Strain Name:
C57BL/6J-MtgxR5322Btlr/Mmmh
Stock Number:
042905-MU
Citation ID:
RRID:MMRRC_042905-MU
Other Names:
R5322 (G1), C57BL/6J-MtgxR5322Btlr
Major Collection:

Strain Information

Ppp2r2a
Name: protein phosphatase 2, regulatory subunit B, alpha
Synonyms: 2410004D02Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 71978
VEGA: 14
HGNC: HGNC:9304
Homologene: 2035
Kctd13
Name: potassium channel tetramerisation domain containing 13
Synonyms: 1500003N18Rik, Poldip1, Pdip1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233877
Homologene: 27800
Efr3a
Name: EFR3 homolog A
Synonyms: A130089M23Rik, D030063F01Rik, C920006C10Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 76740
Homologene: 44222
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Usp47
Name: ubiquitin specific peptidase 47
Synonyms: 4930502N04Rik, A630020C16Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74996
Homologene: 9929
Mast2
Name: microtubule associated serine/threonine kinase 2
Synonyms: MAST205, Mtssk
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17776
Homologene: 7428
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Mib1
Name: MIB E3 ubiquitin protein ligase 1
Synonyms: Mib, E430019M12Rik, skeletrophin, mind bomb-1, mindbomb
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225164
Homologene: 10810
Appbp2
Name: amyloid beta precursor protein binding protein 2
Synonyms: PAT1, 1300003O07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66884
HGNC: HGNC:622
Homologene: 31378
Trrap
Name: transformation/transcription domain-associated protein
Synonyms: transactivation/transformation-domain associated protein
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100683
Homologene: 39246
N4bp2
Name: NEDD4 binding protein 2
Synonyms: LOC386488, LOC333789, B3bp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 333789
Homologene: 32396
Enpp6
Name: ectonucleotide pyrophosphatase/phosphodiesterase 6
Synonyms: B830047L21Rik, D8Ertd514e, 4833421B01Rik, Npp6
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 320981
Homologene: 62657
Pi4kb
Name: phosphatidylinositol 4-kinase beta
Synonyms: ESTM41, Pik4cb
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 107650
HGNC: HGNC:8984
Homologene: 6741
Copb2
Name: COPI coat complex subunit beta 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 50797
HGNC: HGNC:2232
Homologene: 3499
Zc2hc1a
Name: zinc finger, C2HC-type containing 1A
Synonyms: 3110050N22Rik, Fam164a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67306
Homologene: 9338
Paxbp1
Name: PAX3 and PAX7 binding protein 1
Synonyms: 1810007M14Rik, Pax3/7bp, Gcfc1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 67367
Homologene: 9604
Patl1
Name: protein associated with topoisomerase II homolog 1 (yeast)
Synonyms: Pat1b
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225929
VEGA: 19
Homologene: 82269
Emc1
Name: ER membrane protein complex subunit 1
Synonyms: C230096C10Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230866
Homologene: 9002
Cachd1
Name: cache domain containing 1
Synonyms: 1190007F10Rik, Vwcd1, B430218L07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320508
Homologene: 10854
Moxd1
Name: monooxygenase, DBH-like 1
Synonyms: 3230402N08Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 59012
Homologene: 22904
Dcn
Name: decorin
Synonyms: DC, SLRR1B
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13179
VEGA: 10
HGNC: HGNC:2705
Homologene: 22430
Amy1
Name: amylase 1, salivary
Synonyms: Amy-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11722
Homologene: 20179
Cfi
Name: complement component factor i
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 12630
HGNC: HGNC:5394
Homologene: 171
Smco2
Name: single-pass membrane protein with coiled-coil domains 2
Synonyms: 1700023A16Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 69371
Homologene: 106211
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: Mdnah5, b2b1154Clo, b2b1134Clo, b2b1537Clo, b2b1565Clo, Dnahc5, b2b3491Clo
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Dnah10
Name: dynein, axonemal, heavy chain 10
Synonyms: Dnahc10
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56087
HGNC: HGNC:2941
Homologene: 25816
Helz2
Name: helicase with zinc finger 2, transcriptional coactivator
