Strain Name:
C57BL/6J-MtgxR4544Btlr/Mmmh
Stock Number:
041779-MU
Citation ID:
RRID:MMRRC_041779-MU
Other Names:
R4544 (G1), C57BL/6J-MtgxR4544Btlr
Major Collection:

Strain Information

Pik3cb
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: 1110001J02Rik, p110beta
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74769
HGNC: HGNC:8976
Homologene: 21250
Fancd2
Name: Fanconi anemia, complementation group D2
Synonyms: 2410150O07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 211651
HGNC: HGNC:3585
Homologene: 13212
Atr
Name: ataxia telangiectasia and Rad3 related
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245000
HGNC: HGNC:882
Homologene: 96916
Sorbs1
Name: sorbin and SH3 domain containing 1
Synonyms: c-Cbl-associated protein, CAP, Sh3d5, 2310065E01Rik, 9530001P15Rik, Ponsin
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20411
VEGA: 19
Homologene: 83252
Myo9b
Name: myosin IXb
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 17925
HGNC: HGNC:7609
Homologene: 3058
Mfn2
Name: mitofusin 2
Synonyms: hypertension related protein 1, D630023P19Rik, Fzo
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 170731
Homologene: 8915
Ccnyl1
Name: cyclin Y-like 1
Synonyms: 9630037P07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227210
Homologene: 65050
Nek1
Name: NIMA (never in mitosis gene a)-related expressed kinase 1
Synonyms: kat, kidney, anemia and testis, D8Ertd790e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18004
HGNC: HGNC:7744
Homologene: 14376
Insig2
Name: insulin induced gene 2
Synonyms: Insig-2, 2900053I11Rik, C730043J18Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72999
Homologene: 9400
Lepr
Name: leptin receptor
Synonyms: Obr, leptin receptor gene-related protein, OB-RGRP, LEPROT, obl, obese-like, Modb1, Leprb
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16847
HGNC: HGNC:6554
Homologene: 1731
Ubr3
Name: ubiquitin protein ligase E3 component n-recognin 3
Synonyms: 4833421P10Rik, A130030D10Rik, 1110059H15Rik, Zfp650
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68795
Homologene: 52092
Atf7
Name: activating transcription factor 7
Synonyms: C130020M04Rik, 1110012F10Rik, 9430065F09Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223922
HGNC: HGNC:792
Homologene: 4994
Lsr
Name: lipolysis stimulated lipoprotein receptor
Synonyms: Lisch7, ILDR3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 54135
Homologene: 9306
Slc15a4
Name: solute carrier family 15, member 4
Synonyms: C130069N12Rik, PTR4, PHT1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100561
Homologene: 26432
Lmo2
Name: LIM domain only 2
Synonyms: Rbtn-2, Rbtn2, Rhom-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16909
HGNC: HGNC:6642
Homologene: 4072
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 94109
Homologene: 69536
Rdh9
Name: retinol dehydrogenase 9
Synonyms: Crad3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 103142
Homologene: 129514
Slc7a8
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 8
Synonyms: LAT2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 50934
VEGA: 14
Homologene: 8166
Ttn
Name: titin
Synonyms: connectin, L56, 1100001C23Rik, mdm, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Cmya5
Name: cardiomyopathy associated 5
Synonyms: Myospryn, 2310076E16Rik, 2310076E21Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 76469
VEGA: 13
Homologene: 137367
Zan
Name: zonadhesin
Synonyms: Zan
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22635
Homologene: 124417
Kdm7a
Name: lysine (K)-specific demethylase 7A
Synonyms: A630082K20Rik, ENSMUSG00000073143, Kdm7a, Jhdm1d
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 338523
Homologene: 25281
Vmn2r78
Name: vomeronasal 2, receptor 78
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 637896
