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Strain Name:
C57BL/6J-MtgxR2568Btlr/Mmmh
Stock Number:
040427-MU
Citation ID:
RRID:MMRRC_040427-MU
Other Names:
R2568 (G1), C57BL/6J-MtgxR2568Btlr
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Tln2
Name: talin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70549
VEGA: 9
Homologene: 56692
Agt
Name: angiotensinogen
Synonyms: Aogen, Serpina8, angiotensin precursor
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11606
HGNC: HGNC:333
Homologene: 14
Tiparp
Name: TCDD-inducible poly(ADP-ribose) polymerase
Synonyms: DDF1, PARP-7, PARP7
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99929
Homologene: 9167
Foxj2
Name: forkhead box J2
Synonyms: Fhx
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 60611
Homologene: 10187
Prdx1
Name: peroxiredoxin 1
Synonyms: OSF-3, MSP23, Tdpx2, macrophage 23kDa stress protein, macrophase stress protein 22kDa, osteoblast specific factor 3, Trx dependent peroxide reductase 2, thioredoxin dependent peroxide reductase 2, TDX2, Paga, PrxI, PAG, Prx I, prx1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18477
HGNC: HGNC:9352
Homologene: 99789
Ube3c
Name: ubiquitin protein ligase E3C
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100763
Homologene: 8783
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • CCTGCTGCTGCTGCTGCTGCTGCTGC to CCTGCTGCTGCTGCTGCTGCTGC, chromosome 1 at 64,937,781 bp
  • T to C, chromosome 1 at 120,494,785 bp
  • A to C, chromosome 1 at 134,835,771 bp
  • A to T, chromosome 1 at 135,363,964 bp
  • T to A, chromosome 1 at 156,392,749 bp
  • T to A, chromosome 1 at 162,836,259 bp
  • T to C, chromosome 2 at 36,479,974 bp
  • C to T, chromosome 2 at 66,273,469 bp
  • A to G, chromosome 2 at 82,990,431 bp
  • T to C, chromosome 2 at 92,458,880 bp
  • G to A, chromosome 2 at 112,675,874 bp
  • A to T, chromosome 2 at 129,367,322 bp
  • T to C, chromosome 2 at 129,615,648 bp
  • C to G, chromosome 2 at 155,942,090 bp
  • A to T, chromosome 3 at 29,582,931 bp
  • T to A, chromosome 3 at 65,553,130 bp
  • T to A, chromosome 3 at 108,978,848 bp
  • T to C, chromosome 3 at 124,575,365 bp
  • T to A, chromosome 4 at 34,711,566 bp
  • T to G, chromosome 4 at 116,693,800 bp
  • A to T, chromosome 4 at 120,268,764 bp
  • ACTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCT to ACTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCT, chromosome 5 at 29,637,586 bp
  • T to A, chromosome 5 at 31,665,752 bp
  • C to A, chromosome 5 at 120,710,184 bp
  • A to G, chromosome 5 at 125,848,011 bp
  • G to A, chromosome 5 at 144,843,369 bp
  • T to G, chromosome 6 at 58,935,609 bp
  • T to A, chromosome 6 at 66,776,971 bp
  • C to T, chromosome 6 at 122,828,372 bp
  • T to A, chromosome 6 at 123,742,188 bp
  • C to T, chromosome 6 at 128,327,638 bp
  • T to A, chromosome 7 at 34,216,016 bp
  • A to G, chromosome 7 at 49,597,564 bp
  • A to G, chromosome 7 at 105,056,671 bp
  • A to T, chromosome 8 at 45,795,370 bp
  • A to T, chromosome 8 at 94,934,021 bp
  • G to A, chromosome 8 at 122,862,024 bp
  • A to C, chromosome 8 at 124,556,955 bp
  • A to T, chromosome 9 at 22,279,238 bp
  • A to T, chromosome 9 at 67,229,320 bp
  • A to G, chromosome 9 at 75,123,040 bp
  • T to C, chromosome 9 at 75,151,897 bp
  • A to T, chromosome 9 at 106,063,076 bp
  • A to G, chromosome 9 at 110,097,195 bp
  • A to G, chromosome 9 at 113,878,764 bp
  • A to T, chromosome 11 at 9,333,310 bp
  • A to G, chromosome 11 at 45,951,565 bp
  • T to G, chromosome 11 at 60,708,812 bp
  • C to T, chromosome 11 at 66,080,485 bp
  • T to G, chromosome 11 at 108,404,871 bp
  • A to G, chromosome 11 at 117,772,820 bp
  • A to G, chromosome 12 at 5,091,350 bp
  • A to G, chromosome 12 at 52,887,278 bp
  • G to T, chromosome 13 at 6,575,092 bp
  • C to T, chromosome 13 at 24,883,891 bp
  • T to C, chromosome 13 at 32,722,018 bp
  • T to A, chromosome 13 at 49,530,129 bp
  • A to G, chromosome 14 at 14,999,112 bp
  • A to G, chromosome 14 at 30,082,511 bp
  • T to C, chromosome 15 at 8,201,269 bp
  • T to A, chromosome 15 at 39,084,488 bp
  • CCTTTGCGCTT to CCTT, chromosome 15 at 47,741,236 bp
  • G to T, chromosome 15 at 101,487,827 bp
  • A to G, chromosome 16 at 58,925,923 bp
  • A to G, chromosome 16 at 92,321,319 bp
  • T to A, chromosome 17 at 36,269,164 bp
  • A to G, chromosome 17 at 43,437,671 bp
  • C to T, chromosome 17 at 84,726,649 bp
  • A to G, chromosome 18 at 44,337,216 bp
  • T to C, chromosome 18 at 89,036,980 bp
  • C to A, chromosome 19 at 10,248,152 bp
  • C to G, chromosome 19 at 55,180,999 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2568 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040427-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.