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Strain Name:
C57BL/6J-MtgxR1977Btlr/Mmmh
Stock Number:
039990-MU
Citation ID:
RRID:MMRRC_039990-MU
Other Names:
R1977 (G1), C57BL/6J-MtgxR1977Btlr
Major Collection:

Strain Information

Nfatc2
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
Synonyms: NFAT1, NFATp, NFAT1-D
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18019
HGNC: HGNC:7776
Homologene: 7861
Pdgfc
Name: platelet-derived growth factor, C polypeptide
Synonyms: 1110064L01Rik, PDGF-C
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 54635
HGNC: HGNC:8801
Homologene: 9423
Taok3
Name: TAO kinase 3
Synonyms: A430105I05Rik, 2900006A08Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 330177
Homologene: 83279
Wdr59
Name: WD repeat domain 59
Synonyms: 5430401O09Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 319481
Homologene: 38685
Polk
Name: polymerase (DNA directed), kappa
Synonyms: Dinb1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 27015
VEGA: 13
HGNC: HGNC:9183
Homologene: 32140
Sec23ip
Name: Sec23 interacting protein
Synonyms: D7Ertd373e, p125
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 207352
Homologene: 38288
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 60,146,136 bp
  • A to G, chromosome 2 at 74,744,276 bp
  • A to G, chromosome 2 at 87,491,121 bp
  • A to T, chromosome 2 at 110,011,928 bp
  • A to T, chromosome 2 at 118,461,757 bp
  • G to A, chromosome 2 at 120,374,504 bp
  • A to G, chromosome 2 at 163,004,160 bp
  • G to A, chromosome 2 at 168,504,459 bp
  • T to A, chromosome 3 at 14,587,356 bp
  • C to T, chromosome 3 at 64,504,221 bp
  • C to T, chromosome 3 at 81,209,245 bp
  • C to T, chromosome 3 at 87,851,816 bp
  • T to A, chromosome 3 at 93,727,272 bp
  • A to G, chromosome 3 at 102,947,817 bp
  • G to T, chromosome 3 at 126,795,353 bp
  • C to A, chromosome 4 at 48,056,539 bp
  • G to A, chromosome 4 at 48,181,553 bp
  • A to G, chromosome 4 at 156,354,591 bp
  • A to G, chromosome 5 at 37,276,283 bp
  • A to C, chromosome 5 at 45,775,420 bp
  • C to A, chromosome 5 at 67,805,711 bp
  • T to C, chromosome 5 at 77,117,616 bp
  • A to T, chromosome 5 at 107,799,139 bp
  • A to T, chromosome 5 at 117,265,924 bp
  • A to G, chromosome 5 at 148,509,258 bp
  • C to T, chromosome 6 at 29,467,978 bp
  • G to T, chromosome 6 at 38,329,237 bp
  • A to G, chromosome 6 at 38,948,641 bp
  • G to A, chromosome 6 at 40,664,880 bp
  • T to A, chromosome 6 at 43,201,980 bp
  • A to T, chromosome 7 at 14,664,398 bp
  • A to G, chromosome 7 at 26,835,922 bp
  • A to T, chromosome 7 at 45,336,214 bp
  • T to A, chromosome 7 at 83,757,352 bp
  • T to C, chromosome 7 at 110,803,162 bp
  • T to A, chromosome 7 at 128,766,273 bp
  • T to C, chromosome 7 at 141,448,781 bp
  • A to T, chromosome 8 at 15,085,263 bp
  • T to A, chromosome 8 at 27,115,761 bp
  • T to A, chromosome 8 at 27,275,101 bp
  • A to G, chromosome 8 at 41,343,517 bp
  • G to A, chromosome 8 at 72,109,854 bp
  • A to T, chromosome 8 at 111,458,638 bp
  • G to T, chromosome 8 at 122,781,533 bp
  • T to A, chromosome 9 at 15,734,210 bp
  • A to T, chromosome 9 at 52,678,192 bp
  • T to A, chromosome 9 at 109,652,856 bp
  • G to A, chromosome 9 at 109,835,341 bp
  • C to T, chromosome 9 at 122,747,362 bp
  • A to T, chromosome 10 at 26,990,800 bp
  • A to T, chromosome 10 at 80,020,818 bp
  • A to G, chromosome 10 at 80,703,121 bp
  • A to G, chromosome 10 at 115,844,130 bp
  • T to C, chromosome 10 at 128,320,413 bp
  • A to G, chromosome 11 at 29,141,256 bp
  • A to C, chromosome 11 at 32,422,138 bp
  • G to T, chromosome 11 at 97,451,447 bp
  • T to C, chromosome 11 at 118,112,591 bp
  • A to G, chromosome 11 at 120,785,826 bp
  • T to A, chromosome 12 at 8,961,110 bp
  • G to C, chromosome 13 at 4,574,212 bp
  • T to C, chromosome 13 at 96,489,228 bp
  • T to A, chromosome 13 at 116,910,913 bp
  • T to A, chromosome 14 at 55,234,655 bp
  • T to C, chromosome 14 at 119,138,476 bp
  • G to A, chromosome 16 at 19,514,836 bp
  • T to A, chromosome 17 at 35,850,930 bp
  • T to C, chromosome 17 at 56,615,068 bp
  • A to G, chromosome 17 at 79,852,590 bp
  • T to C, chromosome 19 at 39,778,041 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1977 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039990-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.