Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR0158Btlr/Mmmh
Stock Number:
038438-MU
Citation ID:
RRID:MMRRC_038438-MU
Other Names:
R0158 (G1), C57BL/6J-MtgxR0158Btlr
Major Collection:

Strain Information

Cdkn2a
Name: cyclin dependent kinase inhibitor 2A
Synonyms: MTS1, p19ARF, p16INK4a, INK4a-ARF, p16, ARF-INK4a, Arf, Pctr1, p19ARF, Ink4a/Arf
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12578
HGNC: HGNC:1787
Homologene: 55430
Vps54
Name: VPS54 GARP complex subunit
Synonyms: Vps54l, 5330404P15Rik, mSLP8, wr
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 245944
Homologene: 5605
Jak2
Name: Janus kinase 2
Synonyms: C81284
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16452
VEGA: 19
HGNC: HGNC:6192
Homologene: 21033
Rgs3
Name: regulator of G-protein signaling 3
Synonyms: 4930506N09Rik, C2pa, PDZ-RGS3, RGS3S, C2PA-RGS3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 50780
HGNC: HGNC:9999
Homologene: 32440
Ulk1
Name: unc-51 like kinase 1
Synonyms: Unc51.1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22241
Homologene: 2640
Kcnc4
Name: potassium voltage gated channel, Shaw-related subfamily, member 4
Synonyms: Kv3.4, Kcr2-4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99738
HGNC: HGNC:6236
Homologene: 68427
Nudt5
Name: nudix hydrolase 5
Synonyms: nudix (nucleoside diphosphate linked moiety X)-type motif 5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 53893
HGNC: HGNC:8052
Homologene: 5388
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 13,152,384 bp
  • T to G, chromosome 1 at 26,683,951 bp
  • G to A, chromosome 2 at 5,862,303 bp
  • T to C, chromosome 2 at 10,234,992 bp
  • C to A, chromosome 2 at 83,792,037 bp
  • T to C, chromosome 2 at 152,932,410 bp
  • T to A, chromosome 2 at 181,997,413 bp
  • T to C, chromosome 3 at 84,210,169 bp
  • T to A, chromosome 3 at 103,091,730 bp
  • C to A, chromosome 3 at 107,458,604 bp
  • A to G, chromosome 4 at 6,379,042 bp
  • A to G, chromosome 4 at 57,709,649 bp
  • A to T, chromosome 4 at 62,623,884 bp
  • T to C, chromosome 4 at 63,729,992 bp
  • A to T, chromosome 4 at 89,276,767 bp
  • A to T, chromosome 4 at 113,184,814 bp
  • A to T, chromosome 4 at 141,042,242 bp
  • A to G, chromosome 4 at 141,200,329 bp
  • C to A, chromosome 5 at 16,361,817 bp
  • A to T, chromosome 5 at 96,776,634 bp
  • T to C, chromosome 5 at 100,092,889 bp
  • A to T, chromosome 5 at 110,788,944 bp
  • T to C, chromosome 5 at 117,217,242 bp
  • C to A, chromosome 5 at 118,742,449 bp
  • T to A, chromosome 5 at 150,051,628 bp
  • T to C, chromosome 6 at 115,924,484 bp
  • C to T, chromosome 6 at 119,236,748 bp
  • A to G, chromosome 7 at 28,155,492 bp
  • C to T, chromosome 7 at 102,713,955 bp
  • G to A, chromosome 8 at 22,327,739 bp
  • T to A, chromosome 8 at 71,537,217 bp
  • G to A, chromosome 8 at 88,250,743 bp
  • T to C, chromosome 8 at 93,219,429 bp
  • T to G, chromosome 8 at 106,913,386 bp
  • T to C, chromosome 8 at 109,855,635 bp
  • C to T, chromosome 9 at 40,208,916 bp
  • T to A, chromosome 9 at 66,495,921 bp
  • A to T, chromosome 9 at 78,688,932 bp
  • C to T, chromosome 9 at 96,686,940 bp
  • T to G, chromosome 9 at 108,464,137 bp
  • A to G, chromosome 9 at 109,654,652 bp
  • C to T, chromosome 9 at 121,750,691 bp
  • T to C, chromosome 10 at 103,389,347 bp
  • G to A, chromosome 10 at 127,033,273 bp
  • A to G, chromosome 10 at 128,438,235 bp
  • A to T, chromosome 11 at 21,306,962 bp
  • C to T, chromosome 11 at 46,143,034 bp
  • G to T, chromosome 11 at 55,296,185 bp
  • T to C, chromosome 11 at 69,940,319 bp
  • T to C, chromosome 11 at 116,295,292 bp
  • A to G, chromosome 12 at 72,556,302 bp
  • A to T, chromosome 12 at 91,740,602 bp
  • A to G, chromosome 12 at 104,217,008 bp
  • T to A, chromosome 13 at 22,219,628 bp
  • T to A, chromosome 13 at 23,752,710 bp
  • A to G, chromosome 13 at 54,524,717 bp
  • T to A, chromosome 13 at 113,369,153 bp
  • G to A, chromosome 14 at 50,964,304 bp
  • T to C, chromosome 14 at 70,521,447 bp
  • A to G, chromosome 15 at 58,898,878 bp
  • T to C, chromosome 16 at 3,715,456 bp
  • C to T, chromosome 16 at 20,552,566 bp
  • C to T, chromosome 16 at 64,855,838 bp
  • C to T, chromosome 16 at 89,793,001 bp
  • G to A, chromosome 17 at 21,819,819 bp
  • A to G, chromosome 17 at 57,224,851 bp
  • G to A, chromosome 17 at 57,244,381 bp
  • A to G, chromosome 18 at 10,647,840 bp
  • A to C, chromosome 18 at 37,297,230 bp
  • C to A, chromosome 19 at 29,311,757 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0158 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038438-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.