Strain Name:
C57BL/6J-MtgxR4435Btlr/Mmmh
Stock Number:
041149-MU
Citation ID:
RRID:MMRRC_041149-MU
Other Names:
R4435 (G1), C57BL/6J-MtgxR4435Btlr
Major Collection:

Strain Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: HALR, E330008K23Rik, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Cdyl
Name: chromodomain protein, Y chromosome-like
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 12593
VEGA: 13
HGNC: HGNC:1811
Homologene: 3548
Senp2
Name: SUMO/sentrin specific peptidase 2
Synonyms: 4930538C18Rik, 2310007L05Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 75826
VEGA: 16
Homologene: 11005
Cyfip1
Name: cytoplasmic FMR1 interacting protein 1
Synonyms: Sra-1, l(7)1Rl, P140SRA-1, E030028J09Rik, Shyc, pl-1, l7Rl1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20430
Homologene: 22628
Fam169a
Name: family with sequence similarity 169, member A
Synonyms: B230112C05Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 320557
VEGA: 13
Homologene: 52656
Zranb2
Name: zinc finger, RAN-binding domain containing 2
Synonyms: Zis, Zfp265, Znf265
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 53861
Ascc3
Name: activating signal cointegrator 1 complex subunit 3
Synonyms: B630009I04Rik, Helic1, ASC1p200
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77987
VEGA: 10
Homologene: 4973
Nedd4l
Name: neural precursor cell expressed, developmentally down-regulated gene 4-like
Synonyms: Nedd4b, 1300012C07Rik, Nedd4-2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 83814
VEGA: 18
HGNC: HGNC:7728
Homologene: 86986
Dennd4c
Name: DENN domain containing 4C
Synonyms: 1700065A05Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 329877
Homologene: 23057
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, AnkG, 2900054D09Rik, Ankyrin-3, Ankyrin-G
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Siah1b
Name: siah E3 ubiquitin protein ligase 1B
Synonyms: Sinh1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 20438
Homologene: 56441
Strip2
Name: striatin interacting protein 2
Synonyms: Fam40b, Myoscape, D330017J20Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320609
Homologene: 66198
Hk1
Name: hexokinase 1
Synonyms: Hk-1, mHk1-s, Hk1-s
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 15275
HGNC: HGNC:4922
Homologene: 100530
Uimc1
Name: ubiquitin interaction motif containing 1
Synonyms: Rxrip110, D630032M02Rik, D330018D10Rik, 9430016E08Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20184
Homologene: 9455
Gpn3
Name: GPN-loop GTPase 3
Synonyms: D5Ertd708e, A930018B01Rik, Atpbd1c
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68080
Homologene: 6487
Bccip
Name: BRCA2 and CDKN1A interacting protein
Synonyms: TOK-1, 1110013J05Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 66165
HGNC: HGNC:978
Homologene: 41629
Arap1
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
Synonyms: 2410002L19Rik, Centd2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 69710
Homologene: 12326
Robo4
Name: roundabout guidance receptor 4
Synonyms: 1200012D01Rik, Magic roundabout
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74144
Homologene: 10397
Tsc2
Name: TSC complex subunit 2
Synonyms: tuberous sclerosis 2, tuberin, Nafld
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22084
VEGA: 17
Homologene: 462
Ttn
Name: titin
Synonyms: shru, L56, mdm, connectin, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, D830007G01Rik, 1100001C23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Psd
Name: pleckstrin and Sec7 domain containing
Synonyms: Psdl, Efa6, Efa6a, 1110007H17Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 73728
VEGA: 19
HGNC: HGNC:9507
Homologene: 31115
Ptprq
Name: protein tyrosine phosphatase receptor type Q
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 237523
HGNC: HGNC:9679
Homologene: 83557
Fam135b
Name: family with sequence similarity 135, member B
Synonyms: 1700010C24Rik, A830008O07Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 70363
VEGA: 15
Homologene: 66605
Slc38a4
Name: solute carrier family 38, member 4
Synonyms: SNAT4, Ata3, 1700012A18Rik, 1110012E16Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 69354
VEGA: 15
Homologene: 75077
Nwd1
Name: NACHT and WD repeat domain containing 1
Synonyms: A230063L24Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 319555
Homologene: 72261
Gm5134
Name: predicted gene 5134
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 333669
Homologene: 129891
Sos2
Name: SOS Ras/Rho guanine nucleotide exchange factor 2
