Strain Name:
C57BL/6J-MtgxR1548Btlr/Mmmh
Stock Number:
039587-MU
Citation ID:
RRID:MMRRC_039587-MU
Other Names:
R1548 (G1), C57BL/6J-MtgxR1548Btlr
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Ints6
Name: integrator complex subunit 6
Synonyms: Notch2l, 2900075H24Rik, DICE1, Ddx26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 18130
VEGA: 14
Homologene: 8121
Ubap2
Name: ubiquitin-associated protein 2
Synonyms: 1190005K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 68926
Homologene: 73649
Foxp1
Name: forkhead box P1
Synonyms: 4932443N09Rik, 3110052D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 108655
HGNC: HGNC:3823
Homologene: 13092
Gpr19
Name: G protein-coupled receptor 19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14760
HGNC: HGNC:4473
Homologene: 4476
Ddx10
Name: DEAD box helicase 10
Synonyms: 4632415A01Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 77591
VEGA: 9
HGNC: HGNC:2735
Homologene: 20922
Gpr21
Name: G protein-coupled receptor 21
Synonyms: C230004C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 338346
HGNC: HGNC:4476
Homologene: 74546
Nek6
Name: NIMA (never in mitosis gene a)-related expressed kinase 6
Synonyms: 1300007C09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 59126
HGNC: HGNC:7749
Homologene: 49379
Grhl2
Name: grainyhead like transcription factor 2
Synonyms: BOM, 0610015A08Rik, Tcfcp2l3, grainyheadlike, clft3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 252973
HGNC: HGNC:2799
Homologene: 32616
Bnc2
Name: basonuclin 2
Synonyms: 5031434M05Rik, 8430420F16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242509
Homologene: 18243
Ddx4
Name: DEAD box helicase 4
Synonyms: mvh / m'vasa, VASA, Mvh, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 13206
VEGA: 13
Homologene: 49227
Ddx27
Name: DEAD box helicase 27
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228889
Homologene: 6431
Cct8
Name: chaperonin containing Tcp1, subunit 8 (theta)
Synonyms: Cctq, Tcpq
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12469
HGNC: HGNC:1623
Homologene: 4802
Ago3
Name: argonaute RISC catalytic subunit 3
Synonyms: argonaute 3, eIF2C3, C130014L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 214150
Homologene: 49799
Mon2
Name: MON2 homolog, regulator of endosome to Golgi trafficking
Synonyms: SF21, 2610528O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67074
VEGA: 10
Homologene: 44309
Scaper
Name: S phase cyclin A-associated protein in the ER
Synonyms: D530014O03Rik, Zfp291
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244891
VEGA: 9
Homologene: 32488
Lrp6
Name: low density lipoprotein receptor-related protein 6
Synonyms: skam26Jus, Cd, ska26, skax26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16974
HGNC: HGNC:6698
Homologene: 1747
Xdh
Name: xanthine dehydrogenase
Synonyms: Xox-1, Xox1, Xor, xanthine oxidase, XO
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22436
VEGA: 17
Homologene: 324
Tshr
Name: thyroid stimulating hormone receptor
Synonyms: hyt, pet, hypothroid
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 22095
Homologene: 315
Tmem245
Name: transmembrane protein 245
Synonyms: A630051L19Rik, D730040F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242474
HGNC: HGNC:1363
Homologene: 41841
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Rassf1
Name: Ras association (RalGDS/AF-6) domain family member 1
Synonyms: 123F protein, REH3P21, NORE2A, RDA32, Rassf1C, Rassf1B, Rassf1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56289
HGNC: HGNC:9882
Homologene: 10499
Drd3
Name: dopamine receptor D3
Synonyms: D3R, D3 receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 13490
VEGA: 16
HGNC: HGNC:3024
Homologene: 623
Olfml1
Name: olfactomedin-like 1
Synonyms: MVAL564, mONT2, ONT2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244198
Homologene: 45688
E2f4
Name: E2F transcription factor 4
Synonyms: 2010111M04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 104394
HGNC: HGNC:3118
Homologene: 1471
Fmnl2
Name: formin-like 2
Synonyms: 5430425K04Rik, man
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71409
Homologene: 70871
Zfp142
Name: zinc finger protein 142
Synonyms: 9330177B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 77264
Homologene: 3723
Pigt
Name: phosphatidylinositol glycan anchor biosynthesis, class T
Synonyms: CGI-06, 4930534E15Rik, Ndap7, NDAP, 2510012P17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 78928
Homologene: 6134
Myo15a
Name: myosin XVA
Synonyms: Myo15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17910
