Strain Name:
C57BL/6J-MtgxR1295Btlr/Mmmh
Stock Number:
039361-MU
Citation ID:
RRID:MMRRC_039361-MU
Other Names:
R1295 (G1), C57BL/6J-MtgxR1295Btlr
Major Collection:

Gene Information

Sufu
Name: SUFU negative regulator of hedgehog signaling
Synonyms: Su(Fu), 2810026F04Rik, b2b273Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 24069
Homologene: 9262
Atn1
Name: atrophin 1
Synonyms: Drpla, atrophin-1, Atr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 13498
HGNC: HGNC:3033
Homologene: 1461
Aldh7a1
Name: aldehyde dehydrogenase family 7, member A1
Synonyms: Atq1, D18Wsu181e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 110695
HGNC: HGNC:877
Homologene: 913
Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 1810033J14Rik, 9530077A04Rik, D230038D11Rik, Mll5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 69188
Homologene: 18822
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Cdk18
Name: cyclin-dependent kinase 18
Synonyms: Pctk3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 18557
HGNC: HGNC:8751
Homologene: 1949
Psmb2
Name: proteasome (prosome, macropain) subunit, beta type 2
Synonyms: HC7-I, D4Wsu33e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 26445
HGNC: HGNC:9539
Homologene: 2088
Diaph3
Name: diaphanous related formin 3
Synonyms: p134MDia2, Diap3, Drf3, mDia2, 4930417P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 56419
VEGA: 14
Homologene: 84829
Gstcd
Name: glutathione S-transferase, C-terminal domain containing
Synonyms: 4933434L15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 67553
Homologene: 11693
Ccar1
Name: cell division cycle and apoptosis regulator 1
Synonyms: 2610511G16Rik, Carp1, 9430036H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 67500
VEGA: 10
Homologene: 10086
Mbtd1
Name: mbt domain containing 1
Synonyms: hemp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 103537
Homologene: 41185
Wapl
Name: WAPL cohesin release factor
Synonyms: Wapal, A530089A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 218914
Homologene: 41002
Usp34
Name: ubiquitin specific peptidase 34
Synonyms: Murr2, A530081C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 17847
Homologene: 40978
Cep85
Name: centrosomal protein 85
Synonyms: Ccdc21, 2410030J07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 70012
Homologene: 11254
Ing1
Name: inhibitor of growth family, member 1
Synonyms: mING1h, 2610028J21Rik, p33Ing1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 26356
HGNC: HGNC:6062
Homologene: 40119
Numb
Name: NUMB endocytic adaptor protein
Synonyms: m-numb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 18222
HGNC: HGNC:8060
Homologene: 2775
Arhgef17
Name: Rho guanine nucleotide exchange factor (GEF) 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 207212
Homologene: 129601
Enpp6
Name: ectonucleotide pyrophosphatase/phosphodiesterase 6
Synonyms: 4833421B01Rik, Npp6, D8Ertd514e, B830047L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 320981
Homologene: 62657
Dync2h1
Name: dynein cytoplasmic 2 heavy chain 1
Synonyms: 4432416O06Rik, DHC2, b2b414Clo, Dnchc2, m407Asp, m152Asp, D030010H02Rik, D330044F14Rik, DHC1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 110350
HGNC: HGNC:2962
Homologene: 14468
Zfyve26
Name: zinc finger, FYVE domain containing 26
Synonyms: A630028O16Rik, LOC380767, 9330197E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 211978
VEGA: 12
Homologene: 9102
Stxbp1
Name: syntaxin binding protein 1
Synonyms: Munc-18a, nsec1, Sxtbp1, Munc18-1, Unc18h, N-sec1, Rb-sec1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20910
Homologene: 2382
Amfr
Name: autocrine motility factor receptor
Synonyms: gp78
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 23802
HGNC: HGNC:463
Homologene: 888
Mslnl
Name: mesothelin-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 328783
VEGA: 17
Homologene: 18633
Akap6
Name: A kinase (PRKA) anchor protein 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238161
VEGA: 12
HGNC: HGNC:376
Homologene: 3157
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Itga4
Name: integrin alpha 4
Synonyms: VLA-4 receptor, alpha 4 subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 16401
HGNC: HGNC:6140
Homologene: 37364
Muc6
Name: mucin 6, gastric
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 353328
HGNC: HGNC:7517
Homologene: 18768
Col5a3
Name: collagen, type V, alpha 3
