Strain Name:
C57BL/6J-MtgxR4354Btlr/Mmmh
Stock Number:
041107-MU
Citation ID:
RRID:MMRRC_041107-MU
Other Names:
R4354 (G1), C57BL/6J-MtgxR4354Btlr
Major Collection:

Gene Information

Chgb
Name: chromogranin B
Synonyms: Scg-1, secretogranin I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12653
HGNC: HGNC:1930
Homologene: 1375
Gpa33
Name: glycoprotein A33 (transmembrane)
Synonyms: A33 antigen, 2210401D16Rik, 2010310L10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 59290
HGNC: HGNC:4445
Homologene: 4245
Mon2
Name: MON2 homolog, regulator of endosome to Golgi trafficking
Synonyms: SF21, 2610528O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67074
VEGA: 10
Homologene: 44309
Adamtsl1
Name: ADAMTS-like 1
Synonyms: punctin-1, 6720426B09Rik, 5930437A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 77739
Homologene: 64642
Irf7
Name: interferon regulatory factor 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54123
HGNC: HGNC:6122
Homologene: 128624
Hspg2
Name: perlecan (heparan sulfate proteoglycan 2)
Synonyms: Plc, per, perlecan, Pcn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 15530
HGNC: HGNC:5273
Homologene: 68473
Riok2
Name: RIO kinase 2
Synonyms: 2010110K24Rik, 2410085M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 67045
VEGA: 17
Homologene: 6760
Pold3
Name: polymerase (DNA-directed), delta 3, accessory subunit
Synonyms: P68, 2410142G14Rik, P66, GC12, C85233
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67967
Homologene: 38202
Nudt9
Name: nudix (nucleoside diphosphate linked moiety X)-type motif 9
Synonyms: 1190002C07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74167
HGNC: HGNC:8056
Homologene: 11435
AU040320
Name: expressed sequence AU040320
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100317
Homologene: 11764
Alg8
Name: asparagine-linked glycosylation 8 (alpha-1,3-glucosyltransferase)
Synonyms: LOC381903
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381903
Homologene: 6931
Nle1
Name: notchless homolog 1
Synonyms: l11Jus1, Nle, notchless, l11Jus4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217011
Homologene: 5494
Tlr8
Name: toll-like receptor 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 170744
Homologene: 44054
Susd2
Name: sushi domain containing 2
Synonyms: 1200011D11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 71733
VEGA: 10
Homologene: 10481
Kcnv1
Name: potassium channel, subfamily V, member 1
Synonyms: 2700023A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 67498
VEGA: 15
Homologene: 22811
Fhdc1
Name: FH2 domain containing 1
Synonyms: 6330505N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229474
Homologene: 18920
Abcb4
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 4
Synonyms: Pgy2, Mdr2, mdr-2, Pgy-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18670
HGNC: HGNC:45
Homologene: 136368
Loxhd1
Name: lipoxygenase homology domains 1
Synonyms: sba, 1700096C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240411
Neu4
Name: sialidase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241159
Homologene: 15433
Pdzd8
Name: PDZ domain containing 8
Synonyms: Pdzk8, A630041P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107368
VEGA: 19
Homologene: 14879
Abca8b
Name: ATP-binding cassette, sub-family A (ABC1), member 8b
Synonyms: Abca8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 27404
HGNC: HGNC:38
Homologene: 56029
Glis3
Name: GLIS family zinc finger 3
Synonyms: E330013K21Rik, 4833409N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226075
Homologene: 34989
Ablim1
Name: actin-binding LIM protein 1
Synonyms: 4833406P10Rik, Limab1, 2210411C18Rik, abLIM-L, abLIM-M, 2610209L21Rik, 9330196J19Rik, abLIM-S
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226251
HGNC: HGNC:78
Homologene: 40994
Nprl3
Name: nitrogen permease regulator-like 3
Synonyms: HS-26, Phg, -14 gene, HS-40, Prox1, Mare, m(alpha)RE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17168
Homologene: 8091
Tbc1d7
Name: TBC1 domain family, member 7
Synonyms: 2610009C09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 67046
VEGA: 13
Homologene: 9538
Pga5
Name: pepsinogen 5, group I
Synonyms: 1110035E17Rik, Pepf, pepsinogen A5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 58803
VEGA: 19
Homologene: 105932
Ube2j1
Name: ubiquitin-conjugating enzyme E2J 1
Synonyms: 1110030I22Rik, Ubc6p, Ncube, 0710008M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56228
Homologene: 41090
4930578I06Rik
Name: RIKEN cDNA 4930578I06 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67750
VEGA: 14
Homologene: 49859
Abi3bp
Name: ABI gene family, member 3 (NESH) binding protein
Synonyms: TARSH, eratin, 5033411B22Rik, D930038M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 320712
VEGA: 16
Homologene: 134172
Nab1
Name: Ngfi-A binding protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17936
HGNC: HGNC:7626
Homologene: 4352
Alms1-ps1
Name: ALMS1, centrosome and basal body associated, pseudogene 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 100043493
Rab3gap1
Name: RAB3 GTPase activating protein subunit 1
Synonyms: 4732493F09Rik, 1700003B17Rik, p130
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226407
Homologene: 45617
Ptgir
Name: prostaglandin I receptor (IP)
Synonyms: IP, prostacyclin receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19222
HGNC: HGNC:9602
Homologene: 7496
Olfr368
Name: olfactory receptor 368
Synonyms: GA_x6K02T2NLDC-34015743-34016726, MOR178-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258371
HGNC: HGNC:8331
Homologene: 71970
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 52,490,696 bp
  • C to T, chromosome 1 at 94,024,557 bp
  • A to G, chromosome 1 at 127,915,641 bp
  • A to G, chromosome 1 at 166,163,835 bp
  • T to C, chromosome 2 at 37,331,876 bp
  • A to T, chromosome 2 at 132,793,944 bp
  • C to A, chromosome 3 at 84,444,826 bp
  • T to C, chromosome 4 at 33,049,682 bp
  • A to T, chromosome 4 at 86,156,684 bp
  • A to G, chromosome 4 at 126,854,399 bp
  • C to A, chromosome 4 at 137,468,911 bp
  • A to T, chromosome 5 at 8,918,771 bp
  • A to G, chromosome 5 at 104,058,111 bp
  • C to T, chromosome 6 at 85,755,635 bp
  • A to G, chromosome 7 at 16,906,869 bp
  • A to G, chromosome 7 at 97,388,559 bp
  • T to C, chromosome 7 at 100,100,617 bp
  • T to C, chromosome 7 at 141,265,270 bp
  • G to T, chromosome 10 at 75,639,728 bp
  • T to C, chromosome 10 at 123,026,983 bp
  • T to A, chromosome 11 at 32,234,906 bp
  • A to G, chromosome 11 at 82,906,431 bp
  • A to C, chromosome 11 at 109,971,692 bp
  • T to C, chromosome 13 at 43,169,868 bp
  • C to T, chromosome 14 at 63,973,209 bp
  • G to A, chromosome 15 at 45,114,444 bp
  • T to C, chromosome 16 at 56,532,951 bp
  • T to A, chromosome 17 at 17,390,001 bp
  • T to C, chromosome 18 at 77,395,427 bp
  • C to T, chromosome 19 at 10,674,826 bp
  • C to A, chromosome 19 at 28,317,270 bp
  • C to T, chromosome 19 at 57,155,278 bp
  • T to C, chromosome 19 at 59,345,481 bp
  • T to G, chromosome X at 167,242,872 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4354 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041107-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.