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Strain Name:
C57BL/6J-MtgxR2082Btlr/Mmmh
Stock Number:
040087-MU
Citation ID:
RRID:MMRRC_040087-MU
Other Names:
R2082 (G1), C57BL/6J-MtgxR2082Btlr
Major Collection:

Strain Information

Myo10
Name: myosin X
Synonyms: myosin-X, D15Ertd600e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17909
HGNC: HGNC:7593
Homologene: 36328
Vps41
Name: VPS41 HOPS complex subunit
Synonyms: Vam2, mVam2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218035
VEGA: 13
Homologene: 69165
P2rx7
Name: purinergic receptor P2X, ligand-gated ion channel, 7
Synonyms: P2X7 receptor, P2X7R, P2X(7)
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18439
HGNC: HGNC:8537
Homologene: 1925
Upf1
Name: UPF1 RNA helicase and ATPase
Synonyms: PNORF-1, B430202H16Rik, Rent1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19704
HGNC: HGNC:9962
Homologene: 2185
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Hsp90aa1
Name: heat shock protein 90, alpha (cytosolic), class A member 1
Synonyms: hsp4, Hsp90, Hsp86-1, Hsp89, Hspca
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 15519
Homologene: 68464
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 37,895,326 bp
  • T to G, chromosome 1 at 135,884,976 bp
  • C to T, chromosome 1 at 139,550,886 bp
  • T to A, chromosome 2 at 30,796,379 bp
  • A to G, chromosome 2 at 32,709,501 bp
  • T to C, chromosome 2 at 92,613,990 bp
  • G to T, chromosome 2 at 169,886,215 bp
  • A to T, chromosome 3 at 9,699,485 bp
  • T to C, chromosome 3 at 20,064,706 bp
  • C to T, chromosome 3 at 87,725,996 bp
  • G to A, chromosome 3 at 133,370,417 bp
  • A to G, chromosome 3 at 133,485,727 bp
  • A to G, chromosome 4 at 101,002,958 bp
  • A to G, chromosome 4 at 123,860,976 bp
  • G to A, chromosome 4 at 152,559,364 bp
  • A to T, chromosome 5 at 65,807,565 bp
  • A to T, chromosome 5 at 122,644,095 bp
  • A to T, chromosome 6 at 39,345,881 bp
  • A to T, chromosome 6 at 90,694,574 bp
  • T to C, chromosome 7 at 4,550,775 bp
  • C to A, chromosome 7 at 56,297,137 bp
  • C to T, chromosome 7 at 105,288,503 bp
  • T to A, chromosome 7 at 119,580,634 bp
  • C to A, chromosome 7 at 140,779,896 bp
  • T to C, chromosome 8 at 4,518,236 bp
  • T to A, chromosome 8 at 25,921,549 bp
  • T to C, chromosome 8 at 26,978,988 bp
  • A to T, chromosome 8 at 70,341,572 bp
  • T to C, chromosome 8 at 70,763,728 bp
  • A to T, chromosome 8 at 83,270,987 bp
  • A to T, chromosome 8 at 85,263,465 bp
  • T to C, chromosome 8 at 110,726,257 bp
  • C to T, chromosome 8 at 113,775,333 bp
  • G to A, chromosome 9 at 19,339,278 bp
  • G to A, chromosome 10 at 13,560,163 bp
  • G to A, chromosome 10 at 34,155,940 bp
  • A to G, chromosome 10 at 40,585,339 bp
  • A to G, chromosome 10 at 59,863,307 bp
  • A to G, chromosome 10 at 76,709,596 bp
  • G to T, chromosome 10 at 77,059,293 bp
  • A to G, chromosome 10 at 78,715,033 bp
  • A to T, chromosome 10 at 79,743,512 bp
  • T to C, chromosome 10 at 127,199,089 bp
  • T to C, chromosome 10 at 127,484,855 bp
  • T to A, chromosome 11 at 55,052,389 bp
  • A to T, chromosome 11 at 85,030,512 bp
  • A to G, chromosome 11 at 99,388,875 bp
  • G to T, chromosome 11 at 102,153,377 bp
  • T to C, chromosome 11 at 103,192,025 bp
  • T to A, chromosome 11 at 105,147,083 bp
  • T to A, chromosome 12 at 4,775,439 bp
  • A to T, chromosome 12 at 110,692,827 bp
  • T to G, chromosome 13 at 18,852,351 bp
  • C to A, chromosome 13 at 100,304,344 bp
  • C to A, chromosome 13 at 100,806,533 bp
  • T to C, chromosome 14 at 20,508,678 bp
  • T to C, chromosome 14 at 49,015,587 bp
  • T to A, chromosome 15 at 25,785,993 bp
  • T to A, chromosome 15 at 32,618,856 bp
  • T to C, chromosome 15 at 35,910,746 bp
  • T to C, chromosome 15 at 81,913,695 bp
  • G to A, chromosome 15 at 102,031,020 bp
  • A to G, chromosome 16 at 15,715,963 bp
  • T to C, chromosome 16 at 32,376,036 bp
  • T to A, chromosome 16 at 59,148,885 bp
  • A to G, chromosome 17 at 23,460,256 bp
  • C to A, chromosome 17 at 26,543,696 bp
  • T to C, chromosome 17 at 40,225,860 bp
  • A to T, chromosome 18 at 64,458,189 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2082 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040087-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.