Strain Name:
C57BL/6J-MtgxR5514Btlr/Mmmh
Stock Number:
043074-MU
Citation ID:
RRID:MMRRC_043074-MU
Other Names:
R5514 (G1), C57BL/6J-MtgxR5514Btlr
Major Collection:

Strain Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Sema7a
Name: sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A
Synonyms: Semaphorin K1, CDw108, Semal, 2900057C09Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20361
VEGA: 9
Homologene: 2678
Ppp1r1b
Name: protein phosphatase 1, regulatory inhibitor subunit 1B
Synonyms: DARPP-32, Darpp32
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 19049
HGNC: HGNC:9287
Homologene: 12972
Vldlr
Name: very low density lipoprotein receptor
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22359
Homologene: 443
Nmu
Name: neuromedin U
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56183
HGNC: HGNC:7859
Homologene: 4863
Upp1
Name: uridine phosphorylase 1
Synonyms: UdRPase, UPase, Up
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22271
Homologene: 2524
Ccdc88c
Name: coiled-coil domain containing 88C
Synonyms: Daple, 0610010D24Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 68339
VEGA: 12
Homologene: 18903
Jmjd1c
Name: jumonji domain containing 1C
Synonyms: TRIP8, 5430433L24Rik, D630035I23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 108829
Homologene: 3129
Pip5k1b
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 beta
Synonyms: Pipk5b
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18719
HGNC: HGNC:8995
Homologene: 100644
Mtor
Name: mechanistic target of rapamycin kinase
Synonyms: FKBP-rapamycin-associated protein FRAP, 2610315D21Rik, RAPT1, RAFT1, flat, Frap1, mechanistic target of rapamycin (serine/threonine kinase)
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56717
HGNC: HGNC:3942
Homologene: 3637
Pard3
Name: par-3 family cell polarity regulator
Synonyms: ASIP, D8Ertd580e, PAR-3, Par3, Pard3a
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 93742
Homologene: 10489
Sacm1l
Name: SAC1 suppressor of actin mutations 1-like (yeast)
Synonyms: Sac1p, SAC1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 83493
VEGA: 9
Homologene: 6320
Cdkal1
Name: CDK5 regulatory subunit associated protein 1-like 1
Synonyms: 6620401C13Rik, 1190005B03Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 68916
Homologene: 9830
Ddx55
Name: DEAD box helicase 55
Synonyms: 2810021H22Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 55
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 67848
Homologene: 12196
Plcg1
Name: phospholipase C, gamma 1
Synonyms: Plc-1, Plcg-1, Plc-gamma1, Cded
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18803
HGNC: HGNC:9065
Homologene: 1997
Dgkd
Name: diacylglycerol kinase, delta
Synonyms: DGKdelta, dgkd-2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227333
HGNC: HGNC:2851
Homologene: 100054
Dffa
Name: DNA fragmentation factor, alpha subunit
Synonyms: ICAD-S, ICAD-L, Dff45, DFF35, A330085O09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13347
HGNC: HGNC:2772
Homologene: 3240
Tjp1
Name: tight junction protein 1
Synonyms: ZO-1, ZO1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21872
Homologene: 2445
Mybbp1a
Name: MYB binding protein (P160) 1a
Synonyms: p67MBP, p160MBP
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18432
HGNC: HGNC:7546
Homologene: 40954
Zfp141
Name: zinc finger protein 141
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 434178
Arhgef2
Name: Rho/Rac guanine nucleotide exchange factor 2
Synonyms: Lfc, GEF-H1, LFP40, GEFH1, P40, Lbcl1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 16800
HGNC: HGNC:682
Homologene: 3468
Adam17
Name: a disintegrin and metallopeptidase domain 17
Synonyms: Tace, CD156b
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 11491
HGNC: HGNC:195
Homologene: 2395
Ncoa2
Name: nuclear receptor coactivator 2
Synonyms: TIF2/GRIP-1, Grip1, D1Ertd433e, glucocorticoid receptor-interacting protein 1, TIF2, TIF-2, SRC-2, KAT13C, bHLHe75
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17978
HGNC: HGNC:7669
Homologene: 4768
Nid2
Name: nidogen 2
Synonyms: entactin-2, entactin 2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18074
VEGA: 14
Homologene: 40575
Elf2
Name: E74-like factor 2
Synonyms: NERF-2, 2610036A20Rik, A230104O07Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69257
HGNC: HGNC:3317
Homologene: 5006
Nav1
Name: neuron navigator 1
Synonyms: POMFIL3, 9930003A20Rik, C230080M11Rik, unc53H1, steerin-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 215690
Homologene: 10719
Pomt2
Name: protein-O-mannosyltransferase 2
