Strain Name:
C57BL/6J-MtgxR1447Btlr/Mmmh
Stock Number:
039502-MU
Citation ID:
RRID:MMRRC_039502-MU
Other Names:
R1447 (G1), C57BL/6J-MtgxR1447Btlr
Major Collection:

Gene Information

Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20269
Homologene: 56005
Fubp3
Name: far upstream element (FUSE) binding protein 3
Synonyms: FBP3, A330051M14Rik, Marta2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320267
HGNC: HGNC:4005
Homologene: 45954
Usp47
Name: ubiquitin specific peptidase 47
Synonyms: 4930502N04Rik, A630020C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74996
Homologene: 9929
Mast2
Name: microtubule associated serine/threonine kinase 2
Synonyms: MAST205, Mtssk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17776
Homologene: 7428
Enpp2
Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
Synonyms: Autotaxin, Npps2, PD-Ialpha, Pdnp2, ATX
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18606
HGNC: HGNC:3357
Homologene: 4526
Uhmk1
Name: U2AF homology motif (UHM) kinase 1
Synonyms: KIS, Kist, OTTMUSG00000021542, C820018A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16589
Homologene: 7801
Etaa1
Name: Ewing tumor-associated antigen 1
Synonyms: 5730466H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68145
Homologene: 10369
Zfp532
Name: zinc finger protein 532
Synonyms: C530030I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 328977
Homologene: 138627
Bnc2
Name: basonuclin 2
Synonyms: 5031434M05Rik, 8430420F16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242509
Homologene: 18243
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107182
Homologene: 31978
Zfhx3
Name: zinc finger homeobox 3
Synonyms: WBP9, A230102L03Rik, Atbf1, Sci
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11906
HGNC: HGNC:777
Homologene: 21366
Nup205
Name: nucleoporin 205
Synonyms: 3830404O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 70699
Homologene: 45971
Opa1
Name: OPA1, mitochondrial dynamin like GTPase
Synonyms: 1200011N24Rik, lilr3, optic atrophy 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74143
HGNC: HGNC:8140
Homologene: 14618
4932438A13Rik
Name: RIKEN cDNA 4932438A13 gene
Synonyms: Tweek, FSA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229227
Homologene: 52105
Rps6ka5
Name: ribosomal protein S6 kinase, polypeptide 5
Synonyms: 6330404E13Rik, MSK1, 3110005L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 73086
VEGA: 12
Homologene: 48302
Mphosph10
Name: M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein)
Synonyms: 2810453H10Rik, 5730405D16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67973
HGNC: HGNC:7213
Homologene: 134741
Amotl1
Name: angiomotin-like 1
Synonyms: 4932416D09Rik, JEAP, 2310067L22Rik, 2310010G08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 75723
Homologene: 43977
Utp15
Name: UTP15 small subunit processome component
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105372
VEGA: 13
Homologene: 6629
Lama5
Name: laminin, alpha 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16776
HGNC: HGNC:6485
Homologene: 4060
Rpl7
Name: ribosomal protein L7
Synonyms: Rpl7a, Surf-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19989
Homologene: 87772
Atp6v1b1
Name: ATPase, H+ transporting, lysosomal V1 subunit B1
Synonyms: Vpp3, Vpp-3, Atp6b1, lysosomal 56/58kDa, D630039P21Rik, D630030L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 110935
HGNC: HGNC:853
Homologene: 68198
Phf24
Name: PHD finger protein 24
Synonyms: N28178, GINIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230085
Homologene: 18208
Adamts12
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239337
VEGA: 15
Homologene: 12808
C1qtnf3
Name: C1q and tumor necrosis factor related protein 3
Synonyms: CORS-26, Corcs, 2310005P21Rik, CTRP3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 81799
Homologene: 12788
Ddx24
Name: DEAD box helicase 24
Synonyms: 1700055J08Rik, 2510027P10Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 27225
VEGA: 12
Homologene: 10702
Golga2
Name: golgi autoantigen, golgin subfamily a, 2
Synonyms: GM130
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99412
HGNC: HGNC:4425
Homologene: 3300
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 94109
Homologene: 69536
Dnah1
Name: dynein, axonemal, heavy chain 1
Synonyms: MDHC7, E030034C22Rik, B230373P09Rik, Dnahc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 110084
VEGA: 14
HGNC: HGNC:2940
Homologene: 67131
Smarcc2
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
Synonyms: 5930405J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 68094
VEGA: 10
Homologene: 2312
Zfpl1
Name: zinc finger like protein 1