HGNC: HGNC:7594
Homologene: 56504
Cib1
Name: calcium and integrin binding 1 (calmyrin)
Synonyms: Kip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 23991
Homologene: 4654
Cfap74
Name: cilia and flagella associated protein 74
Synonyms: 2010015L04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 544678
Homologene: 129584
Acad10
Name: acyl-Coenzyme A dehydrogenase family, member 10
Synonyms: 2410021P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71985
Homologene: 49825
Cpa1
Name: carboxypeptidase A1, pancreatic
Synonyms: 0910001L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 109697
HGNC: HGNC:2296
Homologene: 55609
Muc6
Name: mucin 6, gastric
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 353328
HGNC: HGNC:7517
Homologene: 18768
Klra3
Name: killer cell lectin-like receptor, subfamily A, member 3
Synonyms: 5E6, Ly49C, Nk-2, Ly49c, NK-2.1, Nk2.1, Nk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16634
Homologene: 110821
Rnf213
Name: ring finger protein 213
Synonyms: D11Ertd759e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 672511
Homologene: 45439
Lrp12
Name: low density lipoprotein-related protein 12
Synonyms: C820005L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239393
Homologene: 8385
Mgam
Name: maltase-glucoamylase
Synonyms: 6030407P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232714
HGNC: HGNC:7043
Homologene: 130099
Ugcg
Name: UDP-glucose ceramide glucosyltransferase
Synonyms: GlcT-1, Epcs21, Ugcgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22234
Homologene: 37763
Ttf1
Name: transcription termination factor, RNA polymerase I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22130
Homologene: 137223
Cacna1s
Name: calcium channel, voltage-dependent, L type, alpha 1S subunit
Synonyms: Cchl1a3, fmd, mdg, sj, muscle dysgenesis, Cav1.1, DHPR alpha1s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12292
HGNC: HGNC:1397
Homologene: 37257
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239420
Homologene: 65982
Spata6
Name: spermatogenesis associated 6
Synonyms: 1700062C23Rik, Hash, KRP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67946
Homologene: 10410
Rgl3
Name: ral guanine nucleotide dissociation stimulator-like 3
Synonyms: 1300003D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 71746
VEGA: 9
Homologene: 11363
Pfkfb2
Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Synonyms: PFK-2/FBPase-2 gene B, 4930568D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18640
HGNC: HGNC:8873
Homologene: 88554
AC132131.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Ifi47
Name: interferon gamma inducible protein 47
Synonyms: 47kDa, IRG-47, Iigp4, Igrd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15953
Homologene: 49169
Nwd2
Name: NACHT and WD repeat domain containing 2
Synonyms: B830017A01Rik, 3110047P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 319807
Homologene: 14974
Ppm1d
Name: protein phosphatase 1D magnesium-dependent, delta isoform
Synonyms: Wip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 53892
HGNC: HGNC:9277
Homologene: 31185
Plxnb1
Name: plexin B1
Synonyms: 2900002G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235611
HGNC: HGNC:9103
Homologene: 130508
Notch4
Name: notch 4
Synonyms: Int-3, Int3, N4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18132
HGNC: HGNC:7884
Homologene: 3351
Lgals12
Name: lectin, galactose binding, soluble 12
Synonyms: galectin-related inhibitor of proliferation, GRIP1, galectin-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56072
Homologene: 10462
Gm5771
Name: predicted gene 5771
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 436523
HGNC: HGNC:9486
Homologene: 88408
Ankfn1
Name: ankyrin-repeat and fibronectin type III domain containing 1
Synonyms: LOC382543, 4932411E22Rik, nmf9, mWAKE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 382543
Homologene: 34996
Hif3a
Name: hypoxia inducible factor 3, alpha subunit
Synonyms: MOP7, bHLHe17, Nepas
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 53417
Homologene: 9646
Ang2
Name: angiogenin, ribonuclease A family, member 2
Synonyms: Rnase5b, Angrp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 11731
VEGA: 14
HGNC: HGNC:483
A830018L16Rik
Name: RIKEN cDNA A830018L16 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 320492
Homologene: 14194
Meis2
Name: Meis homeobox 2
Synonyms: Meis2, Stra10, A430109D20Rik, Mrg1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17536
HGNC: HGNC:7001
Homologene: 7846