Synonyms: Pro-alpha3(V)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 53867
Homologene: 9253
Vmn2r6
Name: vomeronasal 2, receptor 6
Synonyms: EG620718, EG667069
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 667069
Homologene: 129754
Nav3
Name: neuron navigator 3
Synonyms: 9630020C08Rik, Pomfil1p, 4732483H20Rik, unc53H3, steerin 3, POMFIL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 260315
Homologene: 56688
Arhgap29
Name: Rho GTPase activating protein 29
Synonyms: Parg1, C76601, B130017I01Rik, 6720461J18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 214137
Homologene: 3539
Rmnd5a
Name: required for meiotic nuclear division 5 homolog A
Synonyms: 1110007A06Rik, Gid2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 68477
Homologene: 5668
Ros1
Name: Ros1 proto-oncogene
Synonyms: c-ros, Ros-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 19886
Homologene: 2207
Grik3
Name: glutamate receptor, ionotropic, kainate 3
Synonyms: Glur7, Glur-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 14807
HGNC: HGNC:4581
Homologene: 73901
Vmn2r84
Name: vomeronasal 2, receptor 84
Synonyms: EG625068
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 625068
Homologene: 129606
Gpr22
Name: G protein-coupled receptor 22
Synonyms: 2900068K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 73010
HGNC: HGNC:4477
Homologene: 18420
Ift140
Name: intraflagellar transport 140
Synonyms: Tce5, Wdtc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 106633
Homologene: 40979
Serpina3b
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3B
Synonyms: A030003A19Rik, antitrypsin, alpha-1 antiproteinase, 6A1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 271047
HGNC: HGNC:16
Homologene: 120222
Fam186a
Name: family with sequence similarity 186, member A
Synonyms: 1700030F18Rik, LOC380973
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 72277
Adgrb1
Name: adhesion G protein-coupled receptor B1
Synonyms: Bai1, B830018M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 107831
HGNC: HGNC:943
Homologene: 1287
Rpusd2
Name: RNA pseudouridylate synthase domain containing 2
Synonyms: 4921503C21Rik, BB231107
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 271842
Homologene: 5588
Adprhl1
Name: ADP-ribosylhydrolase like 1
Synonyms: Arh2, D330008N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234072
Homologene: 16311
Rnf19a
Name: ring finger protein 19A
Synonyms: Dorfin, XY body protein, XYbp, Rnf19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 30945
VEGA: 15
Homologene: 8501
Zfp598
Name: zinc finger protein 598
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 213753
VEGA: 17
Homologene: 5672
Ap1m1
Name: adaptor-related protein complex AP-1, mu subunit 1
Synonyms: [m]1A, Cltnm, Adtm1A, AP47, mu1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 11767
Homologene: 4017
Ackr2
Name: atypical chemokine receptor 2
Synonyms: Ccbp2, chemokine decoy receptor D6, D6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 59289
VEGA: 9
HGNC: HGNC:1565
Homologene: 992
Ikbke
Name: inhibitor of kappaB kinase epsilon
Synonyms: IKKepsilon, IKK-i
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 56489
Homologene: 23168
Zp3r
Name: zona pellucida 3 receptor
Synonyms: SP56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 22789
Homologene: 7609
Sele
Name: selectin, endothelial cell
Synonyms: E-selectin, CD62E, Elam
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 20339
Homologene: 389
Ehd3
Name: EH-domain containing 3
Synonyms: Ehd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 57440
HGNC: HGNC:3244
Homologene: 81837
Decr1
Name: 2,4-dienoyl CoA reductase 1, mitochondrial
Synonyms: Nadph, Decr, 1200012F07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 67460
HGNC: HGNC:2753
Homologene: 68178
Vmn1r9
Name: vomeronasal 1 receptor 9
Synonyms: V1rc30
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 171203
Homologene: 76459
Gm6990
Name: predicted pseudogene 6990
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 629643
Ndufaf3
Name: NADH:ubiquinone oxidoreductase complex assembly factor 3
Synonyms: 4733401H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 66706
Homologene: 32460
Atp13a2
Name: ATPase type 13A2
Synonyms: 1110012E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 74772
Homologene: 56940
Haao
Name: 3-hydroxyanthranilate 3,4-dioxygenase
