Strain Detail Sheet
- A to G, chromosome 1 at 33,533,299 bp
- AGAAAG to A, chromosome 1 at 49,310,929 bp
- A to G, chromosome 1 at 92,637,406 bp
- G to A, chromosome 2 at 31,453,157 bp
- A to G, chromosome 2 at 87,002,374 bp
- T to A, chromosome 2 at 164,027,676 bp
- A to G, chromosome 2 at 166,795,472 bp
- T to C, chromosome 3 at 36,221,923 bp
- C to A, chromosome 3 at 93,014,707 bp
- G to A, chromosome 3 at 138,555,437 bp
- T to C, chromosome 4 at 150,239,568 bp
- T to A, chromosome 5 at 30,256,088 bp
- A to G, chromosome 5 at 130,019,869 bp
- GCG to GCGACGGCGACG, chromosome 7 at 97,579,907 bp
- T to C, chromosome 7 at 101,859,246 bp
- A to T, chromosome 7 at 127,324,537 bp
- C to T, chromosome 9 at 59,948,433 bp
- C to T, chromosome 10 at 12,698,042 bp
- T to A, chromosome 11 at 23,343,640 bp
- T to G, chromosome 11 at 53,531,981 bp
- T to A, chromosome 11 at 58,566,826 bp
- T to C, chromosome 11 at 73,711,124 bp
- C to T, chromosome 11 at 84,040,899 bp
- T to C, chromosome 11 at 120,642,135 bp
- G to T, chromosome 12 at 69,756,599 bp
- T to C, chromosome 12 at 111,275,680 bp
- T to A, chromosome 13 at 67,347,938 bp
- G to A, chromosome 13 at 81,578,772 bp
- A to T, chromosome 14 at 96,346,880 bp
- A to G, chromosome 18 at 64,341,520 bp
- A to G, chromosome 19 at 11,618,948 bp
- T to C, chromosome 19 at 47,797,116 bp
- T to C, chromosome 19 at 52,991,697 bp
- Genotyping Protocol(s)
- Center protocol and contact for technical support will be shipped with mice.
Colony Surveillance Program and Current Health Reports
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
| MMRRC Item # | Description | Distribution Fee / Unit (US $) *Shipping & Handling not included* |
Units | Notes |
|---|---|---|---|---|
| 040916-MU-RESUS | Litter recovered from cryo-archive | $5,475.00 / Non-Profit | Litter | Recovered litter4; additional fees for any special requests. |
| Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information. | ||||
Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.