Homologene: 115466
Slc8b1
Name: solute carrier family 8 (sodium/lithium/calcium exchanger), member B1
Synonyms: NCKX6, NCLX, Slc24a6
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 170756
Homologene: 41602
Klhl5
Name: kelch-like 5
Synonyms: 1300013C10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71778
HGNC: HGNC:6356
Homologene: 56736
Cspg4
Name: chondroitin sulfate proteoglycan 4
Synonyms: NG2, AN2, 4732461B14Rik, Cspg4a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 121021
VEGA: 9
HGNC: HGNC:2466
Homologene: 20445
Pde8a
Name: phosphodiesterase 8A
Synonyms: Pde8
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18584
HGNC: HGNC:8793
Homologene: 1957
Mndal
Name: myeloid nuclear differentiation antigen like
Synonyms: Ifi212
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 100040462
HGNC: HGNC:5395
Homologene: 115929
Osbpl6
Name: oxysterol binding protein-like 6
Synonyms: ORP-6, 1110062M20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99031
Homologene: 101447
Pex10
Name: peroxisomal biogenesis factor 10
Synonyms: LOC230983, peroxisome biogenesis factor 10
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 668173
HGNC: HGNC:8851
Homologene: 5671
Cmpk2
Name: cytidine/uridine monophosphate kinase 2
Synonyms: TDKI, 1200004E04Rik, Tyki
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 22169
VEGA: 12
Homologene: 10748
Mest
Name: mesoderm specific transcript
Synonyms: Peg1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17294
HGNC: HGNC:7028
Homologene: 1800
C1s1
Name: complement component 1, s subcomponent 1
Synonyms: C1s
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 50908
HGNC: HGNC:1247
Homologene: 1314
Vmn2r9
Name: vomeronasal 2, receptor 9
Synonyms: EG435864
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 435864
Homologene: 129606
Akr1c20
Name: aldo-keto reductase family 1, member C20
Synonyms: 2610528B18Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 116852
Ifi205
Name: interferon activated gene 205
Synonyms: D3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226695
Homologene: 115929
Psg26
Name: pregnancy-specific beta-1-glycoprotein 26
Synonyms: cea14, EG574429
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 574429
Homologene: 110989
Krt23
Name: keratin 23
Synonyms: K23, CK23, Krt1-23
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 94179
HGNC: HGNC:6438
Homologene: 9172
Fndc1
Name: fibronectin type III domain containing 1
Synonyms: 1110027O12Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 68655
Cd1d1
Name: CD1d1 antigen
Synonyms: CD1.1, Cd1a, Cd1d, Ly-38
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12479
HGNC: HGNC:1637
Homologene: 1337
Gm9934
Name: predicted gene 9934
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Prss56
Name: serine protease 56
Synonyms: Prss56, 1700027L20Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 69453
Homologene: 79885
Syne3
Name: spectrin repeat containing, nuclear envelope family member 3
Synonyms: nesprin-3alpha, nesprin-3, nesprin-3beta, 4831426I19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 212073
VEGA: 12
Homologene: 17625
Dppa3
Name: developmental pluripotency-associated 3
Synonyms: PGC7, 2410075G02Rik, stella
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73708
Homologene: 138189
Fastkd1
Name: FAST kinase domains 1
Synonyms: 5330408N05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320720
Homologene: 36420
Ifi213
Name: interferon activated gene 213
Synonyms: E030037K03Rik, Pydc4, Pyr-A
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 623121
HGNC: HGNC:5395
Homologene: 115929
Mkx
Name: mohawk homeobox
Synonyms: 9430023B20Rik, Irxl1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 210719
Homologene: 72239
Hdac1-ps
Name: histone deacetylase 1, pseudogene
