Strain Name:
C57BL/6JMlcr-ApcMin/Mmmh
Stock Number:
043849-MU
Citation ID:
RRID:MMRRC_043849-MU
Other Names:
C57BL/6JD-ApcMin, Apc-Min, Min, MinD

Gene Information

Allele: ApcMin (Mus musculus (mouse))
Name: adenomatosis polyposis coli; multiple intestinal neoplasia
Synonyms: Apc<Δ850>, Min, Apc, Apc<->, Min-
Chromosome: 18
Alteration at locus: Chemically Induced
Monarch Initiative (Disease and Phenotype Associations): MGI:1856318
Gene: Apc (Mus musculus (mouse))
Name: adenomatosis polyposis coli
Synonyms: CC1, Min
Chromosome: 18
Alteration at locus: Chemically Induced
Monarch Initiative (Disease and Phenotype Associations): MGI:88039
Genetic Alterations

Chemically-induced point mutation in the Apc gene (MGI:88039) causing a truncation in the Apc protein. 

  • mRNA RefSeq: NM_007462.3
  • nucleic acid level: c.2549T>A
  • amino acid level (predicted): p.Leu850*
The strain was closed to outside B6 strains in the early 2000s and has been maintained as a closed colony. It carries approx. 308 private homozygous variants that distinguish it from the original JAX line (Jackson Strain #002020).
ES Cell Line
N/A
Phenotype

Homozygous: lethal

Hetero/Hemizygous: intestinal neoplasia, adenomas develop primarily in the small intestine with the preponderance of the adenomas developing in the distal have (illium) of small intestine.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
    Strain Development
    The strain was closed to outside B6 strains in the early 2000s and has been maintained as a closed colony.
    Suggested Control Mice
    Wild-type littermates
    • Cancer
    • Models for Human Disease
    Donor
    William Dove, Ph.D., McArdle Laboratory for Cancer Research.
    James Amos-Landgraf, Ph.D., Unviersity of Missouri.

    Colony and Husbandry Informationon

    Animals are maintained on breeder chow (5058 PicoLab Mouse Diet 20).

    Colony Surveillance Program and Current Health Reports

    For more information about this colony's health status contact mmrrc@missouri.edu
    Coat Color
    Black
    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    Progeny testing of breeder males to maintain only high tumor average offspring.
    Generation
    N20+F30+
    Overall Breeding Performance
    Good
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: No No
    Homozygotes are fertile: No No
    Heterozygotes are fertile: Reduced Yes
    Age Reproductive Decline: Less than 4 months Less than 4 months
    Bred to Homozygosity
    No
    Average litter size
    4-6
    Recommended wean age
    3-4 weeks
    Average Pups Weaned
    4-6

    Order Request Information

    Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@missouri.edu for more details.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    043849-MU-HET-F
    043849-MU-HET-M
    043849-MU-WT-F
    043849-MU-WT-M
    Heterozygous / Hemizygous Female
    Heterozygous / Hemizygous Male
    Wild Type Female
    Wild Type Male
    $290.00 / $590.00
    Non-Profit / For-Profit
    Per Mouse The mmrrc@missouri.edu may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
    043849-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity3 : 20-40 embryos / aliquot

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.