Synonyms: A830009D15Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217734
VEGA: 12
Homologene: 5297
Tmc2
Name: transmembrane channel-like gene family 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 192140
Homologene: 25877
Nfkb2
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells 2, p49/p100
Synonyms: p52, NF kappaB2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18034
VEGA: 19
HGNC: HGNC:7795
Homologene: 1873
Chst4
Name: carbohydrate sulfotransferase 4
Synonyms: high endothelial cell GlcNAC-6-sulphotransferase, HEC-GlcNAc6ST, GST-3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 26887
HGNC: HGNC:1972
Homologene: 4216
Slc35e2
Name: solute carrier family 35, member E2
Synonyms: A530082C11Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320541
Homologene: 84987
Reln
Name: reelin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Itpkb
Name: inositol 1,4,5-trisphosphate 3-kinase B
Synonyms: 1110033J02Rik, E130307H12Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 320404
HGNC: HGNC:6179
Homologene: 1672
Dnah10
Name: dynein, axonemal, heavy chain 10
Synonyms: Dnahc10
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56087
HGNC: HGNC:2941
Homologene: 25816
Cacna1d
Name: calcium channel, voltage-dependent, L type, alpha 1D subunit
Synonyms: D-LTCC, Cchl1a, Cchl1a2, Cacnl1a2, 8430418G19Rik, Cav1.3alpha1, C79217
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 12289
VEGA: 14
HGNC: HGNC:1391
Homologene: 578
Nalcn
Name: sodium leak channel, non-selective
Synonyms: A530023G15Rik, Vgcnl1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 338370
VEGA: 14
Homologene: 21832
Unc13a
Name: unc-13 homolog A
Synonyms: Munc13-1, 2410078G03Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382018
Homologene: 11279
Gm4787
Name: predicted gene 4787
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 214321
Homologene: 86950
Krba1
Name: KRAB-A domain containing 1
Synonyms: A930040G15Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 77827
Homologene: 15880
Cfap251
Name: cilia and flagella associated protein 251
Synonyms: 4930415N18Rik, 4933428F06Rik, Wdr66
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269701
Homologene: 16964
Cntn4
Name: contactin 4
Synonyms: BIG-2A, Axcam
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 269784
HGNC: HGNC:2174
Homologene: 14257
Ddx60
Name: DExD/H box helicase 60
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234311
Homologene: 23031
Bmi1
Name: Bmi1 polycomb ring finger oncogene
Synonyms: Bmi-1, Bmi1, Pcgf4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12151
Homologene: 3797
Ampd1
Name: adenosine monophosphate deaminase 1
Synonyms: Ampd-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229665
HGNC: HGNC:468
Homologene: 20
Prr5
Name: proline rich 5 (renal)
Synonyms: C030017C09Rik, Protor-1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 109270
Homologene: 17132
Blk
Name: B lymphoid kinase
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 12143
VEGA: 14
HGNC: HGNC:1057
Homologene: 1297
Fscn2
Name: fascin actin-bundling protein 2
Synonyms: C630046B20Rik, ahl8
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 238021
HGNC: HGNC:3960
Homologene: 22722
Vmn2r68
Name: vomeronasal 2, receptor 68
Synonyms: EG620697, Vmn2r68-ps
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 620697
Homologene: 115466
Itga2b
Name: integrin alpha 2b
Synonyms: platelet glycoprotein IIb, GpIIb, alphaIIb, CD41, GP IIb
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16399
HGNC: HGNC:6138
Homologene: 37304
Pnpt1
Name: polyribonucleotide nucleotidyltransferase 1
Synonyms: PNPase, polynucleotide phosphorylase, 1200003F12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71701
Homologene: 12404
Dzank1
Name: double zinc ribbon and ankyrin repeat domains 1
Synonyms: 2810039F03Rik, Ankrd64, 6330439K17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241688
Homologene: 10037
Lrrc8d
Name: leucine rich repeat containing 8D
Synonyms: 4930525N13Rik, Lrrc5, 2810473G09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231549
Homologene: 10004
Aldh3a1
Name: aldehyde dehydrogenase family 3, subfamily A1
Synonyms: Ahd-4, Ahd4, Aldh3, Aldh
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 11670
HGNC: HGNC:405
Homologene: 20175
Pde6b
Name: phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide
Synonyms: rd, r, Pdeb, rd10, rd1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18587
HGNC: HGNC:8786
Homologene: 237
Or5t7
Name: olfactory receptor family 5 subfamily T member 17
Synonyms: GA_x6K02T2Q125-48168771-48167839, MOR179-2, Olfr1086