Pabpc1l
Name: poly(A) binding protein, cytoplasmic 1-like
Synonyms: ePAB, 1810053B01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 381404
Homologene: 77989
Itga3
Name: integrin alpha 3
Synonyms: VLA-3 alpha 3, alpha3-integrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16400
HGNC: HGNC:6139
Homologene: 21129
Igdcc4
Name: immunoglobulin superfamily, DCC subclass, member 4
Synonyms: 9330155G14Rik, WI-18508, Nope, WI-16786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56741
VEGA: 9
Homologene: 10570
Hoxb4
Name: homeobox B4
Synonyms: Hox-2.6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15412
HGNC: HGNC:5115
Homologene: 32095
Bcl2l12
Name: BCL2-like 12 (proline rich)
Synonyms: Bcl2-L12, 2810475P17Rik, 5430429M05Rik, Bcl-L12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75736
Homologene: 12619
Tcirg1
Name: T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3
Synonyms: V-ATPase a3, OC-116, TIRC7, ATP6a3, Atp6i
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 27060
Homologene: 4392
Ftdc1
Name: ferritin domain containing 1
Synonyms: LOC328695, LOC385656, Gm813
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 328695
VEGA: 16
2610203C20Rik
Name: RIKEN cDNA 2610203C20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Gm10229
Name: predicted gene 10229
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 100040201
VEGA: 16
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 11,518,594 bp
  • G to T, chromosome 1 at 74,570,104 bp
  • G to T, chromosome 1 at 130,698,083 bp
  • T to C, chromosome 1 at 136,110,937 bp
  • A to C, chromosome 2 at 29,065,138 bp
  • C to T, chromosome 2 at 37,518,072 bp
  • T to A, chromosome 2 at 38,568,895 bp
  • A to G, chromosome 2 at 53,105,537 bp
  • C to T, chromosome 2 at 116,058,702 bp
  • G to T, chromosome 2 at 164,037,171 bp
  • C to T, chromosome 2 at 164,501,519 bp
  • G to A, chromosome 2 at 167,034,464 bp
  • C to T, chromosome 4 at 41,199,872 bp
  • A to T, chromosome 4 at 56,906,233 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • A to G, chromosome 4 at 84,275,957 bp
  • T to C, chromosome 4 at 111,779,006 bp
  • T to A, chromosome 4 at 126,370,395 bp
  • C to T, chromosome 4 at 155,434,045 bp
  • A to T, chromosome 5 at 63,800,182 bp
  • G to T, chromosome 5 at 121,626,040 bp
  • G to C, chromosome 5 at 121,626,041 bp
  • G to A, chromosome 6 at 30,642,335 bp
  • T to C, chromosome 6 at 40,750,990 bp
  • G to T, chromosome 6 at 41,396,011 bp
  • A to T, chromosome 6 at 98,945,420 bp
  • G to C, chromosome 6 at 130,333,144 bp
  • G to A, chromosome 6 at 134,459,429 bp
  • A to G, chromosome 6 at 134,870,084 bp
  • T to C, chromosome 7 at 17,044,403 bp
  • C to G, chromosome 7 at 44,992,818 bp
  • A to T, chromosome 7 at 80,228,414 bp
  • T to C, chromosome 7 at 107,590,375 bp
  • G to T, chromosome 7 at 141,638,772 bp
  • A to G, chromosome 7 at 141,652,103 bp
  • A to G, chromosome 8 at 105,304,688 bp
  • G to T, chromosome 9 at 21,980,706 bp
  • T to C, chromosome 9 at 41,581,376 bp
  • T to C, chromosome 9 at 53,149,561 bp
  • C to T, chromosome 9 at 55,816,670 bp
  • T to C, chromosome 9 at 65,135,227 bp
  • A to T, chromosome 9 at 75,171,746 bp
  • C to A, chromosome 9 at 107,551,846 bp
  • C to T, chromosome 9 at 109,100,900 bp
  • T to C, chromosome 10 at 90,049,985 bp
  • C to T, chromosome 10 at 123,036,007 bp
  • A to G, chromosome 11 at 49,095,871 bp
  • A to G, chromosome 11 at 60,488,238 bp
  • C to T, chromosome 11 at 85,339,605 bp
  • T to C, chromosome 11 at 89,526,541 bp
  • A to G, chromosome 11 at 95,046,919 bp
  • C to T, chromosome 11 at 96,318,899 bp
  • G to A, chromosome 11 at 119,442,707 bp
  • T to C, chromosome 12 at 91,534,031 bp
  • A to T, chromosome 13 at 11,554,549 bp
  • T to C, chromosome 13 at 112,599,997 bp
  • C to A, chromosome 14 at 51,195,533 bp
  • G to A, chromosome 14 at 62,713,692 bp
  • C to T, chromosome 15 at 37,336,323 bp
  • A to G, chromosome 15 at 39,872,506 bp
  • A to G, chromosome 15 at 47,981,975 bp
  • A to G, chromosome 16 at 43,821,341 bp
  • A to T, chromosome 16 at 58,615,839 bp
  • A to G, chromosome 16 at 87,485,584 bp
  • G to A, chromosome 16 at 89,015,389 bp
  • T to A, chromosome 17 at 34,568,422 bp
  • A to G, chromosome 17 at 73,913,901 bp
  • A to T, chromosome 19 at 3,896,845 bp
  • A to T, chromosome 19 at 7,604,312 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1548 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039587-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.