Strain Name:
C57BL/6J-MtgxR4454Btlr/Mmmh
Stock Number:
041714-MU
Citation ID:
RRID:MMRRC_041714-MU
Other Names:
R4454 (G1), C57BL/6J-MtgxR4454Btlr
Major Collection:

Gene Information

Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398
Ap4e1
Name: adaptor-related protein complex AP-4, epsilon 1
Synonyms: 2310033A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 108011
HGNC: HGNC:573
Homologene: 22397
Cdkn2d
Name: cyclin dependent kinase inhibitor 2D
Synonyms: p19, INK4d, p19INK4d, INK4d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12581
VEGA: 9
HGNC: HGNC:1790
Homologene: 36081
Eya1
Name: EYA transcriptional coactivator and phosphatase 1
Synonyms: bor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14048
HGNC: HGNC:3519
Homologene: 74943
Resp18
Name: regulated endocrine-specific protein 18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19711
Homologene: 7516
Slc25a13
Name: solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 13
Synonyms: Ctrn, citrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 50799
Homologene: 22800
Snapc3
Name: small nuclear RNA activating complex, polypeptide 3
Synonyms: 5031401C21Rik, 4930558A07Rik, 1810020H02Rik, E030018J20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 77634
Homologene: 31130
Atf6
Name: activating transcription factor 6
Synonyms: ESTM49, 9130025P16Rik, Atf6alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226641
HGNC: HGNC:791
Homologene: 32015
Med4
Name: mediator complex subunit 4
Synonyms: HSPC126, p36 TRAP/SMCC/PC2 subunit, DRIP36, 2410046H15Rik, MED4, TRAP36, Vdrip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67381
VEGA: 14
Homologene: 8568
Dmxl1
Name: Dmx-like 1
Synonyms: C630007L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240283
HGNC: HGNC:2937
Homologene: 21136
Sap130
Name: Sin3A associated protein
Synonyms: 2610304F09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 269003
VEGA: 18
Homologene: 11577
Nav2
Name: neuron navigator 2
Synonyms: POMFIL2, Unc53H2, HELAD1, RAINB2, 5330421F07Rik, Rainb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78286
Homologene: 52330
Topbp1
Name: topoisomerase (DNA) II binding protein 1
Synonyms: D430026L04Rik, 2810429C13Rik, 1110031N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235559
Homologene: 38262
Robo2
Name: roundabout guidance receptor 2
Synonyms: 9430089E08Rik, D230004I22Rik, 2600013A04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 268902
Homologene: 43188
Rad51
Name: RAD51 recombinase
Synonyms: Reca, Rad51a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19361
HGNC: HGNC:9817
Homologene: 2155
Cldn6
Name: claudin 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 54419
HGNC: HGNC:2048
Homologene: 10300
Cthrc1
Name: collagen triple helix repeat containing 1
Synonyms: 1110014B07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 68588
Homologene: 16320
Zfy1
Name: zinc finger protein 1, Y-linked
Synonyms: Zfy-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
NCBI: 22767
Homologene: 56456
Thsd1
Name: thrombospondin, type I, domain 1
Synonyms: 4833423O18Rik, Tmtsp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 56229
Homologene: 10264
Atp4a
Name: ATPase, H+/K+ exchanging, gastric, alpha polypeptide
Synonyms: H+K+-transporting alpha 1, H+/K+-ATPase alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11944
HGNC: HGNC:819
Homologene: 68081
Baiap3
Name: BAI1-associated protein 3
Synonyms: LOC381076
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 545192
HGNC: HGNC:948
Homologene: 20844
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 94109
Homologene: 69536
Mslnl
Name: mesothelin-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 328783
VEGA: 17
Homologene: 18633
Epha5
Name: Eph receptor A5
Synonyms: Cek7, bsk, Els1, Rek7, Hek7, Ehk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13839
HGNC: HGNC:3389
Homologene: 55824
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Liph
Name: lipase, member H
Synonyms: PLA1B, mPA-PLA1, C130037N08Rik, Lpdlr, D16Wsu119e, LPDLR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 239759
VEGA: 16
Homologene: 71802
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241516
Homologene: 110349
Dnah9
Name: dynein, axonemal, heavy chain 9
Synonyms: D11Ertd686e, Dnahc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237806
HGNC: HGNC:2953
Homologene: 20357
Cracdl
Name: capping protein inhibiting regulator of actin like
Synonyms: 2010300C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72097
Homologene: 19208
Fgd5
Name: FYVE, RhoGEF and PH domain containing 5
Synonyms: ZFYVE23, C330025N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232237
Homologene: 27798
Dusp26
Name: dual specificity phosphatase 26 (putative)
Synonyms: 2310043K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66959
Homologene: 11421
Egr2
Name: early growth response 2
Synonyms: Krox20, Krox-20, Egr-2, Zfp-25, NGF1-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 13654
HGNC: HGNC:3239
Homologene: 20123
Pramel25
Name: PRAME like 25
Synonyms: MGC:91194, Gm13023
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 194227
Homologene: 103830
Fam227b
Name: family with sequence similarity 227, member B
Synonyms: 4930525F21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 75823
Homologene: 27384
Shoc1
