Strain Name:
C57BL/6J-MtgxR5627Btlr/Mmmh
Stock Number:
043166-MU
Citation ID:
RRID:MMRRC_043166-MU
Other Names:
R5627 (G1), C57BL/6J-MtgxR5627Btlr
Major Collection:

Strain Information

Slc6a5
Name: solute carrier family 6 (neurotransmitter transporter, glycine), member 5
Synonyms: Glyt2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 104245
Homologene: 37901
Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 77595
Homologene: 28122
Nbea
Name: neurobeachin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 26422
HGNC: HGNC:7648
Homologene: 69190
Eif4g2
Name: eukaryotic translation initiation factor 4, gamma 2
Synonyms: Nat1, DAP-5, Natm1, E130105L11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13690
HGNC: HGNC:3297
Homologene: 37477
Cep97
Name: centrosomal protein 97
Synonyms: 4932439K18Rik, 2810403B08Rik, E130116N02Rik, Lrriq2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74201
Homologene: 11579
Supt20
Name: SPT20 SAGA complex component
Synonyms: p38 interacting protein, p38IP, D3Ertd300e, Fam48a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56790
Homologene: 134155
Cep85
Name: centrosomal protein 85
Synonyms: 2410030J07Rik, Ccdc21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 70012
Homologene: 11254
Abl1
Name: c-abl oncogene 1, non-receptor tyrosine kinase
Synonyms: c-Abl, E430008G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11350
HGNC: HGNC:76
Homologene: 3783
Abcb8
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 8
Synonyms: 4833412N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 74610
HGNC: HGNC:49
Homologene: 5203
Nckap5l
Name: NCK-associated protein 5-like
Synonyms: C230021P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 380969
Homologene: 18924
Zfp318
Name: zinc finger protein 318
Synonyms: TZF, 2610034E08Rik, D530032D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 57908
Homologene: 22808
Klhl12
Name: kelch-like 12
Synonyms: C3ip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240756
Homologene: 23317
Rcc1
Name: regulator of chromosome condensation 1
Synonyms: 4931417M11Rik, Chc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100088
HGNC: HGNC:1913
Homologene: 55567
Cenpe
Name: centromere protein E
Synonyms: N-7 kinesin, CENP-E, 312kDa, Kif10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229841
HGNC: HGNC:1856
Homologene: 20429
Wdr36
Name: WD repeat domain 36
Synonyms: 5730444A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225348
VEGA: 18
Homologene: 6536
Inpp4a
Name: inositol polyphosphate-4-phosphatase, type I
Synonyms: 107kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269180
HGNC: HGNC:6074
Homologene: 2871
Golga2
Name: golgi autoantigen, golgin subfamily a, 2
Synonyms: GM130
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99412
Homologene: 3300
Tecpr2
Name: tectonin beta-propeller repeat containing 2
Synonyms: 4930573I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104859
VEGA: 12
Homologene: 8897
Rfx7
Name: regulatory factor X, 7
Synonyms: 2510005N23Rik, 9930116O05Rik, D130086K05Rik, Rfxdc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319758
Homologene: 11275
Atad2b
Name: ATPase family, AAA domain containing 2B
Synonyms: 1110014E10Rik, D530031C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 320817
VEGA: 12
Homologene: 86351
Slc38a6
Name: solute carrier family 38, member 6
Synonyms: EG625098
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 625098
Homologene: 27550
Egfem1
Name: EGF-like and EMI domain containing 1
Synonyms: 6130401L20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 75740
Homologene: 135950
Ccdc73
Name: coiled-coil domain containing 73
Synonyms: 2210415I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 211936
Homologene: 52235
Gm5134
Name: predicted gene 5134
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 333669
Homologene: 129891
Ces1f
Name: carboxylesterase 1F
Synonyms: TGH-2, CesML1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234564
HGNC: HGNC:1863
Homologene: 134311
Or11g26
Name: olfactory receptor family 11 subfamily G member 26
Synonyms: GA_x6K02T2PMLR-6224293-6225228, MOR106-6, Olfr742
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258422
Homologene: 74163
Col22a1
Name: collagen, type XXII, alpha 1
Synonyms: C80743, 2310067L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 69700
Homologene: 43567
Or5b96
Name: olfactory receptor family 5 subfamily B member 96
Synonyms: GA_x6K02T2RE5P-3220047-3219130, MOR202-2, Olfr1446
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258699
HGNC: HGNC:8324
Homologene: 74233
Serpinb9d
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9d
Synonyms: Spi9, AT2, ovalbumin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20726
HGNC: HGNC:8955
Homologene: 69093