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20663
Homologene: 38253
Dcaf11
Name: DDB1 and CUL4 associated factor 11
Synonyms: D14Ucla1, GLO14, 0710008A13Rik, Wdr23
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 28199
Homologene: 11886
Ciao3
Name: cytosolic iron-sulfur assembly component 3
Synonyms: 9030612I22Rik, Narfl
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 67563
Homologene: 6750
Ccndbp1
Name: cyclin D-type binding-protein 1
Synonyms: stage specific embryonic cDNA-8, Maid, DIP1, GCIP, SSEC-8
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 17151
HGNC: HGNC:1587
Homologene: 7826
Asrgl1
Name: asparaginase like 1
Synonyms: 2410004D18Rik, ALP1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 66514
Homologene: 11825
Adam6b
Name: a disintegrin and metallopeptidase domain 6B
Synonyms: 4930523C11Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238405
Homologene: 128362
Maf
Name: MAF bZIP transcription factor
Synonyms: 2810401A20Rik, A230108G15Rik, c-maf
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 17132
HGNC: HGNC:6776
Homologene: 74552
Zswim3
Name: zinc finger SWIM-type containing 3
Synonyms: 4921517A06Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67538
Homologene: 15436
Or5ae1
Name: olfactory receptor family 5 subfamily AE member 1
Synonyms: Olfr290, MOR254-1, GA_x6K02T2NHDJ-11170115-11169168
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258411
Homologene: 83130
Arhgap25
Name: Rho GTPase activating protein 25
Synonyms: A130039I20Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232201
Homologene: 45507
Myrip
Name: myosin VIIA and Rab interacting protein
Synonyms: A230081N12Rik, Slac2-c
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 245049
Homologene: 9150
Ifih1
Name: interferon induced with helicase C domain 1
Synonyms: MDA-5, Helicard, 9130009C22Rik, MDA5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71586
Homologene: 32535
Galnt2l
Name: polypeptide N-acetylgalactosaminyltransferase 2-like
Synonyms: Gm20388
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Or2a12
Name: olfactory receptor family 2 subfamily A member 12
Synonyms: GA_x6K02T2P3E9-4632269-4631343, MOR261-12, Olfr446
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 258292
Homologene: 17179
Asnsd1
Name: asparagine synthetase domain containing 1
Synonyms: 2210409M21Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 70396
Homologene: 6564
Gm5849
Name: predicted gene 5849
Type: Gene
Species: Mouse
Chromosome: 3
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 53,348,073 bp
  • A to G, chromosome 2 at 62,645,890 bp
  • T to C, chromosome 2 at 76,916,875 bp
  • T to A, chromosome 2 at 121,008,470 bp
  • T to A, chromosome 2 at 164,820,643 bp
  • T to A, chromosome 3 at 90,777,875 bp
  • T to A, chromosome 3 at 157,546,741 bp
  • C to A, chromosome 4 at 86,798,075 bp
  • A to T, chromosome 5 at 25,314,877 bp
  • A to G, chromosome 5 at 122,382,052 bp
  • T to C, chromosome 6 at 29,925,050 bp
  • T to A, chromosome 6 at 42,928,089 bp
  • T to C, chromosome 6 at 87,462,938 bp
  • T to C, chromosome 7 at 55,900,041 bp
  • A to G, chromosome 7 at 84,916,021 bp
  • C to A, chromosome 7 at 101,390,254 bp
  • A to G, chromosome 7 at 133,719,213 bp
  • T to C, chromosome 8 at 72,688,136 bp
  • T to A, chromosome 8 at 115,706,853 bp
  • T to C, chromosome 8 at 122,413,952 bp
  • CGG to CG, chromosome 9 at 37,411,490 bp
  • C to T, chromosome 9 at 120,335,614 bp
  • T to A, chromosome 10 at 50,721,885 bp
  • A to G, chromosome 10 at 62,275,844 bp
  • C to T, chromosome 10 at 69,987,070 bp
  • T to G, chromosome 10 at 75,995,824 bp
  • A to G, chromosome 10 at 107,685,055 bp
  • T to C, chromosome 12 at 69,614,699 bp
  • A to C, chromosome 12 at 113,490,661 bp
  • T to C, chromosome 13 at 35,858,250 bp
  • T to C, chromosome 13 at 55,075,823 bp
  • A to G, chromosome 13 at 97,126,740 bp
  • T to A, chromosome 14 at 55,565,566 bp
  • T to C, chromosome 15 at 71,448,739 bp
  • T to C, chromosome 15 at 97,009,018 bp
  • T to A, chromosome 16 at 22,014,241 bp
  • G to A, chromosome 17 at 24,599,713 bp
  • C to A, chromosome 17 at 25,781,797 bp
  • A to G, chromosome 18 at 65,212,825 bp
  • C to T, chromosome 19 at 9,119,199 bp
  • A to T, chromosome 19 at 46,314,494 bp
  • G to A, chromosome X at 164,071,692 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4435 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041149-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.