Name: shortage in chiasmata 1
Synonyms: LOC242489, Gm426, AI481877, Mzip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100155
Homologene: 79783
Olfr904
Name: olfactory receptor 904
Synonyms: GA_x6K02T2PVTD-32156773-32157705, MOR167-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258797
VEGA: 9
Homologene: 133629
Sspo
Name: SCO-spondin
Synonyms: C79529, Scospondin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243369
Homologene: 45453
Tbc1d16
Name: TBC1 domain family, member 16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 207592
Homologene: 10380
Crocc
Name: ciliary rootlet coiled-coil, rootletin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230872
Homologene: 16811
Thrb
Name: thyroid hormone receptor beta
Synonyms: T3Rbeta, T3R[b], Nr1a2, TR beta, c-erbAbeta, Thrb2, Thrb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21834
Homologene: 36025
Tnfrsf13b
Name: tumor necrosis factor receptor superfamily, member 13b
Synonyms: Taci, 1200009E08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 57916
Homologene: 49320
Pgc
Name: progastricsin (pepsinogen C)
Synonyms: Upg-1, Upg1, 2210410L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 109820
HGNC: HGNC:8890
Homologene: 55669
Cpa5
Name: carboxypeptidase A5
Synonyms: 4930430M09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74649
Homologene: 62246
Sh3tc2
Name: SH3 domain and tetratricopeptide repeats 2
Synonyms: D430044G18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225608
VEGA: 18
Homologene: 11596
Zfp995
Name: zinc finger protein 995
Synonyms: 2210404O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 70081
Homologene: 133253
Mbd3
Name: methyl-CpG binding domain protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17192
HGNC: HGNC:6918
Homologene: 2917
Olfr728
Name: olfactory receptor 728
Synonyms: GA_x6K02T2PMLR-5831021-5830086, MOR246-1P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258039
Homologene: 74224
Ddo
Name: D-aspartate oxidase
Synonyms: 5730402C02Rik, 5330420D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70503
VEGA: 10
HGNC: HGNC:2727
Homologene: 101531
C2cd4d
Name: C2 calcium-dependent domain containing 4D
Synonyms: LOC271944, Gm659
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 271944
Homologene: 86310
Rpl31-ps17
Name: ribosomal protein L31, pseudogene 17
Synonyms: Gm16382
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 673582
Pcdha10
Name: protocadherin alpha 10
Synonyms: Cnr8, Cnr3, Crnr8, Crnr3, Pcdha14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12943
VEGA: 18
HGNC: HGNC:8666
Homologene: 135720
Gm12034
Name: predicted gene 12034
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 100502908
Asmt
Name: acetylserotonin O-methyltransferase
Synonyms: Hiomt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 107626
VEGA: X
HGNC: HGNC:750
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 14,183,196 bp
  • T to C, chromosome 1 at 37,624,753 bp
  • A to G, chromosome 1 at 75,274,497 bp
  • C to T, chromosome 1 at 170,794,039 bp
  • C to A, chromosome 2 at 76,785,806 bp
  • T to C, chromosome 2 at 76,946,913 bp
  • G to T, chromosome 2 at 82,990,776 bp
  • C to T, chromosome 2 at 119,131,568 bp
  • T to A, chromosome 2 at 126,146,268 bp
  • T to A, chromosome 2 at 127,047,141 bp
  • T to A, chromosome 3 at 94,363,747 bp
  • T to C, chromosome 4 at 59,092,383 bp
  • A to G, chromosome 4 at 83,418,759 bp
  • T to C, chromosome 4 at 141,020,405 bp
  • A to G, chromosome 4 at 143,792,824 bp
  • T to C, chromosome 5 at 84,156,444 bp
  • G to A, chromosome 6 at 6,095,222 bp
  • A to G, chromosome 6 at 30,626,324 bp
  • G to A, chromosome 6 at 48,487,225 bp
  • C to T, chromosome 6 at 91,989,186 bp
  • G to A, chromosome 7 at 30,720,225 bp
  • A to T, chromosome 7 at 49,548,544 bp
  • A to T, chromosome 8 at 15,945,011 bp
  • T to C, chromosome 8 at 22,243,578 bp
  • A to G, chromosome 8 at 31,094,144 bp
  • C to G, chromosome 9 at 21,290,889 bp
  • C to A, chromosome 9 at 38,464,642 bp
  • T to C, chromosome 9 at 103,344,871 bp
  • G to T, chromosome 10 at 12,727,840 bp
  • A to T, chromosome 10 at 40,647,547 bp
  • GAA to GA, chromosome 10 at 67,539,903 bp
  • A to T, chromosome 10 at 80,393,983 bp
  • T to A, chromosome 11 at 20,446,476 bp
  • T to C, chromosome 11 at 61,141,438 bp
  • T to G, chromosome 11 at 66,147,389 bp
  • G to A, chromosome 11 at 119,157,873 bp
  • C to T, chromosome 12 at 54,701,612 bp
  • T to A, chromosome 14 at 18,011,187 bp
  • T to C, chromosome 14 at 50,140,496 bp
  • A to G, chromosome 14 at 73,518,062 bp
  • C to A, chromosome 15 at 39,077,013 bp
  • T to C, chromosome 16 at 21,984,268 bp
  • A to T, chromosome 16 at 74,352,519 bp
  • G to A, chromosome 17 at 21,879,951 bp
  • T to C, chromosome 17 at 23,681,086 bp
  • T to A, chromosome 17 at 25,249,536 bp
  • G to A, chromosome 17 at 25,742,934 bp
  • A to G, chromosome 17 at 47,732,410 bp
  • C to T, chromosome 18 at 31,711,360 bp
  • G to A, chromosome 18 at 37,007,373 bp
  • A to G, chromosome 18 at 49,893,332 bp
  • A to T, chromosome 18 at 62,007,773 bp
  • A to T, chromosome X at 170,672,721 bp
  • G to T, chromosome Y at 725,518 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4454 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041714-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.