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Strain Name:
C57BL/6J-MtgxR2850Btlr/Mmmh
Stock Number:
040443-MU
Citation ID:
RRID:MMRRC_040443-MU
Other Names:
R2850 (G1), C57BL/6J-MtgxR2850Btlr
Major Collection:

Strain Information

Nfat5
Name: nuclear factor of activated T cells 5
Synonyms: nfatz, TonEBP, B130038B15Rik, OREBP
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54446
HGNC: HGNC:7774
Homologene: 4811
Gnb5
Name: guanine nucleotide binding protein (G protein), beta 5
Synonyms: G beta 5, Gbeta5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14697
VEGA: 9
HGNC: HGNC:4401
Homologene: 40714
Nedd4
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: Nedd4-1, Nedd4, Nedd4a, E430025J12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17999
HGNC: HGNC:7727
Homologene: 134533
Th
Name: tyrosine hydroxylase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21823
Homologene: 307
Prkd3
Name: protein kinase D3
Synonyms: 4930557O20Rik, 5730497N19Rik, PKD3, Prkcn
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 75292
HGNC: HGNC:9408
Homologene: 2055
Itch
Name: itchy, E3 ubiquitin protein ligase
Synonyms: 6720481N21Rik, C230047C07Rik, 8030492O04Rik, AIP4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16396
Homologene: 88442
Rbpms
Name: RNA binding protein gene with multiple splicing
Synonyms: hermes, 2700019M19Rik, 2010300K22Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19663
Homologene: 38238
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 20,509,076 bp
  • A to G, chromosome 1 at 58,412,279 bp
  • A to G, chromosome 1 at 153,406,590 bp
  • T to C, chromosome 1 at 160,139,287 bp
  • G to A, chromosome 2 at 13,322,953 bp
  • C to T, chromosome 2 at 24,761,788 bp
  • A to T, chromosome 2 at 37,377,415 bp
  • T to C, chromosome 2 at 130,565,047 bp
  • C to T, chromosome 2 at 143,949,349 bp
  • A to C, chromosome 2 at 155,202,221 bp
  • T to C, chromosome 3 at 16,203,818 bp
  • A to T, chromosome 3 at 92,123,169 bp
  • A to T, chromosome 3 at 136,284,266 bp
  • T to A, chromosome 4 at 14,593,806 bp
  • T to C, chromosome 4 at 19,415,690 bp
  • T to C, chromosome 4 at 22,403,624 bp
  • T to A, chromosome 4 at 33,049,696 bp
  • A to G, chromosome 4 at 116,270,784 bp
  • T to C, chromosome 4 at 126,443,075 bp
  • T to A, chromosome 5 at 32,293,224 bp
  • A to G, chromosome 5 at 72,304,955 bp
  • G to A, chromosome 5 at 76,919,375 bp
  • T to C, chromosome 6 at 85,621,299 bp
  • C to A, chromosome 6 at 85,667,963 bp
  • C to T, chromosome 7 at 111,053,446 bp
  • T to C, chromosome 7 at 120,138,306 bp
  • G to A, chromosome 7 at 123,179,800 bp
  • T to C, chromosome 7 at 127,915,366 bp
  • G to A, chromosome 7 at 142,894,075 bp
  • A to G, chromosome 8 at 13,295,024 bp
  • G to T, chromosome 8 at 33,834,377 bp
  • G to T, chromosome 8 at 68,899,512 bp
  • T to C, chromosome 8 at 91,289,611 bp
  • A to G, chromosome 8 at 107,293,860 bp
  • T to A, chromosome 8 at 109,623,990 bp
  • T to C, chromosome 9 at 55,223,415 bp
  • A to G, chromosome 9 at 72,312,089 bp
  • C to T, chromosome 9 at 72,725,074 bp
  • A to T, chromosome 9 at 75,327,229 bp
  • T to C, chromosome 9 at 107,591,709 bp
  • C to T, chromosome 10 at 21,000,593 bp
  • T to C, chromosome 10 at 40,254,258 bp
  • G to A, chromosome 11 at 32,287,191 bp
  • G to A, chromosome 11 at 50,815,331 bp
  • A to T, chromosome 11 at 54,502,677 bp
  • T to A, chromosome 11 at 55,011,026 bp
  • G to A, chromosome 11 at 60,438,574 bp
  • A to G, chromosome 11 at 67,256,509 bp
  • A to T, chromosome 11 at 75,451,172 bp
  • T to A, chromosome 13 at 12,275,179 bp
  • T to C, chromosome 15 at 103,752,082 bp
  • A to G, chromosome 16 at 33,269,642 bp
  • A to G, chromosome 16 at 90,774,256 bp
  • A to G, chromosome 17 at 35,124,581 bp
  • C to A, chromosome 17 at 48,249,641 bp
  • A to G, chromosome 17 at 78,954,596 bp
  • T to A, chromosome 18 at 70,483,451 bp
  • T to C, chromosome 19 at 13,063,570 bp
  • T to A, chromosome 19 at 18,792,090 bp
  • C to A, chromosome 19 at 37,409,493 bp
  • T to C, chromosome X at 111,593,023 bp
  • T to A, chromosome X at 153,559,278 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2850 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040443-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.