Strain Name:
C57BL/6J-MtgxR5461Btlr/Mmmh
Stock Number:
043023-MU
Citation ID:
RRID:MMRRC_043023-MU
Other Names:
R5461 (G1), C57BL/6J-MtgxR5461Btlr
Major Collection:

Gene Information

Nrp2
Name: neuropilin 2
Synonyms: Npn2, NP2, NP-2, 1110048P06Rik, Npn-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18187
HGNC: HGNC:8005
Homologene: 2875
Itgb8
Name: integrin beta 8
Synonyms: 4832412O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 320910
HGNC: HGNC:6163
Homologene: 18567
Ep400
Name: E1A binding protein p400
Synonyms: p400, 1700020J09Rik, mDomino
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 75560
Homologene: 38779
Shmt1
Name: serine hydroxymethyltransferase 1 (soluble)
Synonyms: mshmt, Shmt, mshmt1, mshmt2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20425
Homologene: 3074
Mcm3
Name: minichromosome maintenance complex component 3
Synonyms: Mcmd, P1, p1.m
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17215
HGNC: HGNC:6945
Homologene: 1791
Exoc2
Name: exocyst complex component 2
Synonyms: Sec5l1, Sec5, 2410030I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66482
Homologene: 10122
Tnip1
Name: TNFAIP3 interacting protein 1
Synonyms: A20-binding inhibitor of NF-kappa B activation, Nef, VAN, ABIN1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 57783
Homologene: 31355
Bptf
Name: bromodomain PHD finger transcription factor
Synonyms: 9430093H17Rik, Falz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 207165
HGNC: HGNC:3581
Homologene: 114397
Ncor2
Name: nuclear receptor co-repressor 2
Synonyms: SMRT, SMRTe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20602
HGNC: HGNC:7673
Homologene: 31370
Usp10
Name: ubiquitin specific peptidase 10
Synonyms: 2610014N07Rik, Uchrp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 22224
Homologene: 31294
Topbp1
Name: topoisomerase (DNA) II binding protein 1
Synonyms: 1110031N14Rik, 2810429C13Rik, D430026L04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235559
Homologene: 38262
Wwc1
Name: WW, C2 and coiled-coil domain containing 1
Synonyms: Kibra
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 211652
Homologene: 69180
Rtf2
Name: replication termination factor 2
Synonyms: 1700067C04Rik, 2410001C21Rik, Rtfdc1, 5730427M17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 66404
Homologene: 9501
Pikfyve
Name: phosphoinositide kinase, FYVE type zinc finger containing
Synonyms: PipkIII, 5230400C17Rik, Pip5k3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18711
Homologene: 32115
Poc1a
Name: POC1 centriolar protein A
Synonyms: cha, Wdr51a, 2510040D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 70235
Homologene: 51460
Crim1
Name: cysteine rich transmembrane BMP regulator 1 (chordin like)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 50766
VEGA: 17
HGNC: HGNC:2359
Homologene: 9510
Rcc1
Name: regulator of chromosome condensation 1
Synonyms: 4931417M11Rik, Chc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100088
HGNC: HGNC:1913
Homologene: 55567
4930522L14Rik
Name: RIKEN cDNA 4930522L14 gene
Synonyms: Gm42152
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100041734
Zfp949
Name: zinc finger protein 949
Synonyms: Nczf, 4930422I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 71640
Homologene: 129930
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: hy-3, hy3, 4930545D19Rik, hyrh, 1700034M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244653
Homologene: 52118
Otop1
Name: otopetrin 1
Synonyms: tlt, A530025J20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 21906
Homologene: 35244
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: D330014H01Rik, Dnahc2, 2900022L05Rik, Dnhd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Ints10
Name: integrator complex subunit 10
Synonyms: 4921521J11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 70885
Homologene: 10029
Slc4a5
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 5
Synonyms: C330016K18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232156
Homologene: 31125
C8a
Name: complement component 8, alpha polypeptide
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230558
HGNC: HGNC:1352
Homologene: 472
Unc79
Name: unc-79 homolog
Synonyms: Mlca3, 9030205A07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217843
Homologene: 41397
Olfr615
Name: olfactory receptor 615
Synonyms: MOR19-2, GA_x6K02T2PBJ9-6284902-6285843
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259084
Homologene: 17494
4930562C15Rik
Name: RIKEN cDNA 4930562C15 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 78809
Homologene: 53527
Arsa
Name: arylsulfatase A
Synonyms: As2, ASA, As-2, AS-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11883
HGNC: HGNC:713
Homologene: 20138
Kmt2d
Name: lysine (K)-specific methyltransferase 2D
Synonyms: Mll2, C430014K11Rik, Mll4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 381022
HGNC: HGNC:7133
Homologene: 86893
Vmn2r104
Name: vomeronasal 2, receptor 104
Synonyms: V2r7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22313
Homologene: 129751
Nnt
Name: nicotinamide nucleotide transhydrogenase
Synonyms: 4930423F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18115
HGNC: HGNC:7863
Shpk
Name: sedoheptulokinase
Synonyms: Carkl, 4930431K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74637
HGNC: HGNC:1492
Homologene: 8319
Adgrg6
Name: adhesion G protein-coupled receptor G6
Synonyms: DREG, Gpr126, 1190004A11Rik, LOC215798
