Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR5053Btlr/Mmmh
Stock Number:
042643-MU
Citation ID:
RRID:MMRRC_042643-MU
Other Names:
R5053 (G1), C57BL/6J-MtgxR5053Btlr
Major Collection:

Strain Information

Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66860
Homologene: 18946
Prpf6
Name: pre-mRNA splicing factor 6
Synonyms: 1190003A07Rik, U5-102K, ANT-1, 2610031L17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68879
Homologene: 5368
Phf20
Name: PHD finger protein 20
Synonyms: 6820402O20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228829
Homologene: 9507
Adsl
Name: adenylosuccinate lyase
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 11564
HGNC: HGNC:291
Homologene: 12
Zranb2
Name: zinc finger, RAN-binding domain containing 2
Synonyms: Znf265, Zis, Zfp265
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 53861
Plxna1
Name: plexin A1
Synonyms: NOV, Plxn1, 2600013D04Rik, PlexA1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18844
HGNC: HGNC:9099
Homologene: 56426
Sf3b1
Name: splicing factor 3b, subunit 1
Synonyms: SAP155, Prp10, SF3b155, 2810001M05Rik, Targ4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 81898
Homologene: 6696
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 54,735,089 bp
  • A to T, chromosome 1 at 54,997,177 bp
  • A to G, chromosome 1 at 82,286,922 bp
  • A to G, chromosome 1 at 179,786,784 bp
  • A to T, chromosome 2 at 4,603,921 bp
  • C to T, chromosome 2 at 59,820,263 bp
  • A to G, chromosome 2 at 86,413,338 bp
  • C to A, chromosome 2 at 88,930,253 bp
  • A to G, chromosome 2 at 155,962,928 bp
  • A to C, chromosome 2 at 156,126,398 bp
  • T to C, chromosome 2 at 156,273,862 bp
  • T to C, chromosome 2 at 181,649,453 bp
  • T to A, chromosome 3 at 63,364,849 bp
  • T to C, chromosome 3 at 144,911,121 bp
  • T to A, chromosome 3 at 157,541,159 bp
  • T to A, chromosome 4 at 41,387,315 bp
  • T to C, chromosome 4 at 107,374,218 bp
  • T to A, chromosome 4 at 126,152,397 bp
  • T to G, chromosome 4 at 138,880,375 bp
  • A to G, chromosome 4 at 152,544,462 bp
  • A to T, chromosome 5 at 57,721,601 bp
  • A to T, chromosome 5 at 100,083,740 bp
  • G to T, chromosome 5 at 100,393,214 bp
  • A to G, chromosome 5 at 111,581,763 bp
  • C to A, chromosome 5 at 140,760,727 bp
  • G to T, chromosome 6 at 29,496,958 bp
  • A to G, chromosome 6 at 41,685,335 bp
  • A to T, chromosome 6 at 89,322,460 bp
  • T to A, chromosome 6 at 149,147,268 bp
  • T to C, chromosome 7 at 11,930,314 bp
  • G to A, chromosome 7 at 14,580,975 bp
  • C to T, chromosome 7 at 18,345,934 bp
  • T to A, chromosome 7 at 27,476,522 bp
  • C to T, chromosome 7 at 43,617,146 bp
  • G to A, chromosome 7 at 43,692,077 bp
  • C to A, chromosome 7 at 56,323,580 bp
  • G to T, chromosome 7 at 105,617,761 bp
  • A to G, chromosome 7 at 108,055,534 bp
  • G to T, chromosome 9 at 50,788,172 bp
  • A to T, chromosome 9 at 66,470,326 bp
  • A to T, chromosome 9 at 107,514,864 bp
  • A to G, chromosome 10 at 18,140,345 bp
  • A to T, chromosome 10 at 80,904,655 bp
  • T to A, chromosome 10 at 107,563,202 bp
  • C to T, chromosome 10 at 127,286,441 bp
  • C to A, chromosome 11 at 24,164,068 bp
  • G to A, chromosome 11 at 50,985,310 bp
  • C to T, chromosome 11 at 100,472,200 bp
  • T to A, chromosome 12 at 103,104,748 bp
  • T to C, chromosome 12 at 104,343,214 bp
  • C to T, chromosome 12 at 118,849,604 bp
  • T to C, chromosome 13 at 12,599,906 bp
  • T to C, chromosome 14 at 21,847,696 bp
  • C to A, chromosome 14 at 26,608,583 bp
  • T to A, chromosome 14 at 50,710,511 bp
  • C to A, chromosome 15 at 10,325,385 bp
  • T to C, chromosome 15 at 80,960,450 bp
  • A to T, chromosome 15 at 101,137,369 bp
  • G to A, chromosome 15 at 102,447,258 bp
  • T to C, chromosome 16 at 10,576,597 bp
  • T to A, chromosome 16 at 20,651,893 bp
  • T to C, chromosome 16 at 44,217,187 bp
  • T to A, chromosome 16 at 95,524,534 bp
  • A to G, chromosome 17 at 42,710,443 bp
  • C to T, chromosome 19 at 26,886,413 bp
  • G to A, chromosome 19 at 40,001,887 bp
  • A to G, chromosome 19 at 46,709,054 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5053 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042643-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.