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Strain Name:
C57BL/6J-MtgxR2135Btlr/Mmmh
Stock Number:
040138-MU
Citation ID:
RRID:MMRRC_040138-MU
Other Names:
R2135 (G1), C57BL/6J-MtgxR2135Btlr
Major Collection:

Strain Information

Ext1
Name: exostosin glycosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14042
HGNC: HGNC:3512
Homologene: 30957
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Timmdc1
Name: translocase of inner mitochondrial membrane domain containing 1
Synonyms: 2810021C21Rik, 4930455C21Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 76916
HGNC: HGNC:1321
Homologene: 9578
Msra
Name: methionine sulfoxide reductase A
Synonyms: MSR-A, 2310045J23Rik, 6530413P12Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 110265
HGNC: HGNC:7377
Homologene: 5812
L3mbtl2
Name: L3MBTL2 polycomb repressive complex 1 subunit
Synonyms: 4732493N06Rik, m4mbt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 214669
Homologene: 12882
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Ltn1
Name: listerin E3 ubiquitin protein ligase 1
Synonyms: 4930528H02Rik, Zfp294, Listerin, Rnf160
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78913
VEGA: 16
Homologene: 32272
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 9,747,810 bp
  • A to C, chromosome 1 at 65,161,919 bp
  • A to G, chromosome 1 at 97,104,817 bp
  • A to G, chromosome 1 at 140,428,813 bp
  • T to C, chromosome 2 at 31,640,313 bp
  • A to T, chromosome 3 at 38,980,733 bp
  • A to G, chromosome 3 at 102,778,148 bp
  • A to G, chromosome 4 at 118,142,459 bp
  • A to G, chromosome 4 at 127,951,640 bp
  • C to G, chromosome 4 at 155,429,940 bp
  • T to A, chromosome 4 at 155,429,951 bp
  • A to G, chromosome 5 at 4,064,509 bp
  • A to G, chromosome 5 at 8,427,489 bp
  • G to T, chromosome 5 at 24,526,222 bp
  • A to T, chromosome 5 at 109,737,643 bp
  • A to G, chromosome 6 at 28,431,584 bp
  • T to A, chromosome 6 at 92,189,873 bp
  • C to T, chromosome 6 at 120,720,962 bp
  • A to G, chromosome 6 at 129,622,526 bp
  • T to C, chromosome 6 at 133,105,526 bp
  • T to A, chromosome 6 at 136,723,728 bp
  • T to A, chromosome 6 at 140,580,499 bp
  • C to A, chromosome 7 at 4,340,757 bp
  • A to G, chromosome 7 at 34,371,184 bp
  • A to G, chromosome 7 at 86,231,011 bp
  • C to T, chromosome 7 at 144,411,234 bp
  • G to A, chromosome 8 at 66,512,347 bp
  • T to C, chromosome 8 at 88,591,650 bp
  • C to A, chromosome 8 at 93,499,741 bp
  • A to T, chromosome 8 at 121,883,705 bp
  • T to A, chromosome 9 at 20,300,297 bp
  • T to C, chromosome 9 at 22,468,129 bp
  • A to C, chromosome 9 at 35,839,183 bp
  • A to C, chromosome 9 at 42,340,285 bp
  • A to C, chromosome 9 at 53,450,503 bp
  • A to C, chromosome 9 at 101,211,558 bp
  • C to A, chromosome 9 at 107,526,513 bp
  • A to C, chromosome 11 at 58,529,785 bp
  • A to T, chromosome 11 at 77,055,008 bp
  • T to C, chromosome 11 at 84,153,812 bp
  • A to G, chromosome 11 at 115,794,749 bp
  • A to G, chromosome 12 at 40,745,668 bp
  • A to G, chromosome 12 at 70,894,997 bp
  • A to G, chromosome 12 at 73,545,333 bp
  • A to G, chromosome 12 at 75,952,786 bp
  • T to C, chromosome 12 at 79,246,052 bp
  • A to T, chromosome 12 at 103,202,690 bp
  • T to A, chromosome 13 at 54,720,947 bp
  • C to A, chromosome 13 at 70,801,007 bp
  • A to G, chromosome 13 at 81,524,557 bp
  • G to A, chromosome 13 at 91,972,255 bp
  • T to G, chromosome 13 at 114,826,296 bp
  • A to G, chromosome 14 at 27,448,140 bp
  • G to A, chromosome 14 at 64,123,208 bp
  • A to T, chromosome 14 at 103,145,942 bp
  • T to C, chromosome 14 at 103,208,893 bp
  • A to T, chromosome 15 at 31,509,764 bp
  • T to C, chromosome 15 at 35,937,283 bp
  • G to T, chromosome 15 at 53,101,744 bp
  • A to G, chromosome 15 at 66,782,714 bp
  • A to G, chromosome 15 at 81,682,014 bp
  • T to C, chromosome 15 at 85,816,506 bp
  • T to C, chromosome 15 at 93,397,186 bp
  • A to T, chromosome 16 at 38,498,951 bp
  • A to G, chromosome 16 at 87,382,713 bp
  • A to T, chromosome 16 at 97,743,733 bp
  • C to T, chromosome 17 at 34,857,278 bp
  • A to G, chromosome 17 at 42,317,074 bp
  • T to C, chromosome 18 at 37,974,456 bp
  • T to A, chromosome 18 at 46,990,537 bp
  • A to G, chromosome 19 at 6,112,798 bp
  • A to G, chromosome 19 at 8,708,244 bp
  • T to C, chromosome 19 at 29,646,065 bp
  • T to C, chromosome 19 at 42,044,059 bp
  • T to C, chromosome 19 at 44,534,696 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2135 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040138-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.