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Strain Name:
C57BL/6J-MtgxR4893Btlr/Mmmh
Stock Number:
042498-MU
Citation ID:
RRID:MMRRC_042498-MU
Other Names:
R4893 (G1), C57BL/6J-MtgxR4893Btlr
Major Collection:

Strain Information

Ppp1r1b
Name: protein phosphatase 1, regulatory inhibitor subunit 1B
Synonyms: DARPP-32, Darpp32
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19049
HGNC: HGNC:9287
Homologene: 12972
Nos1
Name: nitric oxide synthase 1, neuronal
Synonyms: nNOS, bNOS, Nos-1, NO, 2310005C01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18125
HGNC: HGNC:7872
Homologene: 37327
Prkcd
Name: protein kinase C, delta
Synonyms: PKCdelta, PKC[d], Pkcd, D14Ertd420e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18753
HGNC: HGNC:9399
Homologene: 55963
Mbtps1
Name: membrane-bound transcription factor peptidase, site 1
Synonyms: subtilisin/kexin isozyme-1, SKI-1, S1P, 0610038M03Rik, site-1 protease
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56453
Homologene: 2808
Arfgef2
Name: ARF guanine nucleotide exchange factor 2
Synonyms: BIG2, E230011G24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99371
Homologene: 111880
Pdzd2
Name: PDZ domain containing 2
Synonyms: 4930537L06Rik, LOC223364, A930022H17Rik, Pdzk3, Gm21706
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68070
Homologene: 23393
Csnk1a1
Name: casein kinase 1, alpha 1
Synonyms: 4632404G05Rik, 2610208K14Rik, 5430427P18Rik, CK1a
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93687
HGNC: HGNC:2451
Homologene: 111694
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 58,413,066 bp
  • A to G, chromosome 1 at 79,777,865 bp
  • A to G, chromosome 1 at 80,288,850 bp
  • A to T, chromosome 1 at 105,646,711 bp
  • A to T, chromosome 1 at 136,051,179 bp
  • T to A, chromosome 1 at 139,489,839 bp
  • T to A, chromosome 2 at 25,053,676 bp
  • T to A, chromosome 2 at 122,455,216 bp
  • C to A, chromosome 2 at 150,629,866 bp
  • T to C, chromosome 2 at 154,546,754 bp
  • T to C, chromosome 2 at 156,365,184 bp
  • T to G, chromosome 2 at 166,866,956 bp
  • C to T, chromosome 2 at 172,380,524 bp
  • G to A, chromosome 2 at 178,068,203 bp
  • A to T, chromosome 2 at 179,847,419 bp
  • T to G, chromosome 3 at 30,679,297 bp
  • A to T, chromosome 3 at 64,406,255 bp
  • C to T, chromosome 3 at 144,925,173 bp
  • A to T, chromosome 4 at 87,226,908 bp
  • C to T, chromosome 4 at 126,233,222 bp
  • A to G, chromosome 4 at 127,194,983 bp
  • A to G, chromosome 4 at 136,659,753 bp
  • C to T, chromosome 4 at 138,281,415 bp
  • A to T, chromosome 4 at 148,068,458 bp
  • G to A, chromosome 4 at 155,889,541 bp
  • T to G, chromosome 5 at 30,200,478 bp
  • T to C, chromosome 5 at 64,105,940 bp
  • T to A, chromosome 5 at 98,004,072 bp
  • T to C, chromosome 5 at 107,908,002 bp
  • G to T, chromosome 5 at 114,064,877 bp
  • C to A, chromosome 5 at 117,952,877 bp
  • T to C, chromosome 5 at 123,037,682 bp
  • T to C, chromosome 5 at 143,138,019 bp
  • T to A, chromosome 6 at 17,104,596 bp
  • T to C, chromosome 7 at 3,705,911 bp
  • A to T, chromosome 8 at 70,030,640 bp
  • G to T, chromosome 8 at 108,957,007 bp
  • A to T, chromosome 8 at 109,638,394 bp
  • A to G, chromosome 8 at 116,971,165 bp
  • G to A, chromosome 8 at 119,518,193 bp
  • A to G, chromosome 9 at 49,074,025 bp
  • A to G, chromosome 9 at 49,415,683 bp
  • T to C, chromosome 9 at 62,068,495 bp
  • T to A, chromosome 9 at 80,228,877 bp
  • C to G, chromosome 9 at 108,849,421 bp
  • G to C, chromosome 9 at 122,166,320 bp
  • T to C, chromosome 10 at 23,985,400 bp
  • C to T, chromosome 10 at 80,322,896 bp
  • C to A, chromosome 10 at 128,123,415 bp
  • T to C, chromosome 11 at 4,918,214 bp
  • A to T, chromosome 11 at 94,491,996 bp
  • A to G, chromosome 11 at 98,355,344 bp
  • C to T, chromosome 12 at 69,920,496 bp
  • G to T, chromosome 12 at 71,164,546 bp
  • A to T, chromosome 12 at 71,164,547 bp
  • T to C, chromosome 12 at 76,329,969 bp
  • A to T, chromosome 13 at 16,026,549 bp
  • A to G, chromosome 13 at 54,681,932 bp
  • T to C, chromosome 14 at 14,328,852 bp
  • A to G, chromosome 14 at 26,710,170 bp
  • A to T, chromosome 14 at 30,599,425 bp
  • G to A, chromosome 14 at 63,201,596 bp
  • T to G, chromosome 15 at 12,136,542 bp
  • T to C, chromosome 15 at 12,385,343 bp
  • T to C, chromosome 15 at 36,197,846 bp
  • T to A, chromosome 15 at 80,691,955 bp
  • A to G, chromosome 15 at 101,850,120 bp
  • T to C, chromosome 16 at 17,377,036 bp
  • A to G, chromosome 16 at 19,486,903 bp
  • T to C, chromosome 16 at 32,794,672 bp
  • T to A, chromosome 16 at 49,001,950 bp
  • C to A, chromosome 16 at 96,427,821 bp
  • C to T, chromosome 16 at 97,835,648 bp
  • A to T, chromosome 17 at 9,422,231 bp
  • T to C, chromosome 17 at 25,568,989 bp
  • T to C, chromosome 17 at 34,648,612 bp
  • C to A, chromosome 17 at 85,613,685 bp
  • T to C, chromosome 18 at 21,557,935 bp
  • T to C, chromosome 18 at 23,569,667 bp
  • G to T, chromosome 18 at 30,282,000 bp
  • T to C, chromosome 18 at 31,941,811 bp
  • T to C, chromosome 18 at 61,585,301 bp
  • A to G, chromosome 19 at 4,171,015 bp
  • A to G, chromosome 19 at 9,557,954 bp
  • T to A, chromosome 19 at 44,153,771 bp
  • T to A, chromosome 19 at 57,381,756 bp
  • C to A, chromosome 19 at 58,771,421 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4893 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042498-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.