Strain Name:
C57BL/6J-MtgxR4435Btlr/Mmmh
Stock Number:
041149-MU
Citation ID:
RRID:MMRRC_041149-MU
Other Names:
R4435 (G1), C57BL/6J-MtgxR4435Btlr
Major Collection:

Gene Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231051
Homologene: 46480
Cdyl
Name: chromodomain protein, Y chromosome-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 12593
VEGA: 13
HGNC: HGNC:1811
Homologene: 3548
Senp2
Name: SUMO/sentrin specific peptidase 2
Synonyms: 2310007L05Rik, 4930538C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 75826
VEGA: 16
Homologene: 11005
Cyfip1
Name: cytoplasmic FMR1 interacting protein 1
Synonyms: l7Rl1, Sra-1, P140SRA-1, pl-1, Shyc, l(7)1Rl, E030028J09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 20430
Homologene: 22628
Fam169a
Name: family with sequence similarity 169, member A
Synonyms: B230112C05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 320557
VEGA: 13
Homologene: 52656
Zranb2
Name: zinc finger, RAN-binding domain containing 2
Synonyms: Zfp265, Znf265, Zis
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 53861
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: ASC1p200, B630009I04Rik, Helic1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 77987
VEGA: 10
Homologene: 4973
Nedd4l
Name: neural precursor cell expressed, developmentally down-regulated gene 4-like
Synonyms: Nedd4b, Nedd4-2, 1300012C07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 83814
VEGA: 18
HGNC: HGNC:7728
Homologene: 86986
Dennd4c
Name: DENN/MADD domain containing 4C
Synonyms: 1700065A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 329877
Homologene: 23057
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, AnkG, Ankyrin-G, Ankyrin-3, 2900054D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Siah1b
Name: siah E3 ubiquitin protein ligase 1B
Synonyms: Sinh1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 20438
Homologene: 56441
Strip2
Name: striatin interacting protein 2
Synonyms: D330017J20Rik, Fam40b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 320609
Homologene: 66198
Hk1
Name: hexokinase 1
Synonyms: Hk1-s, Hk-1, mHk1-s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 15275
HGNC: HGNC:4922
Homologene: 100530
Uimc1
Name: ubiquitin interaction motif containing 1
Synonyms: D630032M02Rik, 9430016E08Rik, D330018D10Rik, Rxrip110
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 20184
Homologene: 9455
Gpn3
Name: GPN-loop GTPase 3
Synonyms: Atpbd1c, D5Ertd708e, A930018B01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 68080
Homologene: 6487
Bccip
Name: BRCA2 and CDKN1A interacting protein
Synonyms: TOK-1, 1110013J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 66165
HGNC: HGNC:978
Homologene: 41629
Arap1
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
Synonyms: Centd2, 2410002L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 69710
Homologene: 12326
Robo4
Name: roundabout guidance receptor 4
Synonyms: 1200012D01Rik, Magic roundabout
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 74144
Homologene: 10397
Tsc2
Name: TSC complex subunit 2
Synonyms: tuberin, Nafld, tuberous sclerosis 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 22084
VEGA: 17
Homologene: 462
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Psd
Name: pleckstrin and Sec7 domain containing
Synonyms: Psdl, 1110007H17Rik, Efa6, Efa6a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 73728
VEGA: 19
HGNC: HGNC:9507
Homologene: 31115
Ptprq
Name: protein tyrosine phosphatase, receptor type, Q
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237523
HGNC: HGNC:9679
Homologene: 83557
Fam135b
Name: family with sequence similarity 135, member B
Synonyms: A830008O07Rik, 1700010C24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 70363
VEGA: 15
Homologene: 66605
Slc38a4
Name: solute carrier family 38, member 4
Synonyms: Ata3, 1110012E16Rik, 1700012A18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 69354
VEGA: 15
Homologene: 75077
Nwd1
Name: NACHT and WD repeat domain containing 1
Synonyms: A230063L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 319555
Homologene: 72261
Gm5134
Name: predicted gene 5134
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 333669
Homologene: 129891
Sos2
Name: SOS Ras/Rho guanine nucleotide exchange factor 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 20663