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258585
Homologene: 73975
Ndufaf7
Name: NADH:ubiquinone oxidoreductase complex assembly factor 7
Synonyms: 2410091C18Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 73694
VEGA: 17
Homologene: 12508
Gtsf1
Name: gametocyte specific factor 1
Synonyms: Cue110, 1700006H03Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 74174
Homologene: 16942
Gm12689
Name: predicted gene 12689
Type: Gene
Species: Mouse
Chromosome: 4
Fcamr
Name: Fc receptor, IgA, IgM, high affinity
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 64435
Homologene: 12929
Cstdc5
Name: cystatin domain containing 5
Synonyms: BC100530
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 100034684
HGNC: HGNC:2481
Homologene: 104045
Nkain2
Name: Na+/K+ transporting ATPase interacting 2
Synonyms: 6330571D19Rik, Tcba1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 432450
Homologene: 65073
Or4c15b
Name: olfactory receptor family 4 subfamily C member 15B
Synonyms: GA_x6K02T2Q125-50727797-50726862, MOR233-19, MOR233-22, Olfr1229
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 257921
Homologene: 79376
Agr2
Name: anterior gradient 2
Synonyms: mAG-2, Gob-4, XAG-2, HAG-2
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 23795
HGNC: HGNC:328
Homologene: 4674
Mucl1
Name: mucin-like 1
Synonyms: Spt-2, Spt2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20771
Homologene: 137219
Gm12966
Name: predicted gene 12966
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 622469
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 13,181,221 bp
  • A to G, chromosome 1 at 87,934,110 bp
  • T to A, chromosome 1 at 130,814,056 bp
  • C to G, chromosome 1 at 135,470,561 bp
  • G to A, chromosome 1 at 180,413,909 bp
  • T to C, chromosome 2 at 18,681,903 bp
  • T to C, chromosome 2 at 86,676,881 bp
  • A to T, chromosome 2 at 89,282,473 bp
  • T to A, chromosome 2 at 130,241,644 bp
  • T to A, chromosome 2 at 144,481,685 bp
  • T to C, chromosome 2 at 160,753,355 bp
  • G to T, chromosome 3 at 51,308,134 bp
  • C to A, chromosome 3 at 88,642,997 bp
  • C to T, chromosome 3 at 103,079,172 bp
  • T to C, chromosome 4 at 99,296,165 bp
  • G to T, chromosome 4 at 129,899,501 bp
  • T to A, chromosome 4 at 148,546,444 bp
  • T to A, chromosome 4 at 149,106,315 bp
  • C to T, chromosome 4 at 155,610,026 bp
  • A to T, chromosome 5 at 21,971,885 bp
  • A to C, chromosome 5 at 76,350,132 bp
  • C to G, chromosome 5 at 105,812,784 bp
  • G to A, chromosome 5 at 105,812,785 bp
  • G to T, chromosome 5 at 108,423,451 bp
  • G to T, chromosome 5 at 123,287,766 bp
  • T to C, chromosome 5 at 124,556,812 bp
  • C to A, chromosome 5 at 124,828,482 bp
  • T to G, chromosome 6 at 48,413,495 bp
  • T to C, chromosome 6 at 106,672,883 bp
  • A to T, chromosome 7 at 42,477,224 bp
  • A to T, chromosome 7 at 65,354,861 bp
  • G to A, chromosome 7 at 85,237,559 bp
  • A to T, chromosome 8 at 61,958,057 bp
  • A to G, chromosome 8 at 71,643,151 bp
  • G to T, chromosome 8 at 110,029,974 bp
  • A to G, chromosome 8 at 127,426,605 bp
  • A to G, chromosome 9 at 57,955,763 bp
  • G to A, chromosome 9 at 75,153,766 bp
  • A to T, chromosome 9 at 123,586,354 bp
  • T to A, chromosome 10 at 31,951,193 bp
  • T to A, chromosome 10 at 67,218,149 bp
  • C to T, chromosome 11 at 9,131,771 bp
  • T to C, chromosome 11 at 29,153,246 bp
  • T to C, chromosome 11 at 61,218,041 bp
  • T to A, chromosome 11 at 72,450,636 bp
  • T to C, chromosome 11 at 98,355,402 bp
  • C to A, chromosome 11 at 102,465,133 bp
  • T to C, chromosome 11 at 120,368,032 bp
  • T to C, chromosome 12 at 21,340,519 bp
  • G to A, chromosome 12 at 35,996,091 bp
  • A to G, chromosome 12 at 81,378,328 bp
  • A to T, chromosome 12 at 87,129,023 bp
  • A to G, chromosome 12 at 100,913,439 bp
  • C to T, chromosome 13 at 29,777,287 bp
  • A to T, chromosome 14 at 19,802,467 bp
  • G to A, chromosome 14 at 30,350,833 bp
  • T to G, chromosome 14 at 63,378,481 bp
  • A to T, chromosome 14 at 123,283,711 bp
  • C to A, chromosome 15 at 84,702,895 bp
  • T to C, chromosome 15 at 103,428,375 bp
  • T to A, chromosome 15 at 103,753,656 bp
  • G to C, chromosome 16 at 36,367,529 bp
  • T to C, chromosome 17 at 78,937,622 bp
  • A to T, chromosome 19 at 24,350,141 bp
  • A to G, chromosome 19 at 27,244,224 bp
  • A to G, chromosome 19 at 46,311,408 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R5514 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
043074-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.