Strain Name:
C57BL/6J-MtgxR8999Btlr/Mmmh
Stock Number:
068830-MU
Citation ID:
RRID:MMRRC_068830-MU
Other Names:
R8999 (G1)
Major Collection:

Strain Information

Tyrp1
Name: tyrosinase-related protein 1
Synonyms: isa, TRP-1, Oca3, TRP1, Tyrp
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 22178
Homologene: 464
Gabrg1
Name: gamma-aminobutyric acid type A receptor subunit gamma 1
Synonyms: GabaA
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14405
HGNC: HGNC:4086
Homologene: 22570
Fxyd2
Name: FXYD domain-containing ion transport regulator 2
Synonyms: Atp1g1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11936
VEGA: 9
HGNC: HGNC:4026
Ptprf
Name: protein tyrosine phosphatase receptor type F
Synonyms: LAR, RPTP-LAR
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19268
HGNC: HGNC:9670
Homologene: 20623
Mfn1
Name: mitofusin 1
Synonyms: HR2, 6330416C07Rik, D3Ertd265e, 2310002F04Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 67414
Homologene: 11481
Plekha1
Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Synonyms: TAPP1, C920009D07Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101476
Homologene: 11001
Ddx10
Name: DEAD box helicase 10
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 10, 4632415A01Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 77591
VEGA: 9
HGNC: HGNC:2735
Homologene: 20922
Cep41
Name: centrosomal protein 41
Synonyms: Cep41, 2810431D15Rik, 1700017E11Rik, Tsga14
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 83922
Homologene: 10284
Supt5
Name: suppressor of Ty 5, DSIF elongation factor subunit
Synonyms: Supt5h, Spt5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20924
Homologene: 2384
Ptprc
Name: protein tyrosine phosphatase receptor type C
Synonyms: Lyt-4, CD45, B220, T200, Ly-5
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19264
HGNC: HGNC:9666
Homologene: 2126
Map2k3
Name: mitogen-activated protein kinase kinase 3
Synonyms: Prkmk3, MAP kinase kinase 3, MKK3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 26397
HGNC: HGNC:6843
Homologene: 56430
Ikzf1
Name: IKAROS family zinc finger 1
Synonyms: Ikaros, 5832432G11Rik, LyF-1, Zfpn1a1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22778
Homologene: 55948
Map2
Name: microtubule-associated protein 2
Synonyms: repro4, G1-397-34, Mtap2, MAP-2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17756
HGNC: HGNC:6839
Homologene: 1779
Mpdz
Name: multiple PDZ domain crumbs cell polarity complex component
Synonyms: B930003D11Rik, multiple PDZ domain protein, MUPP1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17475
HGNC: HGNC:7208
Homologene: 2841
Lbr
Name: lamin B receptor
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98386
HGNC: HGNC:6518
Homologene: 2455
Six3
Name: sine oculis-related homeobox 3
Synonyms: E130112M24Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20473
Homologene: 3947
Rbp3
Name: retinol binding protein 3, interstitial
Synonyms: Irbp, Rbp-3
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 19661
VEGA: 14
HGNC: HGNC:9921
Homologene: 9261
Dnah1
Name: dynein, axonemal, heavy chain 1
Synonyms: MDHC7, Dnahc1, B230373P09Rik, E030034C22Rik, ferf1, G1-415-19
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 110084
VEGA: 14
HGNC: HGNC:2940
Homologene: 67131
Nkx6-1
Name: NK6 homeobox 1
Synonyms: NKX6A, Nkx6.1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18096
HGNC: HGNC:7839
Homologene: 4495
Dsg3
Name: desmoglein 3
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13512
VEGA: 18
HGNC: HGNC:3050
Homologene: 55513
Tnik
Name: TRAF2 and NCK interacting kinase
Synonyms: 4831440I19Rik, C630040K21Rik, C530008O15Rik, 1500031A17Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 665113
Homologene: 77943
Dusp19
Name: dual specificity phosphatase 19
Synonyms: SKRP1, TS-DSP1, C79103, 5930436K22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68082
Homologene: 41565
Gm11639
Name: predicted gene 11639
Synonyms: Gm11639, Efcab15
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 105242472
Trim45
Name: tripartite motif-containing 45
Synonyms: 4921530N01Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229644
Homologene: 11865
Stk35
Name: serine/threonine kinase 35
Synonyms: CLP-36 interacting kinase, CLIK1, 1700054C12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67333
Homologene: 15441
Odad2
Name: outer dynein arm docking complex subunit 2
Synonyms: b2b227.1Clo, Armc4, b2b643Clo, 4930463I21Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 74934
VEGA: 18
Homologene: 9992
Krt18
Name: keratin 18
Synonyms: Krt1-18, Endo B, CK18, K18
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 16668
VEGA: 15
HGNC: HGNC:6430
Homologene: 55448
Prkch
Name: protein kinase C, eta
Synonyms: Pkch
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18755
HGNC: HGNC:9403
Homologene: 84384
Cfap52
Name: cilia and flagella associated protein 52
Synonyms: 4933417B11Rik, Wdr16, 1700019F09Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71860
Homologene: 12416
Mypn
Name: myopalladin