Homologene: 38253
Dcaf11
Name: DDB1 and CUL4 associated factor 11
Synonyms: D14Ucla1, GLO14, 0710008A13Rik, Wdr23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 28199
Homologene: 11886
Ciao3
Name: cytosolic iron-sulfur assembly component 3
Synonyms: Narfl, 9030612I22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 67563
Homologene: 6750
Ccndbp1
Name: cyclin D-type binding-protein 1
Synonyms: Maid, DIP1, SSEC-8, GCIP, stage specific embryonic cDNA-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 17151
HGNC: HGNC:1587
Homologene: 7826
Asrgl1
Name: asparaginase like 1
Synonyms: ALP1, 2410004D18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 66514
Homologene: 11825
Adam6b
Name: a disintegrin and metallopeptidase domain 6B
Synonyms: 4930523C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238405
Homologene: 128362
Maf
Name: avian musculoaponeurotic fibrosarcoma oncogene homolog
Synonyms: A230108G15Rik, c-maf, 2810401A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 17132
HGNC: HGNC:6776
Homologene: 74552
Zswim3
Name: zinc finger SWIM-type containing 3
Synonyms: 4921517A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 67538
Homologene: 15436
Olfr290
Name: olfactory receptor 290
Synonyms: MOR254-1, GA_x6K02T2NHDJ-11170115-11169168
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258411
Homologene: 83130
Arhgap25
Name: Rho GTPase activating protein 25
Synonyms: A130039I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232201
Homologene: 45507
Myrip
Name: myosin VIIA and Rab interacting protein
Synonyms: A230081N12Rik, Slac2-c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 245049
Homologene: 9150
Ifih1
Name: interferon induced with helicase C domain 1
Synonyms: MDA-5, Helicard, MDA5, 9130009C22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 71586
Homologene: 32535
Gm20388
Name: predicted gene 20388
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
Olfr446
Name: olfactory receptor 446
Synonyms: GA_x6K02T2P3E9-4632269-4631343, MOR261-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 258292
Homologene: 17179
Asnsd1
Name: asparagine synthetase domain containing 1
Synonyms: 2210409M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 70396
Homologene: 6564
Gm5849
Name: predicted gene 5849
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 53,348,073 bp
  • A to G, chromosome 2 at 62,645,890 bp
  • T to C, chromosome 2 at 76,916,875 bp
  • T to A, chromosome 2 at 121,008,470 bp
  • T to A, chromosome 2 at 164,820,643 bp
  • T to A, chromosome 3 at 90,777,875 bp
  • T to A, chromosome 3 at 157,546,741 bp
  • C to A, chromosome 4 at 86,798,075 bp
  • A to T, chromosome 5 at 25,314,877 bp
  • A to G, chromosome 5 at 122,382,052 bp
  • T to C, chromosome 6 at 29,925,050 bp
  • T to A, chromosome 6 at 42,928,089 bp
  • T to C, chromosome 6 at 87,462,938 bp
  • T to C, chromosome 7 at 55,900,041 bp
  • A to G, chromosome 7 at 84,916,021 bp
  • C to A, chromosome 7 at 101,390,254 bp
  • A to G, chromosome 7 at 133,719,213 bp
  • T to C, chromosome 8 at 72,688,136 bp
  • T to A, chromosome 8 at 115,706,853 bp
  • T to C, chromosome 8 at 122,413,952 bp
  • CGG to CG, chromosome 9 at 37,411,490 bp
  • C to T, chromosome 9 at 120,335,614 bp
  • T to A, chromosome 10 at 50,721,885 bp
  • A to G, chromosome 10 at 62,275,844 bp
  • C to T, chromosome 10 at 69,987,070 bp
  • T to G, chromosome 10 at 75,995,824 bp
  • A to G, chromosome 10 at 107,685,055 bp
  • T to C, chromosome 12 at 69,614,699 bp
  • A to C, chromosome 12 at 113,490,661 bp
  • T to C, chromosome 13 at 35,858,250 bp
  • T to C, chromosome 13 at 55,075,823 bp
  • A to G, chromosome 13 at 97,126,740 bp
  • T to A, chromosome 14 at 55,565,566 bp
  • T to C, chromosome 15 at 71,448,739 bp
  • T to C, chromosome 15 at 97,009,018 bp
  • T to A, chromosome 16 at 22,014,241 bp
  • G to A, chromosome 17 at 24,599,713 bp
  • C to A, chromosome 17 at 25,781,797 bp
  • A to G, chromosome 18 at 65,212,825 bp
  • C to T, chromosome 19 at 9,119,199 bp
  • A to T, chromosome 19 at 46,314,494 bp
  • G to A, chromosome X at 164,071,692 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4435 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041149-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.