Cyp3a59
Name: cytochrome P450, family 3, subfamily a, polypeptide 59
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100041449
Homologene: 135775
Kcnt2
Name: potassium channel, subfamily T, member 2
Synonyms: E330038N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240776
Homologene: 16121
Chil5
Name: chitinase-like 5
Synonyms: Bclp1, Bclp2, Chi3l7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229687
Homologene: 132668
Or14c39
Name: olfactory receptor family 14 subfamily C member 39
Synonyms: GA_x6K02T2NHDJ-9425121-9424195, MOR220-2, Olfr292
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258613
Homologene: 115526
Mtmr10
Name: myotubularin related protein 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233315
Homologene: 9822
Ano3
Name: anoctamin 3
Synonyms: B230324K02Rik, Tmem16c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228432
Homologene: 57147
Fam98b
Name: family with sequence similarity 98, member B
Synonyms: 2610510H03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68215
Homologene: 72241
Map3k3
Name: mitogen-activated protein kinase kinase kinase 3
Synonyms: MAPKKK3, Mekk3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 26406
HGNC: HGNC:6855
Homologene: 69110
Alpk1
Name: alpha-kinase 1
Synonyms: 8430410J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71481
Homologene: 11849
Or56b2
Name: olfactory receptor family 56 subfamily B member 2
Synonyms: GA_x6K02T2PBJ9-7316375-7317334, MOR40-4, Olfr661
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258743
Homologene: 17376
Or5g25
Name: olfactory receptor family 5 subfamily G member 25
Synonyms: GA_x6K02T2Q125-47127746-47126790, MOR175-2, Olfr1002
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258566
Homologene: 104131
Saraf
Name: store-operated calcium entry-associated regulatory factor
Synonyms: 1810045K07Rik, Tmem66
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67887
Homologene: 9398
Gm5150
Name: predicted gene 5150
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 381484
Homologene: 129516
Or4a70
Name: olfactory receptor family 4 subfamily A member 70
Synonyms: GA_x6K02T2Q125-50937307-50936387, MOR231-5, Olfr1242
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258970
Homologene: 27316
Rpl7a-ps8
Name: ribosomal protein L7A, pseudogene 8
Synonyms: Gm4970
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243872
Gm15475
Name: predicted gene 15475
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Gm3149
Name: predicted gene 3149
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Homologene: 115686
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 37,367,773 bp
  • T to C, chromosome 1 at 134,458,452 bp
  • A to G, chromosome 1 at 140,450,276 bp
  • T to C, chromosome 2 at 31,800,583 bp
  • C to A, chromosome 2 at 32,306,047 bp
  • T to A, chromosome 2 at 85,647,647 bp
  • A to T, chromosome 2 at 89,494,044 bp
  • T to C, chromosome 2 at 105,017,779 bp
  • T to C, chromosome 2 at 110,756,953 bp
  • T to C, chromosome 2 at 117,267,933 bp
  • A to G, chromosome 3 at 15,963,400 bp
  • G to T, chromosome 3 at 29,668,399 bp
  • A to G, chromosome 3 at 54,713,190 bp
  • C to T, chromosome 3 at 55,992,345 bp
  • A to T, chromosome 3 at 90,144,250 bp
  • A to G, chromosome 3 at 106,019,635 bp
  • A to T, chromosome 3 at 127,680,647 bp
  • G to T, chromosome 3 at 135,235,473 bp
  • T to C, chromosome 4 at 132,338,143 bp
  • A to T, chromosome 4 at 134,134,097 bp
  • C to T, chromosome 5 at 24,400,139 bp
  • T to A, chromosome 5 at 146,112,854 bp
  • C to T, chromosome 6 at 84,101,967 bp
  • G to T, chromosome 7 at 19,954,433 bp
  • A to G, chromosome 7 at 49,911,774 bp
  • A to T, chromosome 7 at 64,336,752 bp
  • G to T, chromosome 7 at 86,695,139 bp
  • G to A, chromosome 7 at 104,688,170 bp
  • A to G, chromosome 7 at 111,074,239 bp
  • T to A, chromosome 8 at 34,154,645 bp
  • A to T, chromosome 8 at 93,279,699 bp
  • C to T, chromosome 9 at 72,532,784 bp
  • A to G, chromosome 10 at 75,986,108 bp
  • A to G, chromosome 11 at 106,148,602 bp
  • A to G, chromosome 12 at 4,917,911 bp
  • A to G, chromosome 12 at 73,343,683 bp
  • T to A, chromosome 12 at 110,941,482 bp
  • C to T, chromosome 13 at 33,202,693 bp
  • CAAAA to CAA, chromosome 13 at 89,691,135 bp
  • A to T, chromosome 14 at 4,324,703 bp
  • A to G, chromosome 14 at 50,515,800 bp
  • T to C, chromosome 15 at 71,981,918 bp
  • A to T, chromosome 15 at 99,427,706 bp
  • A to G, chromosome 16 at 55,924,967 bp
  • T to C, chromosome 17 at 46,413,136 bp
  • T to A, chromosome 18 at 32,861,638 bp
  • T to A, chromosome 19 at 12,890,299 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5627 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043166-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.