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Strain Name:
C57BL/6J-MtgxR1295Btlr/Mmmh
Stock Number:
039361-MU
Citation ID:
RRID:MMRRC_039361-MU
Other Names:
R1295 (G1), C57BL/6J-MtgxR1295Btlr
Major Collection:

Strain Information

Sufu
Name: SUFU negative regulator of hedgehog signaling
Synonyms: Su(Fu), 2810026F04Rik, b2b273Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 24069
Homologene: 9262
Atn1
Name: atrophin 1
Synonyms: atrophin-1, Atr1, Drpla
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13498
HGNC: HGNC:3033
Homologene: 1461
Aldh7a1
Name: aldehyde dehydrogenase family 7, member A1
Synonyms: Atq1, D18Wsu181e
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 110695
HGNC: HGNC:877
Homologene: 913
Kmt2e
Name: lysine (K)-specific methyltransferase 2E
Synonyms: 1810033J14Rik, D230038D11Rik, 9530077A04Rik, Mll5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 69188
Homologene: 18822
Atm
Name: ataxia telangiectasia mutated
Synonyms: C030026E19Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11920
HGNC: HGNC:795
Homologene: 30952
Cdk18
Name: cyclin dependent kinase 18
Synonyms: Pctk3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18557
HGNC: HGNC:8751
Homologene: 1949
Psmb2
Name: proteasome (prosome, macropain) subunit, beta type 2
Synonyms: HC7-I, D4Wsu33e
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 26445
HGNC: HGNC:9539
Homologene: 2088
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 130,591,444 bp
  • A to G, chromosome 1 at 131,270,226 bp
  • T to C, chromosome 1 at 132,119,960 bp
  • T to A, chromosome 1 at 164,050,810 bp
  • A to T, chromosome 2 at 32,794,636 bp
  • C to A, chromosome 2 at 76,743,245 bp
  • A to G, chromosome 2 at 79,322,689 bp
  • T to C, chromosome 2 at 119,036,927 bp
  • A to G, chromosome 3 at 64,538,273 bp
  • A to G, chromosome 3 at 108,150,481 bp
  • A to G, chromosome 3 at 121,207,291 bp
  • A to G, chromosome 3 at 121,992,395 bp
  • T to C, chromosome 3 at 133,005,628 bp
  • T to C, chromosome 4 at 15,919,207 bp
  • G to A, chromosome 4 at 125,704,564 bp
  • A to G, chromosome 4 at 126,687,032 bp
  • A to G, chromosome 4 at 134,167,400 bp
  • T to C, chromosome 4 at 140,993,802 bp
  • A to C, chromosome 5 at 23,502,404 bp
  • T to C, chromosome 6 at 57,071,537 bp
  • G to A, chromosome 6 at 71,398,455 bp
  • G to T, chromosome 6 at 124,747,787 bp
  • T to C, chromosome 7 at 30,494,089 bp
  • C to A, chromosome 7 at 100,881,269 bp
  • T to C, chromosome 7 at 141,651,879 bp
  • A to C, chromosome 8 at 11,561,501 bp
  • T to A, chromosome 8 at 11,561,502 bp
  • G to A, chromosome 8 at 13,248,624 bp
  • T to G, chromosome 8 at 47,065,500 bp
  • T to A, chromosome 8 at 72,251,875 bp
  • T to C, chromosome 8 at 93,974,804 bp
  • A to G, chromosome 9 at 7,075,752 bp
  • A to G, chromosome 9 at 20,808,418 bp
  • A to T, chromosome 9 at 53,456,530 bp
  • T to C, chromosome 9 at 108,566,693 bp
  • A to G, chromosome 9 at 121,908,717 bp
  • T to A, chromosome 10 at 52,087,932 bp
  • C to A, chromosome 10 at 62,783,882 bp
  • T to A, chromosome 10 at 109,692,102 bp
  • C to T, chromosome 10 at 130,389,139 bp
  • A to G, chromosome 11 at 23,384,477 bp
  • A to G, chromosome 11 at 85,834,766 bp
  • A to G, chromosome 11 at 93,910,359 bp
  • A to C, chromosome 12 at 31,709,514 bp
  • A to C, chromosome 12 at 52,887,029 bp
  • A to G, chromosome 12 at 79,274,920 bp
  • G to T, chromosome 12 at 82,495,784 bp
  • C to A, chromosome 12 at 83,796,161 bp
  • T to C, chromosome 12 at 104,130,879 bp
  • T to C, chromosome 13 at 21,779,999 bp
  • C to T, chromosome 14 at 34,724,769 bp
  • T to C, chromosome 14 at 52,313,725 bp
  • A to T, chromosome 14 at 87,007,399 bp
  • G to T, chromosome 15 at 36,244,101 bp
  • T to A, chromosome 15 at 74,550,039 bp
  • T to C, chromosome 15 at 99,939,789 bp
  • A to G, chromosome 17 at 24,679,649 bp
  • G to T, chromosome 17 at 25,088,933 bp
  • T to C, chromosome 17 at 25,743,240 bp
  • C to A, chromosome 17 at 73,828,186 bp
  • A to G, chromosome 17 at 81,055,888 bp
  • T to A, chromosome 17 at 83,838,838 bp
  • C to T, chromosome 17 at 87,746,373 bp
  • T to C, chromosome 18 at 56,546,950 bp
  • G to A, chromosome 19 at 46,454,720 bp
  • T to A, chromosome 19 at 56,755,334 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1295 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
039361-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.