fussel18, Corl2, Gm7348
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 664805
VEGA: 18
Homologene: 64797
Ern2
Name: endoplasmic reticulum to nucleus signalling 2
Synonyms: Ire1b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26918
Homologene: 22687
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Pico, Acz
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 26875
Homologene: 69111
Fam135b
Name: family with sequence similarity 135, member B
Synonyms: 1700010C24Rik, A830008O07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70363
VEGA: 15
Homologene: 66605
Stxbp5
Name: syntaxin binding protein 5 (tomosyn)
Synonyms: 4930565N16Rik, LGL3, 0710001E20Rik, tomosyn 1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 78808
Homologene: 16402
Macc1
Name: metastasis associated in colon cancer 1
Synonyms: 4732474O15Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238455
Homologene: 18813
Rflnb
Name: refilin B
Synonyms: RefilinB, Fam101b, cfm, 1500005K14Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76566
Homologene: 18809
Bsn
Name: bassoon
Synonyms: presynaptic cytomatrix protein
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12217
HGNC: HGNC:1117
Homologene: 31161
Osbpl6
Name: oxysterol binding protein-like 6
Synonyms: 1110062M20Rik, ORP-6
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99031
Homologene: 101447
Hoxa2
Name: homeobox A2
Synonyms: Hox-1.11
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 15399
HGNC: HGNC:5103
Homologene: 4901
Mapkbp1
Name: mitogen-activated protein kinase binding protein 1
Synonyms: 2810483F24Rik, Jnkbp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 26390
Homologene: 69109
Fbxl6
Name: F-box and leucine-rich repeat protein 6
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 30840
VEGA: 15
Homologene: 8130
Prss23
Name: serine protease 23
Synonyms: 2310046G15Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76453
Homologene: 5191
Mgat5
Name: mannoside acetylglucosaminyltransferase 5
Synonyms: GlcNAc-TV, beta1,6N-acetylglucosaminyltransferase V, 4930471A21Rik, 5330407H02Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 107895
HGNC: HGNC:7049
Homologene: 1808
Prdm11
Name: PR domain containing 11
Synonyms: 8030443D09Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 100042784
Homologene: 82511
Or8g37
Name: olfactory receptor family 8 subfamily G member 37
Synonyms: MOR171-16, GA_x6K02T2PVTD-33517322-33518257, Olfr970
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258604
VEGA: 9
Homologene: 121532
Thnsl1
Name: threonine synthase-like 1 (bacterial)
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 208967
Homologene: 32609
Hspa8
Name: heat shock protein 8
Synonyms: Hsc73, 2410008N15Rik, Hsc71, Hsc70, Hsp73
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 15481
HGNC: HGNC:5241
Homologene: 68524
Apoa2
Name: apolipoprotein A-II
Synonyms: Hdl-1, Alp-2, Apoa-2, ApoA-II
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11807
HGNC: HGNC:601
Homologene: 1242
Myoz2
Name: myozenin 2
Synonyms: 1110012I24Rik, Fatz-2, calsarcin-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 59006
HGNC: HGNC:1330
Homologene: 9583
Adam1a
Name: a disintegrin and metallopeptidase domain 1a
Synonyms: fertilin alpha, PH-30 alpha, Ftna
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 280668
HGNC: HGNC:187
Homologene: 73803
Akirin1
Name: akirin 1
Synonyms: 6330407G11Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68050
Homologene: 11348
Gpr171
Name: G protein-coupled receptor 171
Synonyms: F730001G15Rik, H963
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229323
Homologene: 36330
Ap5b1
Name: adaptor-related protein complex 5, beta 1 subunit
Synonyms: Gm962
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 381201
VEGA: 19
Homologene: 16299
Ctf1
Name: cardiotrophin 1
Synonyms: CT-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13019
HGNC: HGNC:2499
Homologene: 1016
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 66,413,340 bp
  • G to T, chromosome 1 at 91,391,160 bp
  • G to A, chromosome 1 at 127,320,672 bp
  • T to A, chromosome 1 at 171,226,229 bp
  • A to G, chromosome 2 at 21,212,922 bp
  • T to C, chromosome 2 at 29,893,514 bp
  • C to A, chromosome 2 at 38,729,538 bp
  • T to G, chromosome 2 at 38,729,539 bp
  • A to G, chromosome 2 at 76,579,222 bp
  • C to T, chromosome 2 at 93,012,758 bp
  • A to G, chromosome 2 at 120,024,091 bp
  • A to T, chromosome 3 at 59,097,979 bp
  • T to A, chromosome 3 at 123,034,267 bp
  • A to T, chromosome 4 at 115,920,315 bp
  • A to C, chromosome 4 at 118,436,338 bp
  • A to G, chromosome 4 at 123,738,038 bp
  • T to C, chromosome 4 at 136,005,677 bp
  • A to G, chromosome 5 at 14,682,254 bp
  • A to T, chromosome 5 at 121,520,102 bp
  • G to T, chromosome 6 at 18,273,699 bp
  • G to A, chromosome 6 at 52,163,118 bp
  • C to T, chromosome 6 at 115,957,597 bp
  • T to A, chromosome 7 at 25,624,792 bp
  • A to G, chromosome 7 at 89,510,208 bp
  • G to A, chromosome 7 at 122,177,839 bp
  • A to T, chromosome 7 at 127,717,392 bp
  • T to A, chromosome 9 at 39,820,241 bp
  • A to G, chromosome 9 at 40,802,471 bp
  • G to A, chromosome 9 at 108,111,452 bp
  • C to T, chromosome 10 at 9,817,306 bp
  • A to G, chromosome 11 at 76,022,221 bp
  • G to A, chromosome 11 at 85,017,827 bp
  • A to G, chromosome 11 at 103,043,226 bp
  • A to C, chromosome 12 at 118,849,175 bp
  • T to A, chromosome 12 at 119,450,356 bp
  • T to C, chromosome 14 at 65,032,321 bp
  • T to C, chromosome 15 at 71,462,810 bp
  • T to A, chromosome 15 at 76,537,342 bp
  • A to T, chromosome 16 at 8,712,075 bp
  • G to A, chromosome 18 at 52,488,391 bp
  • T to A, chromosome 18 at 76,858,886 bp
  • G to T, chromosome 19 at 5,571,095 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8548 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068513-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.