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Strain Detail Sheet

Strain Name:
C57BL/6JMlcr-ApcMin/Mmmh (GM4)
Stock Number:
050543-MU
Citation ID:
RRID:MMRRC_050543-MU
Other Names:
C57BL/6JD-ApcMin, Apc-Min, Min, MinD, GM4

Strain Information

Gene Details

ApcMin
Name: APC, WNT signaling pathway regulator; multiple intestinal neoplasia
Synonyms: Min, Apcdelta850, Min-, ApcΔ850, Apc580S, Apc-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
Apc
Name: APC, WNT signaling pathway regulator
Synonyms: Min, CC1, adenomatosis polyposis coli
Type: Gene
Species: Mouse
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 11789
HGNC: HGNC:583
Homologene: 30950

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations

Chemically-induced point mutation in the Apc gene (MGI:88039) causing a truncation in the Apc protein.

HGVS nomenclature:

  • Genbank RefSeq - mRNA: NM_001360980.1 (longest transcript)
  • Genbank RefSeq, protein: NP_001347909.1
  • Variant, nucleic acid level: c.2603T>A
  • Variant, amino acid level, predicted: p.Leu868*
  • Notes: Positionally crossreferenced against actual sequence (5'-cacaagcagaatctttatgg-3', Fig. 2A sequencing primer in PMID:1350108). This sequence was listed as 2365-to-2384, which converts to 2419-to-2438 in NM_001360980.1. An alternate description, using the PMID:1350108 positioning, requires reference to a shorter transcript:
    • Genbank RefSeq - mRNA: NM_007462.3
    • Variant, nucleic acid level: c.2549T>A
    • Variant, amino acid level, predicted: p.Leu850*
The strain was closed to outside B6 strains in the early 2000s and has been maintained as a closed colony. It carries approx. 300 private homozygous variants that distinguish it from the original JAX line (JAX Strain #002020).

MMRRC:43849 and MMRRC:50543 are genetically identical but harbor two distinct complex microbiota that alter the intestinal adenoma phenotype. See Microbiota Considerations (hyperlink to this) for more information.
Genotype Determination

Strain Description

Phenotype

Homozygous: lethal

Hetero/Hemizygous: intestinal neoplasia, adenomas develop primarily in the small intestine with the preponderance of the adenomas developing in the distal half. MMRRC:43849 and MMRRC:50543 are genetically identical but harbor two distinct complex microbiota that alter the intestinal adenoma phenotype. The original ApcMin mice from the closed genetic colony from William Dove at the University of Wisconsin was rederived using surrogate dams that harbored two distinct microbiota populations: low complexity and high complexity. The original closed colony line is available through the MMRRC with either the low complexity (MMRRC:43849) or high complexity (MMRRC:50543) microbiota that have the represented phenotypes in the small intestine and colon.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
MeSH Terms
  • Adenomatous Polyposis Coli/genetics
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Colorectal Neoplasms/genetics
  • DNA, Neoplasm/chemistry
  • DNA, Neoplasm/genetics
  • Genes, Tumor Suppressor
  • Genetic Linkage
  • Humans
  • Intestinal Neoplasms/genetics
  • Mice
  • Mice, Inbred AKR
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • Polymorphism, Restriction Fragment Length
  • Sequence Homology, Nucleic Acid
Strain Development
The strain was closed to outside B6 strains in the early 2000s and has been maintained as a closed colony. The original ApcMin mice from the closed genetic colony from William Dove at the University of Wisconsin was rederived using surrogate dams that harbored two distinct microbiota populations: low complexity and high complexity (PMID:29973630).

Rigor and Reproducibility Considerations (e.g. background strain genetics, microbiota characterization and influence)

MMRRC:43849 and MMRRC:50543 are genetically identical but harbor two distinct complex microbiota that alter the intestinal adenoma phenotype. The original ApcMin mice from the closed genetic colony from William Dove at the University of Wisconsin was rederived using surrogate dams that harbored two distinct microbiota populations: low complexity and high complexity (PMID:29973630). The original closed colony line is available through the MMRRC with either the low complexity (similar to JAX microbiota) or high complexity (similar to Envigo) microbiota that have the represented phenotypes in the small intestine (A) and colon (B).

Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Cancer
  • Models for Human Disease
  • Influence of Microbiota on Cancer Initiation and Development
  • Cancer Prevention
  • Drug Discovery
  • Cancer Metabolomics
  • Biomarker Discovery
  • Chemotherapeutics

Strain Origin

Donor
James Amos-Landgraf, Unviersity of Missouri.
William Dove, Ph.D., McArdle Laboratory for Cancer Research.
Primary Reference

Su LK, Kinzler KW, Vogelstein B, Preisinger AC, Moser AR, Luongo C, Gould KA, Dove WF. Multiple intestinal neoplasia caused by a mutation in the murine homologof the APC gene. Science. 1992 May 1;256(5057):668-70. Erratum in: Science 1992May 22;256(5060):1114. (Medline PMID: 1350108)

Hart ML, Ericsson AC, Lloyd KCK, Grimsrud KN, Rogala AR, Godfrey VL, NielsenJN, Franklin CL. Development of outbred CD1 mouse colonies with distinctstandardized gut microbiota profiles for use in complex microbiota targetedstudies. Sci Rep. 2018 Jul 4;8(1):10107. doi: 10.1038/s41598-018-28448-0. (Medline PMID: 29973630)

Colony and Husbandry Information

Special Considerations

The original ApcMin mice from the closed genetic colony from William Dove at the University of Wisconsin was rederived using surrogate dams that harbored two distinct microbiota populations: low complexity and high complexity. The original closed colony line is available through the MMRRC with either the low complexity (MMRRC:43849) or high complexity (MMRRC:50543) microbiota that have the represented phenotypes in the small intestine and colon. Crossing, co-housing, or mating to other mouse lines will potentially alter the microbiota and phenotype of this line. Littermate controls should be used to ensure appropriate scientific rigorous results.
Animals are maintained on breeder chow (5058 PicoLab Mouse Diet 20).

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black
Other
Rigor and Reproducibility Considerations (e.g. background strain genetics, microbiota characterization and influence)

MMRRC:43849 and MMRRC:50543 are genetically identical but harbor two distinct complex microbiota that alter the intestinal adenoma phenotype. The original ApcMin mice from the closed genetic colony from William Dove at the University of Wisconsin was rederived using surrogate dams that harbored two distinct microbiota populations: low complexity and high complexity (PMID:29973630). The original closed colony line is available through the MMRRC with either the low complexity (similar to JAX microbiota) or high complexity (similar to Envigo) microbiota that have the represented phenotypes in the small intestine (A) and colon (B).

* p < 0.05
** p < 0.01
*** p < 0.001

Statistical testing demonstrates that both MMRRC:43849 and MMRRC:50543 develop significantly more tumors than ApcMinmice from the Jackson Laboratory (Strain #002020). MMRRC:50543 (high complexity microbiota) develop significantly more tumors than MMRRC:43849 (low complexity microbiota). While MMRRC:50543 has similar tumor numbers to ApcMinmice from McCardle Laboratory for Cancer Research, MMRRC:43849 has significantly fewer tumors than ApcMin from McCardle.

Breeding

MMRRC Breeding System
Progeny testing of breeder males to maintain only high-tumor average offspring.
Generation
N20+F30+
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Reduced Yes
Age Reproductive Decline: less than 4 months less than 4 months
Bred to Homozygosity
No
Average litter size
4-6
Recommended wean age
3-4 weeks
Average Pups Weaned
4-6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Chase Bunger.

Conditions of Distribution

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Chase Bunger

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
050543-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit		 Aliquot Approximate quantity2 : 20-40 embryos / aliquot
050543-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit		 Aliquot Approximate quantity3
050543-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.