Synonyms: 1110056A04Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 68802
VEGA: 10
Homologene: 23778
Serpina6
Name: serine (or cysteine) peptidase inhibitor, clade A, member 6
Synonyms: Cbg
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 12401
HGNC: HGNC:1540
Homologene: 20417
Fap
Name: fibroblast activation protein
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14089
HGNC: HGNC:3590
Homologene: 48282
Notum
Name: notum palmitoleoyl-protein carboxylesterase
Synonyms: 5730593N15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 77583
Homologene: 45335
Men1
Name: multiple endocrine neoplasia 1
Synonyms: menin
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 17283
HGNC: HGNC:7010
Homologene: 7418
Glb1l3
Name: galactosidase, beta 1 like 3
Synonyms: 4921509F24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 70893
Homologene: 72240
Vmn2r69
Name: vomeronasal 2, receptor 69
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330581
Homologene: 115466
Or11i1
Name: olfactory receptor family 11 subfamily I member 1
Synonyms: MOR122-2, GA_x6K02T2N6GK-529983-529033, Olfr266
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 258482
Homologene: 51728
Pabpc4l
Name: poly(A) binding protein, cytoplasmic 4-like
Synonyms: C330050A14Rik, EG241989
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 241989
Homologene: 66336
Ppp2r2b
Name: protein phosphatase 2, regulatory subunit B, beta
Synonyms: PR55-BETA, PP2A-PR55B, 6330404L05Rik, 2900026H06Rik, E130009M08Rik, SCA12
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 72930
HGNC: HGNC:9305
Homologene: 55833
Or6c3
Name: olfactory receptor family 6 subfamily C member 3
Synonyms: Olfr788, GA_x6K02T2PULF-11151514-11152449, MOR111-4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 258544
Homologene: 74200
Or2b28
Name: olfactory receptor family 2 subfamily B member 28
Synonyms: Olfr1367, GA_x6K02T2QHY8-11899770-11898820, MOR256-65, MOR256-16
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 258526
Aipl1
Name: aryl hydrocarbon receptor-interacting protein-like 1
Synonyms: A930007I01Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 114230
HGNC: HGNC:359
Homologene: 22806
Msantd5f9
Name: Myb/SANT DNA binding domain containing 5 family member 9
Synonyms: Gm11756
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 623281
Cd6
Name: CD6 antigen
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12511
HGNC: HGNC:1691
Homologene: 4893
Igkv8-26
Name: immunoglobulin kappa variable 8-26
Synonyms: Gm16945
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 620240
HGNC: HGNC:5834
Pcdha2
Name: protocadherin alpha 2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 353234
HGNC: HGNC:8668
Homologene: 135719
Gpr27
Name: G protein-coupled receptor 27
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14761
HGNC: HGNC:4482
Homologene: 7344
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 66,413,314 bp
  • A to G, chromosome 1 at 138,101,192 bp
  • C to T, chromosome 1 at 181,818,947 bp
  • T to C, chromosome 2 at 62,537,024 bp
  • T to A, chromosome 2 at 80,630,927 bp
  • C to A, chromosome 2 at 129,810,589 bp
  • A to G, chromosome 3 at 28,665,771 bp
  • T to A, chromosome 3 at 32,569,534 bp
  • A to C, chromosome 3 at 46,446,348 bp
  • A to G, chromosome 3 at 100,931,644 bp
  • A to T, chromosome 3 at 106,821,683 bp
  • G to C, chromosome 4 at 73,919,183 bp
  • T to C, chromosome 4 at 80,844,857 bp
  • C to A, chromosome 4 at 81,284,645 bp
  • A to G, chromosome 4 at 118,226,474 bp
  • T to A, chromosome 5 at 70,816,035 bp
  • T to C, chromosome 5 at 101,664,216 bp
  • T to C, chromosome 6 at 30,666,166 bp
  • C to A, chromosome 6 at 70,193,530 bp
  • T to C, chromosome 6 at 99,692,815 bp
  • A to G, chromosome 7 at 28,338,423 bp
  • A to C, chromosome 7 at 85,411,099 bp
  • T to C, chromosome 7 at 130,908,469 bp
  • A to T, chromosome 9 at 26,853,618 bp
  • T to C, chromosome 9 at 45,402,997 bp
  • T to C, chromosome 9 at 53,229,234 bp
  • A to T, chromosome 10 at 63,162,271 bp
  • A to G, chromosome 10 at 129,473,517 bp
  • A to G, chromosome 11 at 11,685,013 bp
  • T to C, chromosome 11 at 60,949,991 bp
  • T to C, chromosome 11 at 67,927,311 bp
  • T to C, chromosome 11 at 72,030,257 bp
  • A to T, chromosome 11 at 104,749,651 bp
  • G to A, chromosome 11 at 120,654,381 bp
  • T to C, chromosome 12 at 73,696,199 bp
  • C to G, chromosome 12 at 103,651,729 bp
  • A to G, chromosome 13 at 21,347,818 bp
  • T to C, chromosome 14 at 31,296,278 bp
  • C to T, chromosome 14 at 33,962,403 bp
  • A to C, chromosome 15 at 102,031,439 bp
  • T to A, chromosome 17 at 85,623,736 bp
  • C to A, chromosome 18 at 7,211,574 bp
  • T to C, chromosome 18 at 20,533,627 bp
  • C to T, chromosome 18 at 36,940,375 bp
  • C to T, chromosome 18 at 42,737,928 bp
  • G to A, chromosome 19 at 6,339,930 bp
  • A to T, chromosome 19 at 10,799,278 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8999 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068830-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.