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215798
Homologene: 10724
Mast1
Name: microtubule associated serine/threonine kinase 1
Synonyms: SAST170, 9430008B02Rik, SAST
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 56527
Homologene: 10543
Nat3
Name: N-acetyltransferase 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17962
Homologene: 69026
Ica1l
Name: islet cell autoantigen 1-like
Synonyms: Als2cr15, 1700030B17Rik, b2b3465Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 70375
Homologene: 16323
Vmn1r30
Name: vomeronasal 1 receptor 30
Synonyms: V1rc9, V1rc22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 171195
Homologene: 137652
Tcp11l1
Name: t-complex 11 like 1
Synonyms: C130096D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320554
Homologene: 41260
Tas1r2
Name: taste receptor, type 1, member 2
Synonyms: TR2, Gpr71, T1r2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 83770
Homologene: 75323
Brsk2
Name: BR serine/threonine kinase 2
Synonyms: SAD-A, 4833424K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 75770
Homologene: 123829
Msi1
Name: musashi RNA-binding protein 1
Synonyms: Msi1h, Musahi1, m-Msi-1, Msi1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17690
HGNC: HGNC:7330
Homologene: 55657
Pik3c2b
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta
Synonyms: C330011J12Rik, PI3K-C2beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240752
HGNC: HGNC:8972
Homologene: 20582
Zhx3
Name: zinc fingers and homeoboxes 3
Synonyms: 1810059C13Rik, 4932418O04Rik, Tix1, 9530010N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320799
Homologene: 9000
Kng1
Name: kininogen 1
Synonyms: H-kininigen, L-kininogen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16644
HGNC: HGNC:6383
Homologene: 88343
Spesp1
Name: sperm equatorial segment protein 1
Synonyms: 4921508E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66712
VEGA: 9
Homologene: 49838
Rhd
Name: Rh blood group, D antigen
Synonyms: Rh, Rhced, Rhl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 19746
Homologene: 7918
Ccdc177
Name: coiled-coil domain containing 177
Synonyms: Gm1568, LOC380768
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 380768
VEGA: 12
Homologene: 128326
Prodh2
Name: proline dehydrogenase (oxidase) 2
Synonyms: POX1, 2510028N04Rik, 2510038B11Rik, MmPOX1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 56189
Homologene: 36381
Cpa2
Name: carboxypeptidase A2, pancreatic
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232680
HGNC: HGNC:2297
Homologene: 37541
Olfr552
Name: olfactory receptor 552
Synonyms: GA_x6K02T2PBJ9-5323062-5324015, MOR28-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259106
Homologene: 73941
Phactr3
Name: phosphatase and actin regulator 3
Synonyms: 4930415A02Rik, 1500003N10Rik, scapinin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74189
Homologene: 14482
Gm7003
Name: predicted gene 7003
Synonyms: Ighv(III)-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 629785
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 20,814,437 bp
  • T to C, chromosome 1 at 60,013,851 bp
  • C to T, chromosome 1 at 62,747,211 bp
  • C to A, chromosome 1 at 65,235,033 bp
  • C to T, chromosome 1 at 133,099,702 bp
  • T to C, chromosome 2 at 104,688,511 bp
  • A to G, chromosome 2 at 160,780,018 bp
  • A to G, chromosome 2 at 172,445,332 bp
  • T to A, chromosome 2 at 178,278,901 bp
  • T to C, chromosome 4 at 104,815,845 bp
  • T to C, chromosome 4 at 132,334,186 bp
  • G to A, chromosome 4 at 134,884,617 bp
  • C to A, chromosome 4 at 139,660,009 bp
  • A to T, chromosome 5 at 38,299,715 bp
  • A to T, chromosome 5 at 109,736,777 bp
  • G to A, chromosome 5 at 110,676,684 bp
  • T to C, chromosome 5 at 115,441,391 bp
  • T to C, chromosome 5 at 125,027,113 bp
  • A to G, chromosome 6 at 30,544,181 bp
  • A to T, chromosome 6 at 58,435,774 bp
  • T to C, chromosome 6 at 83,285,854 bp
  • G to T, chromosome 7 at 30,494,523 bp
  • G to A, chromosome 7 at 102,604,408 bp
  • T to A, chromosome 7 at 103,560,573 bp
  • T to C, chromosome 7 at 141,987,906 bp
  • T to C, chromosome 8 at 67,547,862 bp
  • G to A, chromosome 8 at 68,794,041 bp
  • G to T, chromosome 8 at 84,912,259 bp
  • A to G, chromosome 8 at 110,519,231 bp
  • T to C, chromosome 8 at 119,956,667 bp
  • A to T, chromosome 9 at 62,272,732 bp
  • C to T, chromosome 9 at 88,569,484 bp
  • T to A, chromosome 9 at 103,315,196 bp
  • T to C, chromosome 9 at 106,288,010 bp
  • A to T, chromosome 10 at 14,420,504 bp
  • T to A, chromosome 11 at 35,867,372 bp
  • T to C, chromosome 11 at 54,910,799 bp
  • A to G, chromosome 11 at 60,794,899 bp
  • C to T, chromosome 11 at 69,473,351 bp
  • T to C, chromosome 11 at 73,199,535 bp
  • T to C, chromosome 11 at 107,061,764 bp
  • G to A, chromosome 12 at 80,758,042 bp
  • C to T, chromosome 12 at 103,112,138 bp
  • A to G, chromosome 12 at 113,803,227 bp
  • T to C, chromosome 12 at 119,168,005 bp
  • C to T, chromosome 13 at 30,925,755 bp
  • C to T, chromosome 13 at 119,368,595 bp
  • T to C, chromosome 15 at 89,473,275 bp
  • G to A, chromosome 15 at 98,852,109 bp
  • G to A, chromosome 16 at 4,864,363 bp
  • A to G, chromosome 16 at 23,079,137 bp
  • T to A, chromosome 17 at 20,030,081 bp
  • T to G, chromosome 17 at 78,237,807 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5461 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
043023-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.