Synonyms: BC006779
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 229003
Homologene: 14118
Fbn2
Name: fibrillin 2
Synonyms: sy, Sne, Fib-2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 14119
VEGA: 18
HGNC: HGNC:3604
Homologene: 1515
Dock3
Name: dedicator of cyto-kinesis 3
Synonyms: PBP, Moca
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 208869
HGNC: HGNC:2989
Homologene: 21030
Crip1
Name: cysteine-rich protein 1
Synonyms: CRP1, Crip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 12925
HGNC: HGNC:2360
Homologene: 37465
Adamts3
Name: ADAM metallopeptidase with thrombospondin type 1 motif 3
Synonyms: 6330442E02Rik, 1100001H14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 330119
HGNC: HGNC:219
Homologene: 8596
Arvcf
Name: armadillo repeat gene deleted in velocardiofacial syndrome
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11877
HGNC: HGNC:728
Homologene: 31046
Ankar
Name: ankyrin and armadillo repeat containing
Synonyms: 4932422E22Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 319695
Homologene: 85163
Ankrd60
Name: ankyrin repeat domain 60
Synonyms: 1700019A24Rik, 1700030G11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 70065
Homologene: 43792
D2hgdh
Name: D-2-hydroxyglutarate dehydrogenase
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98314
Homologene: 5534
Tmem260
Name: transmembrane protein 260
Synonyms: 6720456H20Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218989
Homologene: 49506
Tex14
Name: testis expressed gene 14 intercellular bridge forming factor
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 83560
Homologene: 12838
Dipk2a
Name: divergent protein kinase domain 2A
Synonyms: GoPro49, 1190002N15Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 68861
Homologene: 18315
Speer4f1
Name: spermatogenesis associated glutamate (E)-rich protein 4F1
Synonyms: SPEER-4F, 4922502J04Rik, Speer4f
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 70935
Homologene: 69402
Myot
Name: myotilin
Synonyms: 5530402I04Rik, Ttid
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 58916
Homologene: 4942
Fignl1
Name: fidgetin-like 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 60530
Homologene: 11063
Pla2g3
Name: phospholipase A2, group III
Synonyms: 9130003P18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237625
Homologene: 9251
Sidt1
Name: SID1 transmembrane family, member 1
Synonyms: B830021E24Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 320007
Homologene: 41189
Fbxl12
Name: F-box and leucine-rich repeat protein 12
Synonyms: 3110048D16Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 30843
Homologene: 32198
Ckmt2
Name: creatine kinase, mitochondrial 2
Synonyms: 2300008A19Rik, ScCKmit
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 76722
HGNC: HGNC:1996
Homologene: 68206
Gbp10
Name: guanylate-binding protein 10
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 626578
Homologene: 128731
Kndc1
Name: kinase non-catalytic C-lobe domain (KIND) containing 1
Synonyms: VKIND, very-kind, 2410012C07Rik, B830014K08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76484
Homologene: 45138
Or52n2
Name: olfactory receptor family 52 subfamily N member 2
Synonyms: GA_x6K02T2PBJ9-7522449-7521493, MOR34-1, Olfr666
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259100
Homologene: 64956
Ccdc66
Name: coiled-coil domain containing 66
Synonyms: E230015L20Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 320234
VEGA: 14
Homologene: 35309
Or51b6b
Name: olfactory receptor family 51 subfamily B member 6B
Synonyms: GA_x6K02T2PBJ9-6384836-6383883, MOR1-4, Olfr623
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 259126
Homologene: 133591
Zeb2
Name: zinc finger E-box binding homeobox 2
Synonyms: SIP1, Zfx1b, 9130203F04Rik, D130016B08Rik, Zfhx1b
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 24136
Homologene: 8868
Or8b52
Name: olfactory receptor family 8 subfamily B member 52
Synonyms: GA_x6K02T2PVTD-32368166-32367237, MOR168-2P, Olfr917
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258183
VEGA: 9
Cnksr3
Name: Cnksr family member 3
Synonyms: Magi1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215748
Homologene: 18192
Rela