Synonyms: 1500015B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 81909
Homologene: 4935
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: hy-3, hy3, 1700034M11Rik, 4930545D19Rik, hyrh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244653
Homologene: 52118
Tmprss7
Name: transmembrane serine protease 7
Synonyms: LOC385645, B230219I23Rik, matriptase-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 208171
Homologene: 18178
Myh13
Name: myosin, heavy polypeptide 13, skeletal muscle
Synonyms: extraocular myosin, EO Myosin, MyHC-eo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 544791
HGNC: HGNC:7571
Homologene: 55780
Eps8l1
Name: EPS8-like 1
Synonyms: 4632407K17Rik, EPS8R1, DRC3, 2310051G19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67425
Homologene: 15767
Cyp2j7
Name: cytochrome P450, family 2, subfamily j, polypeptide 7
Synonyms: OTTMUSG00000007941, Cyp2j7-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 546837
Dnah9
Name: dynein, axonemal, heavy chain 9
Synonyms: D11Ertd686e, Dnahc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237806
HGNC: HGNC:2953
Homologene: 20357
Serpinb6d
Name: serine (or cysteine) peptidase inhibitor, clade B, member 6d
Synonyms: SPI3D, Gm11390
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 238568
HGNC: HGNC:8950
Haus5
Name: HAUS augmin-like complex, subunit 5
Synonyms: 2310022K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 71909
Homologene: 18969
Ankrd50
Name: ankyrin repeat domain 50
Synonyms: E430012K20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99696
Homologene: 129859
Ros1
Name: Ros1 proto-oncogene
Synonyms: Ros-1, c-ros
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19886
Homologene: 2207
Ahnak
Name: AHNAK nucleoprotein (desmoyokin)
Synonyms: DY6, 1110004P15Rik, 2310047C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 66395
VEGA: 19
HGNC: HGNC:347
Homologene: 67425
Pik3r5
Name: phosphoinositide-3-kinase regulatory subunit 5
Synonyms: Foap2, p101
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 320207
Homologene: 8627
Rida
Name: reactive intermediate imine deaminase A homolog
Synonyms: HRP12, HR12, Hrsp12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 15473
Homologene: 4261
Aoc1
Name: amine oxidase, copper-containing 1
Synonyms: 1600012D06Rik, Abp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 76507
HGNC: HGNC:80
Homologene: 68159
Olfr356
Name: olfactory receptor 356
Synonyms: GA_x6K02T2NLDC-33631647-33632594, MOR134-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258617
Homologene: 85948
Sh3rf2
Name: SH3 domain containing ring finger 2
Synonyms: RNF158, 9130023G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 269016
Homologene: 17618
Sdcbp
Name: syndecan binding protein
Synonyms: syntenin, syndecan interacting protein, Sycl, MDA-9, syntenin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 53378
Homologene: 4110
Zfp976
Name: zinc finger protein 976
Synonyms: 9830147E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 208111
Homologene: 134004
Tmprss13
Name: transmembrane protease, serine 13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214531
Homologene: 12936
Smarca5
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
Synonyms: Snf2h, D330027N15Rik, 4933427E24Rik, D030040M08Rik, MommeD4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 93762
Homologene: 55764
Thsd4
Name: thrombospondin, type I, domain containing 4
Synonyms: B230114P05Rik, ADAMTSL6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 207596
Homologene: 49796
Nlrp5
Name: NLR family, pyrin domain containing 5
Synonyms: Op1, Mater, Nalp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 23968
Homologene: 65105
Thoc2l
Name: THO complex subunit 2-like
Synonyms: Gm3179, BC005561
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100042165
Olfr380
Name: olfactory receptor 380
Synonyms: GA_x6K02T2P1NL-3613021-3612086, MOR135-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259027
Homologene: 74109
Tmed3
Name: transmembrane p24 trafficking protein 3
Synonyms: 1200002G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66111
Homologene: 7213
Vmn1r35
Name: vomeronasal 1 receptor 35
Synonyms: V1rc12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171185
Homologene: 110800
Gm9934
Name: predicted gene 9934
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Aif1
Name: allograft inflammatory factor 1
Synonyms: G1, Iba1, D17H6S50E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 11629
HGNC: HGNC:352
Homologene: 1226
Fam71f1
Name: family with sequence similarity 71, member F1
Synonyms: LOC330277
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330277