Synonyms: 0610007K21Rik, 3HAO, 0610012J07Rik, 3-HAOxase, 3-HAO
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 107766
VEGA: 17
HGNC: HGNC:4796
Homologene: 8148
AC156643.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
Prodh2
Name: proline dehydrogenase (oxidase) 2
Synonyms: POX1, MmPOX1, 2510038B11Rik, 2510028N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 56189
Homologene: 36381
Gpr61
Name: G protein-coupled receptor 61
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229714
Homologene: 12910
Sall2
Name: spalt like transcription factor 2
Synonyms: Msal-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 50524
Homologene: 9269
Hist1h1b
Name: histone cluster 1, H1b
Synonyms: H1B, H1f5, H1.5, H1s-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 56702
HGNC: HGNC:4719
Homologene: 135981
Tmem56
Name: transmembrane protein 56
Synonyms: C730036B01Rik, 4930577M16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 99887
Homologene: 45107
Tbx2
Name: T-box 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 21385
Homologene: 38123
Thumpd2
Name: THUMP domain containing 2
Synonyms: 2810025A12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 72167
VEGA: 17
Homologene: 11898
Kcnk12
Name: potassium channel, subfamily K, member 12
Synonyms: mntk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 210741
VEGA: 17
HGNC: HGNC:6274
Homologene: 11107
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 130,591,444 bp
  • A to G, chromosome 1 at 131,270,226 bp
  • T to C, chromosome 1 at 132,119,960 bp
  • T to A, chromosome 1 at 164,050,810 bp
  • A to T, chromosome 2 at 32,794,636 bp
  • C to A, chromosome 2 at 76,743,245 bp
  • A to G, chromosome 2 at 79,322,689 bp
  • T to C, chromosome 2 at 119,036,927 bp
  • A to G, chromosome 3 at 64,538,273 bp
  • A to G, chromosome 3 at 108,150,481 bp
  • A to G, chromosome 3 at 121,207,291 bp
  • A to G, chromosome 3 at 121,992,395 bp
  • T to C, chromosome 3 at 133,005,628 bp
  • T to C, chromosome 4 at 15,919,207 bp
  • G to A, chromosome 4 at 125,704,564 bp
  • A to G, chromosome 4 at 126,687,032 bp
  • A to G, chromosome 4 at 134,167,400 bp
  • T to C, chromosome 4 at 140,993,802 bp
  • A to C, chromosome 5 at 23,502,404 bp
  • T to C, chromosome 6 at 57,071,537 bp
  • G to A, chromosome 6 at 71,398,455 bp
  • G to T, chromosome 6 at 124,747,787 bp
  • T to C, chromosome 7 at 30,494,089 bp
  • C to A, chromosome 7 at 100,881,269 bp
  • T to C, chromosome 7 at 141,651,879 bp
  • A to C, chromosome 8 at 11,561,501 bp
  • T to A, chromosome 8 at 11,561,502 bp
  • G to A, chromosome 8 at 13,248,624 bp
  • T to G, chromosome 8 at 47,065,500 bp
  • T to A, chromosome 8 at 72,251,875 bp
  • T to C, chromosome 8 at 93,974,804 bp
  • A to G, chromosome 9 at 7,075,752 bp
  • A to G, chromosome 9 at 20,808,418 bp
  • A to T, chromosome 9 at 53,456,530 bp
  • T to C, chromosome 9 at 108,566,693 bp
  • A to G, chromosome 9 at 121,908,717 bp
  • T to A, chromosome 10 at 52,087,932 bp
  • C to A, chromosome 10 at 62,783,882 bp
  • T to A, chromosome 10 at 109,692,102 bp
  • C to T, chromosome 10 at 130,389,139 bp
  • A to G, chromosome 11 at 23,384,477 bp
  • A to G, chromosome 11 at 85,834,766 bp
  • A to G, chromosome 11 at 93,910,359 bp
  • A to C, chromosome 12 at 31,709,514 bp
  • A to C, chromosome 12 at 52,887,029 bp
  • A to G, chromosome 12 at 79,274,920 bp
  • G to T, chromosome 12 at 82,495,784 bp
  • C to A, chromosome 12 at 83,796,161 bp
  • T to C, chromosome 12 at 104,130,879 bp
  • T to C, chromosome 13 at 21,779,999 bp
  • C to T, chromosome 14 at 34,724,769 bp
  • T to C, chromosome 14 at 52,313,725 bp
  • A to T, chromosome 14 at 87,007,399 bp
  • G to T, chromosome 15 at 36,244,101 bp
  • T to A, chromosome 15 at 74,550,039 bp
  • T to C, chromosome 15 at 99,939,789 bp
  • A to G, chromosome 17 at 24,679,649 bp
  • G to T, chromosome 17 at 25,088,933 bp
  • T to C, chromosome 17 at 25,743,240 bp
  • C to A, chromosome 17 at 73,828,186 bp
  • A to G, chromosome 17 at 81,055,888 bp
  • T to A, chromosome 17 at 83,838,838 bp
  • C to T, chromosome 17 at 87,746,373 bp
  • T to C, chromosome 18 at 56,546,950 bp
  • G to A, chromosome 19 at 46,454,720 bp
  • T to A, chromosome 19 at 56,755,334 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1295 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
039361-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.