Name: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Synonyms: p65 NF kappaB, p65
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 19697
HGNC: HGNC:9955
Homologene: 32064
Or10ac1
Name: olfactory receptor family 10 subfamily AC member 1
Synonyms: GA_x6K02T2P3E9-5021913-5022918, MOR0-2P, MOR0-17_p, MORO-2, EG546896, Olfr455-ps1, Olfr455
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 546896
Homologene: 67366
Or8b3
Name: olfactory receptor family 8 subfamily B member 3
Synonyms: M3, GA_x6K02T2PVTD-32098059-32099003, MOR164-1, Olfr147
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258869
VEGA: 9
Homologene: 128279
Il1rn
Name: interleukin 1 receptor antagonist
Synonyms: IL-1ra, F630041P17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16181
HGNC: HGNC:6000
Homologene: 11163
Epha10
Name: Eph receptor A10
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230735
Homologene: 67804
Or52a20
Name: olfactory receptor family 52 subfamily A member 20
Synonyms: GA_x6K02T2L9TJ-1933-2295, GA_x6K02T2PBJ9-6440320-6440766, MOR22-4, Olfr627, Olfr243
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 436002
Homologene: 8420
Gpr157
Name: G protein-coupled receptor 157
Synonyms: F730108M23Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269604
Homologene: 18573
Or2ah1
Name: olfactory receptor family 2 subfamily AH member 1
Synonyms: GA_x6K02T2Q125-47301584-47302519, MOR260-5, Olfr1018
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258579
Homologene: 27216
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 72,690,386 bp
  • T to C, chromosome 1 at 93,829,898 bp
  • G to A, chromosome 2 at 24,348,629 bp
  • A to C, chromosome 2 at 44,997,095 bp
  • T to A, chromosome 2 at 85,823,187 bp
  • T to A, chromosome 2 at 173,568,817 bp
  • A to G, chromosome 2 at 181,234,005 bp
  • A to G, chromosome 3 at 7,551,421 bp
  • G to A, chromosome 3 at 94,994,249 bp
  • A to T, chromosome 3 at 113,561,859 bp
  • C to T, chromosome 3 at 129,873,040 bp
  • T to C, chromosome 4 at 100,952,122 bp
  • A to G, chromosome 4 at 116,333,411 bp
  • A to G, chromosome 4 at 124,885,748 bp
  • A to T, chromosome 4 at 139,354,246 bp
  • A to G, chromosome 4 at 150,098,852 bp
  • A to T, chromosome 5 at 17,477,349 bp
  • T to A, chromosome 5 at 65,790,457 bp
  • C to T, chromosome 5 at 89,707,300 bp
  • C to T, chromosome 5 at 105,217,481 bp
  • G to A, chromosome 5 at 124,773,566 bp
  • T to C, chromosome 5 at 144,844,224 bp
  • T to C, chromosome 6 at 42,539,016 bp
  • T to C, chromosome 6 at 146,871,287 bp
  • T to C, chromosome 7 at 103,660,672 bp
  • T to A, chromosome 7 at 103,717,112 bp
  • C to T, chromosome 7 at 104,893,164 bp
  • C to T, chromosome 7 at 112,053,269 bp
  • T to A, chromosome 7 at 126,929,206 bp
  • T to C, chromosome 7 at 139,936,809 bp
  • C to A, chromosome 8 at 47,068,915 bp
  • A to T, chromosome 9 at 20,639,008 bp
  • A to T, chromosome 9 at 38,403,566 bp
  • T to A, chromosome 9 at 38,665,206 bp
  • A to G, chromosome 9 at 94,520,562 bp
  • A to T, chromosome 9 at 98,585,976 bp
  • G to A, chromosome 9 at 106,901,829 bp
  • A to T, chromosome 10 at 7,135,078 bp
  • A to T, chromosome 10 at 24,244,253 bp
  • A to T, chromosome 10 at 97,517,602 bp
  • A to G, chromosome 11 at 3,488,686 bp
  • A to T, chromosome 11 at 11,801,571 bp
  • A to G, chromosome 11 at 85,196,064 bp
  • A to G, chromosome 11 at 87,511,472 bp
  • T to A, chromosome 12 at 113,153,597 bp
  • G to T, chromosome 13 at 91,861,772 bp
  • A to G, chromosome 14 at 27,482,527 bp
  • C to T, chromosome 14 at 48,486,849 bp
  • A to G, chromosome 14 at 67,038,873 bp
  • A to T, chromosome 14 at 67,770,266 bp
  • T to C, chromosome 14 at 103,185,683 bp
  • A to G, chromosome 15 at 28,384,244 bp
  • A to G, chromosome 15 at 65,788,336 bp
  • T to C, chromosome 16 at 18,397,643 bp
  • T to A, chromosome 16 at 44,281,622 bp
  • T to C, chromosome 16 at 91,015,162 bp
  • A to G, chromosome 18 at 10,792,975 bp
  • T to C, chromosome 18 at 44,354,149 bp
  • A to T, chromosome 18 at 58,039,315 bp
  • T to A, chromosome 19 at 5,645,380 bp
  • C to T, chromosome 19 at 11,920,859 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5322 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042905-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.