Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR1548Btlr/Mmmh
Stock Number:
039587-MU
Citation ID:
RRID:MMRRC_039587-MU
Other Names:
R1548 (G1), C57BL/6J-MtgxR1548Btlr
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Ints6
Name: integrator complex subunit 6
Synonyms: Notch2l, 2900075H24Rik, DICE1, Ddx26
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18130
VEGA: 14
Homologene: 8121
Ubap2
Name: ubiquitin-associated protein 2
Synonyms: 1190005K07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68926
Homologene: 73649
Foxp1
Name: forkhead box P1
Synonyms: 4932443N09Rik, 3110052D19Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 108655
HGNC: HGNC:3823
Homologene: 13092
Gpr19
Name: G protein-coupled receptor 19
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14760
HGNC: HGNC:4473
Homologene: 4476
Ddx10
Name: DEAD box helicase 10
Synonyms: 4632415A01Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 77591
VEGA: 9
HGNC: HGNC:2735
Homologene: 20922
Gpr21
Name: G protein-coupled receptor 21
Synonyms: C230004C13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 338346
HGNC: HGNC:4476
Homologene: 74546
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 11,518,594 bp
  • G to T, chromosome 1 at 74,570,104 bp
  • G to T, chromosome 1 at 130,698,083 bp
  • T to C, chromosome 1 at 136,110,937 bp
  • A to C, chromosome 2 at 29,065,138 bp
  • C to T, chromosome 2 at 37,518,072 bp
  • T to A, chromosome 2 at 38,568,895 bp
  • A to G, chromosome 2 at 53,105,537 bp
  • C to T, chromosome 2 at 116,058,702 bp
  • G to T, chromosome 2 at 164,037,171 bp
  • C to T, chromosome 2 at 164,501,519 bp
  • G to A, chromosome 2 at 167,034,464 bp
  • C to T, chromosome 4 at 41,199,872 bp
  • A to T, chromosome 4 at 56,906,233 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • A to G, chromosome 4 at 84,275,957 bp
  • T to C, chromosome 4 at 111,779,006 bp
  • T to A, chromosome 4 at 126,370,395 bp
  • C to T, chromosome 4 at 155,434,045 bp
  • A to T, chromosome 5 at 63,800,182 bp
  • G to T, chromosome 5 at 121,626,040 bp
  • G to C, chromosome 5 at 121,626,041 bp
  • G to A, chromosome 6 at 30,642,335 bp
  • T to C, chromosome 6 at 40,750,990 bp
  • G to T, chromosome 6 at 41,396,011 bp
  • A to T, chromosome 6 at 98,945,420 bp
  • G to C, chromosome 6 at 130,333,144 bp
  • G to A, chromosome 6 at 134,459,429 bp
  • A to G, chromosome 6 at 134,870,084 bp
  • T to C, chromosome 7 at 17,044,403 bp
  • C to G, chromosome 7 at 44,992,818 bp
  • A to T, chromosome 7 at 80,228,414 bp
  • T to C, chromosome 7 at 107,590,375 bp
  • G to T, chromosome 7 at 141,638,772 bp
  • A to G, chromosome 7 at 141,652,103 bp
  • A to G, chromosome 8 at 105,304,688 bp
  • G to T, chromosome 9 at 21,980,706 bp
  • T to C, chromosome 9 at 41,581,376 bp
  • T to C, chromosome 9 at 53,149,561 bp
  • C to T, chromosome 9 at 55,816,670 bp
  • T to C, chromosome 9 at 65,135,227 bp
  • A to T, chromosome 9 at 75,171,746 bp
  • C to A, chromosome 9 at 107,551,846 bp
  • C to T, chromosome 9 at 109,100,900 bp
  • T to C, chromosome 10 at 90,049,985 bp
  • C to T, chromosome 10 at 123,036,007 bp
  • A to G, chromosome 11 at 49,095,871 bp
  • A to G, chromosome 11 at 60,488,238 bp
  • C to T, chromosome 11 at 85,339,605 bp
  • T to C, chromosome 11 at 89,526,541 bp
  • A to G, chromosome 11 at 95,046,919 bp
  • C to T, chromosome 11 at 96,318,899 bp
  • G to A, chromosome 11 at 119,442,707 bp
  • T to C, chromosome 12 at 91,534,031 bp
  • A to T, chromosome 13 at 11,554,549 bp
  • T to C, chromosome 13 at 112,599,997 bp
  • C to A, chromosome 14 at 51,195,533 bp
  • G to A, chromosome 14 at 62,713,692 bp
  • C to T, chromosome 15 at 37,336,323 bp
  • A to G, chromosome 15 at 39,872,506 bp
  • A to G, chromosome 15 at 47,981,975 bp
  • A to G, chromosome 16 at 43,821,341 bp
  • A to T, chromosome 16 at 58,615,839 bp
  • A to G, chromosome 16 at 87,485,584 bp
  • G to A, chromosome 16 at 89,015,389 bp
  • T to A, chromosome 17 at 34,568,422 bp
  • A to G, chromosome 17 at 73,913,901 bp
  • A to T, chromosome 19 at 3,896,845 bp
  • A to T, chromosome 19 at 7,604,312 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1548 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039587-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.