Homologene: 13077
Ccdc162
Name: coiled-coil domain containing 162
Synonyms: 5033413D22Rik, Gm29096, Gm6976
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 75973
Homologene: 136355
Atp8b2
Name: ATPase, class I, type 8B, member 2
Synonyms: Id
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 54667
Homologene: 23226
Tssc4
Name: tumor-suppressing subchromosomal transferable fragment 4
Synonyms: ESTM671070
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56844
Homologene: 4169
Mterf3
Name: mitochondrial transcription termination factor 3
Synonyms: 2410017I18Rik, Mterfd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66410
Homologene: 9310
Plaat3
Name: phospholipase A and acyltransferase 3
Synonyms: Hrasls3, Pla2g16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 225845
Homologene: 5136
Ppp1r42
Name: protein phosphatase 1, regulatory subunit 42
Synonyms: 1700011J18Rik, 4930418G15Rik, Lrrc67
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 69312
Homologene: 27077
Mapre3
Name: microtubule-associated protein, RP/EB family, member 3
Synonyms: EB3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100732
HGNC: HGNC:6892
Homologene: 56565
Eef1akmt1
Name: EEF1A alpha lysine methyltransferase 1
Synonyms: GtAyu21-96, Ayu21-96, Gt(Ayu21)96Imeg, 2510005D08Rik, N6amt2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 68043
VEGA: 14
Homologene: 5952
Tssk5
Name: testis-specific serine kinase 5
Synonyms: 1700091F14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 73542
VEGA: 15
Homologene: 18832
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 9,976,581 bp
  • T to A, chromosome 1 at 16,102,597 bp
  • T to C, chromosome 1 at 170,208,645 bp
  • T to C, chromosome 2 at 31,600,547 bp
  • T to C, chromosome 2 at 32,297,776 bp
  • T to G, chromosome 2 at 36,937,776 bp
  • A to T, chromosome 2 at 65,469,980 bp
  • A to C, chromosome 2 at 180,185,878 bp
  • T to C, chromosome 3 at 36,965,586 bp
  • A to T, chromosome 3 at 38,455,542 bp
  • A to T, chromosome 3 at 89,944,170 bp
  • T to C, chromosome 4 at 6,394,507 bp
  • G to T, chromosome 4 at 42,938,232 bp
  • A to G, chromosome 4 at 84,293,220 bp
  • A to T, chromosome 4 at 96,195,293 bp
  • C to A, chromosome 4 at 116,312,013 bp
  • C to T, chromosome 5 at 30,861,807 bp
  • T to C, chromosome 5 at 104,522,204 bp
  • G to A, chromosome 6 at 29,334,174 bp
  • A to G, chromosome 6 at 35,215,185 bp
  • G to A, chromosome 6 at 48,906,242 bp
  • A to C, chromosome 6 at 66,678,906 bp
  • T to C, chromosome 6 at 83,757,942 bp
  • A to G, chromosome 7 at 4,474,056 bp
  • A to G, chromosome 7 at 23,421,362 bp
  • G to T, chromosome 7 at 30,661,791 bp
  • G to T, chromosome 7 at 42,612,599 bp
  • A to T, chromosome 7 at 64,380,950 bp
  • C to T, chromosome 7 at 93,080,160 bp
  • G to T, chromosome 7 at 112,074,568 bp
  • A to G, chromosome 7 at 143,070,155 bp
  • C to A, chromosome 8 at 15,925,306 bp
  • T to C, chromosome 8 at 80,730,081 bp
  • A to C, chromosome 8 at 108,948,444 bp
  • T to C, chromosome 8 at 110,523,166 bp
  • A to G, chromosome 9 at 14,555,742 bp
  • C to A, chromosome 9 at 45,328,580 bp
  • T to C, chromosome 9 at 59,997,213 bp
  • A to G, chromosome 9 at 89,703,024 bp
  • T to C, chromosome 10 at 41,580,247 bp
  • T to C, chromosome 10 at 52,098,858 bp
  • T to C, chromosome 10 at 128,469,791 bp
  • A to T, chromosome 11 at 17,946,625 bp
  • A to T, chromosome 11 at 66,108,482 bp
  • A to G, chromosome 11 at 67,362,574 bp
  • G to A, chromosome 11 at 68,494,177 bp
  • A to G, chromosome 11 at 73,453,874 bp
  • A to G, chromosome 12 at 100,577,825 bp
  • T to C, chromosome 12 at 103,424,307 bp
  • A to G, chromosome 13 at 33,670,756 bp
  • A to G, chromosome 13 at 66,917,039 bp
  • A to G, chromosome 13 at 98,252,878 bp
  • A to T, chromosome 14 at 31,306,898 bp
  • T to A, chromosome 14 at 57,565,984 bp
  • G to A, chromosome 15 at 10,952,649 bp
  • A to T, chromosome 15 at 11,263,361 bp
  • T to A, chromosome 15 at 34,488,611 bp
  • C to T, chromosome 15 at 54,919,598 bp
  • G to A, chromosome 15 at 76,372,104 bp
  • T to C, chromosome 16 at 29,610,659 bp
  • T to C, chromosome 16 at 45,680,670 bp
  • G to A, chromosome 17 at 35,172,151 bp
  • T to A, chromosome 18 at 42,101,671 bp
  • C to A, chromosome 18 at 65,624,990 bp
  • A to G, chromosome 19 at 6,082,619 bp
  • G to A, chromosome 19 at 7,579,233 bp
  • A to T, chromosome 19 at 9,007,082 bp
  • A to G, chromosome 19 at 36,992,454 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1447 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039502-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.