Synonyms: EG15181, Gm10093
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15181
VEGA: 17
Alg9
Name: ALG9 alpha-1,2-mannosyltransferase
Synonyms: 8230402H15Rik, B430313H07Rik, Dibd1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 102580
Homologene: 6756
Chil4
Name: chitinase-like 4
Synonyms: Ym2, Chi3l4
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 104183
Homologene: 74931
Tas2r108
Name: taste receptor, type 2, member 108
Synonyms: T2R8, mGR08, mt2r8, T2r8, T2R108
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 57253
Homologene: 49480
Or2a12
Name: olfactory receptor family 2 subfamily A member 12
Synonyms: GA_x6K02T2P3E9-4632269-4631343, MOR261-12, Olfr446
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 258292
Homologene: 17179
Or6c5
Name: olfactory receptor family 6 subfamily C member 5
Synonyms: GA_x6K02T2PULF-10924432-10925370, MOR111-11, MOR111-2, Olfr774
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258232
Homologene: 105326
4933431K23Rik
Name: RIKEN cDNA 4933431K23 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74475
Uba52rt
Name: ubiquitin A-52 residue ribosomal protein fusion product 1, retrotransposed
Synonyms: Gm11808
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 666586
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 64,723,576 bp
  • A to G, chromosome 1 at 87,184,642 bp
  • A to T, chromosome 1 at 121,312,192 bp
  • T to C, chromosome 1 at 173,582,127 bp
  • A to T, chromosome 1 at 173,875,664 bp
  • T to C, chromosome 1 at 174,026,573 bp
  • C to T, chromosome 2 at 69,712,311 bp
  • G to A, chromosome 2 at 69,956,093 bp
  • T to C, chromosome 2 at 76,584,492 bp
  • T to C, chromosome 2 at 76,822,588 bp
  • C to T, chromosome 2 at 103,976,037 bp
  • T to C, chromosome 3 at 86,999,214 bp
  • C to A, chromosome 3 at 106,210,606 bp
  • C to T, chromosome 4 at 3,973,244 bp
  • G to A, chromosome 4 at 101,768,228 bp
  • A to G, chromosome 4 at 147,887,452 bp
  • T to C, chromosome 4 at 155,070,495 bp
  • C to T, chromosome 5 at 65,164,690 bp
  • T to G, chromosome 5 at 108,847,685 bp
  • G to A, chromosome 5 at 120,531,153 bp
  • A to G, chromosome 5 at 127,604,536 bp
  • C to G, chromosome 5 at 137,383,834 bp
  • T to C, chromosome 6 at 30,740,680 bp
  • T to C, chromosome 6 at 39,175,472 bp
  • A to G, chromosome 6 at 40,493,808 bp
  • C to T, chromosome 6 at 42,927,414 bp
  • A to G, chromosome 6 at 113,572,642 bp
  • A to G, chromosome 6 at 122,626,767 bp
  • A to G, chromosome 6 at 124,531,540 bp
  • T to C, chromosome 7 at 18,478,539 bp
  • C to T, chromosome 7 at 30,971,976 bp
  • A to T, chromosome 7 at 81,328,099 bp
  • A to T, chromosome 7 at 86,921,191 bp
  • A to G, chromosome 7 at 93,052,980 bp
  • A to T, chromosome 8 at 16,710,636 bp
  • C to T, chromosome 8 at 61,016,304 bp
  • T to C, chromosome 8 at 71,327,941 bp
  • A to G, chromosome 8 at 77,674,911 bp
  • C to T, chromosome 9 at 50,805,354 bp
  • T to A, chromosome 9 at 56,888,629 bp
  • A to T, chromosome 9 at 95,862,895 bp
  • T to A, chromosome 9 at 99,039,759 bp
  • T to C, chromosome 10 at 127,790,837 bp
  • A to C, chromosome 10 at 129,238,158 bp
  • G to A, chromosome 11 at 99,478,276 bp
  • A to G, chromosome 12 at 26,478,017 bp
  • T to A, chromosome 12 at 104,959,469 bp
  • A to C, chromosome 13 at 4,507,844 bp
  • G to A, chromosome 13 at 93,091,918 bp
  • C to G, chromosome 14 at 54,735,790 bp
  • A to G, chromosome 15 at 102,534,327 bp
  • T to A, chromosome 17 at 7,773,544 bp
  • A to T, chromosome 17 at 78,492,959 bp
  • T to C, chromosome 18 at 7,000,651 bp
  • G to A, chromosome 19 at 40,311,850 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4544 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041779-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.