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Strain Name:
C57BL/6J-MtgxR7461Btlr/Mmmh
Stock Number:
045535-MU
Citation ID:
RRID:MMRRC_045535-MU
Other Names:
R7461 (G1)
Major Collection:

Strain Information

Arhgap10
Name: Rho GTPase activating protein 10
Synonyms: PSGAP-s, PSGAP-m, A930033B01Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 78514
Homologene: 12695
Gjd2
Name: gap junction protein, delta 2
Synonyms: connexin36, Cx36, Gja9
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14617
Homologene: 7734
Top1
Name: topoisomerase (DNA) I
Synonyms: Top-1, D130064I21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21969
Homologene: 2467
Por
Name: cytochrome p450 oxidoreductase
Synonyms: NADH cytochrome P450 oxydoreductase, CYPOR, CPR, 4933424M13Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18984
HGNC: HGNC:9208
Homologene: 725
Exoc2
Name: exocyst complex component 2
Synonyms: Sec5, 2410030I24Rik, Sec5l1, Gm29675
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66482
Homologene: 10122
Foxn2
Name: forkhead box N2
Synonyms: HTLF, Fkh19, 6030465J18Rik, 3230402J05Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14236
HGNC: HGNC:5281
Homologene: 31078
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 36,343,795 bp
  • G to T, chromosome 1 at 91,357,408 bp
  • G to T, chromosome 1 at 93,194,589 bp
  • T to A, chromosome 2 at 5,973,536 bp
  • T to C, chromosome 2 at 11,299,410 bp
  • A to T, chromosome 2 at 25,901,346 bp
  • A to G, chromosome 2 at 113,364,071 bp
  • A to C, chromosome 2 at 114,011,118 bp
  • A to T, chromosome 2 at 160,712,842 bp
  • T to A, chromosome 3 at 82,039,747 bp
  • C to T, chromosome 3 at 108,395,640 bp
  • T to C, chromosome 3 at 158,187,020 bp
  • A to G, chromosome 4 at 48,392,668 bp
  • A to G, chromosome 4 at 102,255,306 bp
  • T to C, chromosome 4 at 109,329,725 bp
  • A to T, chromosome 4 at 113,177,046 bp
  • CTATA to CTATATA, chromosome 4 at 130,021,213 bp
  • A to G, chromosome 5 at 30,266,928 bp
  • T to C, chromosome 5 at 105,031,014 bp
  • C to A, chromosome 5 at 111,224,129 bp
  • G to A, chromosome 5 at 122,818,865 bp
  • G to T, chromosome 5 at 135,729,504 bp
  • C to T, chromosome 6 at 30,508,470 bp
  • A to G, chromosome 7 at 27,637,170 bp
  • T to C, chromosome 8 at 77,388,697 bp
  • T to C, chromosome 8 at 105,260,564 bp
  • A to T, chromosome 9 at 27,334,122 bp
  • C to T, chromosome 9 at 65,053,120 bp
  • T to C, chromosome 10 at 36,989,236 bp
  • A to G, chromosome 11 at 60,505,152 bp
  • A to G, chromosome 11 at 65,218,603 bp
  • A to G, chromosome 11 at 69,548,990 bp
  • T to C, chromosome 11 at 71,759,973 bp
  • A to G, chromosome 11 at 100,011,939 bp
  • G to T, chromosome 11 at 103,616,290 bp
  • G to T, chromosome 11 at 115,786,256 bp
  • T to A, chromosome 12 at 69,756,461 bp
  • T to C, chromosome 12 at 113,144,157 bp
  • C to T, chromosome 13 at 30,882,272 bp
  • A to T, chromosome 14 at 30,066,163 bp
  • T to A, chromosome 14 at 41,643,276 bp
  • G to A, chromosome 14 at 56,527,044 bp
  • A to G, chromosome 14 at 62,481,089 bp
  • T to A, chromosome 14 at 123,827,799 bp
  • T to A, chromosome 15 at 78,527,349 bp
  • T to A, chromosome 17 at 30,988,057 bp
  • T to C, chromosome 17 at 43,627,926 bp
  • T to C, chromosome 17 at 88,486,883 bp
  • G to T, chromosome 18 at 57,252,853 bp
  • A to T, chromosome 18 at 71,306,034 bp
  • A to C, chromosome 18 at 78,856,492 bp
  • A to T, chromosome 19 at 12,861,777 bp
  • G to A, chromosome 19 at 34,252,531 bp
  • A to G, chromosome 19 at 47,982,122 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7461 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045535-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.